Tgfb1 (NM_011577) Mouse Tagged ORF Clone

CAT#: MR227339

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  • TrueORF®

Tgfb1 (Myc-DDK-tagged) - Mouse transforming growth factor, beta 1 (Tgfb1)



  "NM_011577" in other vectors (5)

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Reconstitution Protocol

USD 686.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Tgfb1
Synonyms TGF-beta; TGF-beta1; Tgfb; Tgfb-1; TGFbeta; TGFbeta1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR227339 representing NM_011577
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGCCCTCGGGGCTGCGGCTACTGCCGCTTCTGCTCCCACTCCCGTGGCTTCTAGTGCTGACGCCCG
GGAGGCCAGCCGCGGGACTCTCCACCTGCAAGACCATCGACATGGAGCTGGTGAAACGGAAGCGCATCGA
AGCCATCCGTGGCCAGATCCTGTCCAAACTAAGGCTCGCCAGTCCCCCAAGCCAGGGGGAGGTACCGCCC
GGCCCGCTGCCCGAGGCGGTGCTCGCTTTGTACAACAGCACCCGCGACCGGGTGGCAGGCGAGAGCGCCG
ACCCAGAGCCGGAGCCCGAAGCGGACTACTATGCTAAAGAGGTCACCCGCGTGCTAATGGTGGACCGCAA
CAACGCCATCTATGAGAAAACCAAAGACATCTCACACAGTATATATATGTTCTTCAATACGTCAGACATT
CGGGAAGCAGTGCCCGAACCCCCATTGCTGTCCCGTGCAGAGCTGCGCTTGCAGAGATTAAAATCAAGTG
TGGAGCAACATGTGGAACTCTACCAGAAATATAGCAACAATTCCTGGCGTTACCTTGGTAACCGGCTGCT
GACCCCCACTGATACGCCTGAGTGGCTGTCTTTTGACGTCACTGGAGTTGTACGGCAGTGGCTGAACCAA
GGAGACGGAATACAGGGCTTTCGATTCAGCGCTCACTGCTCTTGTGACAGCAAAGATAACAAACTCCACG
TGGAAATCAACGGGATCAGCCCCAAACGTCGGGGCGACCTGGGCACCATCCATGACATGAACCGGCCCTT
CCTGCTCCTCATGGCCACCCCCCTGGAAAGGGCCCAGCACCTGCACAGCTCACGGCACCGGAGAGCCCTG
GATACCAACTATTGCTTCAGCTCCACAGAGAAGAACTGCTGTGTGCGGCAGCTGTACATTGACTTTAGGA
AGGACCTGGGTTGGAAGTGGATCCACGAGCCCAAGGGCTACCATGCCAACTTCTGTCTGGGACCCTGCCC
CTATATTTGGAGCCTGGACACACAGTACAGCAAGGTCCTTGCCCTCTACAACCAACACAACCCGGGCGCT
TCGGCGTCACCGTGCTGCGTGCCGCAGGCTTTGGAGCCACTGCCCATCGTCTACTACGTGGGTCGCAAGC
CCAAGGTGGAGCAGTTGTCCAACATGATTGTGCGCTCCTGCAAGTGCAGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR227339 representing NM_011577
Red=Cloning site Green=Tags(s)

MPPSGLRLLPLLLPLPWLLVLTPGRPAAGLSTCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPP
GPLPEAVLALYNSTRDRVAGESADPEPEPEADYYAKEVTRVLMVDRNNAIYEKTKDISHSIYMFFNTSDI
REAVPEPPLLSRAELRLQRLKSSVEQHVELYQKYSNNSWRYLGNRLLTPTDTPEWLSFDVTGVVRQWLNQ
GDGIQGFRFSAHCSCDSKDNKLHVEINGISPKRRGDLGTIHDMNRPFLLLMATPLERAQHLHSSRHRRAL
DTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGA
SASPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_011577
ORF Size 1170 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_011577.2
RefSeq Size 2094 bp
RefSeq ORF 1173 bp
Locus ID 21803
UniProt ID P04202
Cytogenetics 7 13.98 cM
MW 44.8 kDa
Gene Summary This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGF-beta family members. This encoded protein regulates cell proliferation, differentiation and growth, and can modulate expression and activation of other growth factors including interferon gamma and tumor necrosis factor alpha. Mice lacking a functional copy of this gene develop severe multifocal inflammatory disease, yolk sac defects and colon cancer. [provided by RefSeq, Aug 2016]

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