Apolipoprotein D (APOD) (NM_001647) Human Tagged ORF Clone
APOD (Myc-DDK-tagged)-Human apolipoprotein D (APOD)
|Product Name||Apolipoprotein D (APOD) (NM_001647) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC200503 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC200503 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||567 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at email@example.com or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq||NM_001647.1, NM_001647.2, NM_001647.3, NP_001638.1|
|RefSeq Size||1148 bp|
|RefSeq ORF||570 bp|
|Protein Families||Secreted Protein|
|Gene Summary||This gene encodes a component of high density lipoprotein that has no marked similarity to other apolipoprotein sequences. It has a high degree of homology to plasma retinol-binding protein and other members of the alpha 2 microglobulin protein superfamily of carrier proteins, also known as lipocalins. This glycoprotein is closely associated with the enzyme lecithin:cholesterol acyltransferase - an enzyme involved in lipoprotein metabolism. [provided by RefSeq, Aug 2008]|
|The use of this cDNA Clones has been cited in the following citations:|
Apolipoprotein D Upregulation in Alzheimer's Disease but Not Frontotemporal Dementia
,Bhatia, S;Kim, WS;Shepherd, CE;Halliday, GM;,
J. Mol. Neurosci. 2018
,PubMed ID 30467822
|cDNA Clone Resources|
|SC127272||APOD (untagged)-Human apolipoprotein D (APOD)||
|RG200503||APOD (GFP-tagged) - Human apolipoprotein D (APOD)||
|RC200503L1||Lenti ORF clone of Human apolipoprotein D (APOD), Myc-DDK-tagged||
|RC200503L2||Lenti ORF clone of Human apolipoprotein D (APOD), mGFP tagged||
|RC200503L3||Lenti ORF clone of Human apolipoprotein D (APOD), Myc-DDK-tagged||
|RC200503L4||Lenti ORF clone of Human apolipoprotein D (APOD), mGFP tagged||