Apolipoprotein D (APOD) (NM_001647) Human Untagged Clone

CAT#: SC127272

APOD (untagged)-Human apolipoprotein D (APOD)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Apolipoprotein D
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127272 sequence for NM_001647 edited (data generated by NextGen Sequencing)
  ATGGTGATGCTGCTGCTGCTGCTTTCCGCACTGGCTGGCCTCTTCGGTGCGGCAGAGGGA
  CAAGCATTTCATCTTGGGAAGTGCCCCAATCCTCCGGTGCAGGAGAATTTTGACGTGAAT
  AAGTATCTCGGAAGATGGTACGAAATTGAGAAGATCCCAACAACCTTTGAGAATGGACGC
  TGCATCCAGGCCAACTACTCACTAATGGAAAACGGAAAGATCAAAGTGTTAAACCAGGAG
  TTGAGAGCTGATGGAACTGTGAATCAAATCGAAGGTGAAGCCACCCCAGTTAACCTCACA
  GAGCCTGCCAAGCTGGAAGTTAAGTTTTCCTGGTTTATGCCATCGGCACCGTACTGGATC
  CTGGCCACCGACTATGAGAACTATGCCCTCGTGTATTCCTGTACCTGCATCATCCAACTT
  TTTCACGTGGATTTTGCTTGGATCTTGGCAAGAAACCCTAATCTCCCTCCAGAAACAGTG
  GACTCTCTAAAAAATATCCTGACTTCTAATAACATTGATGTCAAGAAAATGACGGTCACA
  GACCAGGTGAACTGCCCCAAGCTCTCGTAA
  
  Clone variation with respect to NM_001647.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001647 unedited
  TACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCAAGACAGGCATCTCAAATC
  GGCTGATTCTGCATCTGGAAACTGCCTTCATCTTGAAAGAAAAGCTCCAGGTCCCTTCTC
  CAGCCACCCAGCCCCAAGATGGTGATGCTGCTGCTGCTGCTTTCCGCACTGGCTGGCCTC
  TTCGGTGCGGCAGAGGGACAAGCATTTCATCTTGGGAAGTGCCCCAATCCTCCGGTGCAG
  GAGAATTTTGACGTGAATAAGTATCTCGGAAGATGGTACGAAATTGAGAAGATCCCAACA
  ACCTTTGAGAATGGACGCTGCATCCAGGCCAACTACTCACTAATGGAAAACGGAAAGATC
  AAAGTGTTAAACCAGGAGTTGAGAGCTGATGGAACTGTGAATCAAATCGAAGGTGAAGCC
  ACCCCAGTTAACCTCACAGAGCCTGCCAAGCTGGAAGTTAAGTTTTCCTGGTTTATGCCA
  TCGGCACCGTACTGGATCCTGGCCACCGACTATGAGAACTATGCCCTCGTGTATTCCTGT
  ACCTGCATCATCCAACTTTTTCACGTGGATTTTGCTTGGATCTTGGCAAGAAACCCTAAT
  CTCCCTCCAGAAACAGTGGACTCTCTAAAAAATATCCTGACTTCTAATAACATTGATGTC
  AAGAAAATGACGGTCACAGACCAGGTGAACTGCCCCAAGCTCTCGTAACCAGNTTCTACA
  GGGAGGCTGCACCCACTCCATGTTACTTCTGTTTGGCTTTCCCCTACCCCACCCCCCCCC
  ATANAGACAAACCAATCAACCCGACAAAGGAAGTTGACCTAAACATGTACCCCATGCCTA
  CCCTGNTACCTTGCTAGCTGCAAATAAAACTGNTGCTGACCTGCTGTGCTCGCAAAAAAA
  AAAAAAAAAAAAAAAAAAAACTCGCTCTAGATGCGGCCGCGTCATAGCTGTTCCTGAAAG
  AACC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001647
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001647.2, NP_001638.1
• RefSeq Size: 1061 bp
• RefSeq ORF: 570 bp
• Locus ID: 347
• UniProt ID: P05090
• Cytogenetics: 3q29
• Domains: lipocalin
• Protein Families: Secreted Protein
• Gene Summary: This gene encodes a component of high density lipoprotein that has no marked similarity to other apolipoprotein sequences. It has a high degree of homology to plasma retinol-binding protein and other members of the alpha 2 microglobulin protein superfamily of carrier proteins, also known as lipocalins. This glycoprotein is closely associated with the enzyme lecithin:cholesterol acyltransferase - an enzyme involved in lipoprotein metabolism. [provided by RefSeq, Aug 2008]