BNIP2 (NM_004330) Human Tagged ORF Clone
CAT#: RC200377
BNIP2 (Myc-DDK-tagged)-Human BCL2/adenovirus E1B 19kDa interacting protein 2 (BNIP2)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_004330" in other vectors (5)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | BNIP2 |
Synonyms | BNIP-2; NIP2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC200377 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGGTGTGGAACTTAAAGAAGAATGGCAAGATGAAGATTTTCCGATACCTTTACCAGAAGATGATA GTATTGAAGCAGATATACTAGCTATAACTGGACCAGAGGACCAGCCTGGCTCACTAGAAGTTAATGGAAA TAAAGTGAGAAAGAAACTAATGGCTCCAGACATTAGCCTGACACTGGATCCTAGTGATGGCTCTGTATTG TCAGATGATTTGGATGAAAGTGGGGAGATTGACTTAGATGGCTTAGACACACCGTCAGAGAATAGTAATG AGTTTGAGTGGGAAGATGATCTTCCAAAACCCAAGACTACTGAAGTAATTAGGAAAGGCTCAATTACTGA ATACACAGCAGCAGAGGAAAAAGAAGATGGACGACGCTGGCGTATGTTCAGGATTGGAGAACAGGACCAC AGGGTTGATATGAAGGCAATTGAACCCTATAAAAAAGTTATCAGCCATGGGGGATATTATGGGGATGGAT TAAATGCCATTGTTGTGTTTGCTGTCTGTTTCATGCCTGAAAGTAGTCAGCCTAACTATAGATACCTGAT GGACAATCTTTTTAAATATGTTATTGGCACTTTGGAGCTATTAGTAGCAGAAAACTACATGATAGTTTAT TTAAATGGTGCAACAACTCGAAGAAAAATGCCCAGTCTGGGATGGCTCAGGAAATGTTATCAGCAAATTG ATAGAAGGTTACGGAAAAATCTAAAATCCCTAATCATTGTACATCCTTCTTGGTTTATCAGAACACTTCT GGCTGTTACAAGACCATTTATTAGCTCGAAATTCAGCCAAAAAATTAGATACGTGTTTAATTTGGCAGAA CTAGCAGAACTTGTCCCCATGGAATACGTTGGCATACCAGAATGCATAAAACAAGTTGATCAAGAACTTA ATGGAAAACAAGATGAACCGAAAAATGAACAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200377 protein sequence
Red=Cloning site Green=Tags(s) MEGVELKEEWQDEDFPIPLPEDDSIEADILAITGPEDQPGSLEVNGNKVRKKLMAPDISLTLDPSDGSVL SDDLDESGEIDLDGLDTPSENSNEFEWEDDLPKPKTTEVIRKGSITEYTAAEEKEDGRRWRMFRIGEQDH RVDMKAIEPYKKVISHGGYYGDGLNAIVVFAVCFMPESSQPNYRYLMDNLFKYVIGTLELLVAENYMIVY LNGATTRRKMPSLGWLRKCYQQIDRRLRKNLKSLIIVHPSWFIRTLLAVTRPFISSKFSQKIRYVFNLAE LAELVPMEYVGIPECIKQVDQELNGKQDEPKNEQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_004330 |
ORF Size | 942 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_004330.4 |
RefSeq Size | 2533 bp |
RefSeq ORF | 945 bp |
Locus ID | 663 |
UniProt ID | Q12982 |
Cytogenetics | 15q22.2 |
Domains | SEC14 |
MW | 36 kDa |
Gene Summary | This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein, which protects cells from virally-induced cell death. The encoded protein also interacts with E1B 19 kDa-like sequences of BCL2, another apoptotic protector. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC200377L3 | Lenti ORF clone of Human BCL2/adenovirus E1B 19kDa interacting protein 2 (BNIP2), Myc-DDK-tagged |
USD 600.00 |
|
RC200377L4 | Lenti ORF clone of Human BCL2/adenovirus E1B 19kDa interacting protein 2 (BNIP2), mGFP tagged |
USD 600.00 |
|
RG200377 | BNIP2 (tGFP-tagged) - Human BCL2/adenovirus E1B 19kDa interacting protein 2 (BNIP2) |
USD 500.00 |
|
SC125519 | BNIP2 (untagged)-Human BCL2/adenovirus E1B 19kDa interacting protein 2 (BNIP2) |
USD 300.00 |
|
SC317709 | BNIP2 (untagged)-Human BCL2/adenovirus E1B 19kDa interacting protein 2 (BNIP2) |
USD 503.00 |
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