Kcnab1 (NM_001289450) Mouse Tagged ORF Clone
CAT#: MR229076
- TrueORF®
Kcnab1 (myc-DDK-tagged) - Mouse potassium voltage-gated channel, shaker-related subfamily, beta member 1 (Kcnab1), transcript variant 2
"NM_001289450" in other vectors (1)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Kcnab1 |
Synonyms | Akr8a8; Kvbeta1.1; mKv(beta)1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR229076 representing NM_001289450
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAATTGCCTACGAAAGTGGAGTTAATCTCTTCGACACAGCTGAGGTCTATGCTGCTGGGAAGGCTG AGGTGATTCTGGGAAGCATCATCAAGAAGAAAGGCTGGAGGAGGTCCAGCTTGGTCATCACAACCAAACT CTACTGGGGTGGAAAAGCTGAGACAGAAAGGGGACTGTCAAGAAAGCACATCATTGAAGGACTGAAAGGC TCCCTCCAGAGGCTGCAACTGGAATACGTGGATGTGGTCTTTGCAAATCGCCCAGACAGCAACACTCCCA TGGAAGAAATCGTTCGAGCCATGACGCACGTGATCAACCAAGGCATGGCCATGTACTGGGGCACCTCGAG GTGGAGCGCGATGGAGATCATGGAAGCCTACTCTGTCGCACGGCAGTTCAACATGATCCCGCCTGTCTGT GAGCAAGCTGAGTACCATCTTTTCCAGAGAGAGAAGGTGGAGGTCCAGCTGCCGGAGCTCTACCATAAAA TAGGAGTTGGTGCAATGACATGGTCTCCACTTGCTTGTGGAATTATTTCAGGAAAATATGGAAATGGGGT GCCAGAAAGTTCTAGAGCTTCACTGAAGTGCTACCAGTGGTTGAAGGAAAGAATCGTAAGTGAAGAAGGG AGAAAACAGCAAAACAAGCTGAAAGACCTCTCTCCAATCGCTGAGCGCCTGGGGTGCACGCTACCTCAGC TGGCTGTGGCGTGGTGCCTGAGAAATGAGGGTGTGAGTTCTGTGCTCCTGGGATCATCCACTCCGGAACA ACTCATTGAAAACCTTGGTGCCATTCAGGTCCTCCCTAAGATGACATCTCACGTGGTGAACGAGATTGAT AACATACTGCGCAACAAGCCCTACAGCAAAAAGGACTATAGATCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR229076 representing NM_001289450
Red=Cloning site Green=Tags(s) MTIAYESGVNLFDTAEVYAAGKAEVILGSIIKKKGWRRSSLVITTKLYWGGKAETERGLSRKHIIEGLKG SLQRLQLEYVDVVFANRPDSNTPMEEIVRAMTHVINQGMAMYWGTSRWSAMEIMEAYSVARQFNMIPPVC EQAEYHLFQREKVEVQLPELYHKIGVGAMTWSPLACGIISGKYGNGVPESSRASLKCYQWLKERIVSEEG RKQQNKLKDLSPIAERLGCTLPQLAVAWCLRNEGVSSVLLGSSTPEQLIENLGAIQVLPKMTSHVVNEID NILRNKPYSKKDYRS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001289450 |
ORF Size | 885 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001289450.1, NP_001276379.1 |
RefSeq Size | 2851 bp |
RefSeq ORF | 888 bp |
Locus ID | 16497 |
Cytogenetics | 3 30.15 cM |
MW | 33.6 kDa |
Gene Summary | Cytoplasmic potassium channel subunit that modulates the characteristics of the channel-forming alpha-subunits (PubMed:10454353). Modulates action potentials via its effect on the pore-forming alpha subunits (PubMed:10454353). Promotes expression of the pore-forming alpha subunits at the cell membrane, and thereby increases channel activity (PubMed:8824288). Mediates closure of delayed rectifier potassium channels by physically obstructing the pore via its N-terminal domain and increases the speed of channel closure for other family members (By similarity). Promotes the closure of KCNA1, KCNA2 and KCNA5 channels (By similarity). Accelerates KCNA4 channel closure (By similarity). Accelerates the closure of heteromeric channels formed by KCNA1 and KCNA4 (By similarity). Accelerates the closure of heteromeric channels formed by KCNA2, KCNA5 and KCNA6 (By similarity). Enhances KCNB1 and KCNB2 channel activity (PubMed:8824288). Binds NADPH; this is required for efficient down-regulation of potassium channel activity (By similarity). Has NADPH-dependent aldoketoreductase activity (By similarity). Oxidation of the bound NADPH strongly decreases N-type inactivation of potassium channel activity (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC226865 | Kcnab1 (untagged) - Mouse potassium voltage-gated channel, shaker-related subfamily, beta member 1 (Kcnab1), transcript variant 2 |
USD 330.00 |
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