Gdf11 (NM_010272) Mouse Tagged ORF Clone
CAT#: MR223819
- TrueORF®
Gdf11 (Myc-DDK-tagged) - Mouse growth differentiation factor 11 (Gdf11)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_010272" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Gdf11 |
Synonyms | BMP-11; Bmp1; Bmp11 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR223819 representing NM_010272
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCTCGCGGCCCCGCTGCTGCTGGGCTTCCTGCTCCTCGCCCTGGAGCTGCGGCCCCGGGGGGAGG CGGCCGAGGGCCCCGCGGCGGCGGCGGCGGCAGCGGCGGCGGCGGCCGGGGTCGGGGGGGAGCGCTCGAG CCGGCCGGCCCCGTCCGCTCCGCCGGAGCCGGACGGCTGCCCCGTGTGCGTGTGGCGGCAGCACAGCCGC GAGCTGCGCCTAGAGAGCATCAAGTCGCAGATCCTGAGCAAACTGCGGCTCAAGGAGGCGCCCAACATCA GCCGGGAGGTAGTGAAGCAGCTGCTGCCCAAGGCGCCGCCGCTGCAGCAGATCCTGGATCTGCACGACTT CCAAGGCGACGCGCTGCAGCCTGAGGACTTCTTGGAAGAGGACGAGTACCACGCTACCACCGAGACGGTC ATAAGCATGGCCCAGGAGACGGACCCTGCAGTGCAGACAGATGGCAGCCCTCTCTGCTGTCATTTCCACT TCAGCCCCAAGGTGATGTTCACCAAGGTACTGAAGGCCCAACTGTGGGTGTACCTTCGGCCTGTGCCCCG CCCAGCCACAGTCTACCTGCAGATCTTACGACTGAAACCCCTAACTGGGGAAGGGACCGCAGGGGGAGGG GGTGGAGGCCGGCGTCACATCCGTATCCGTTCACTAAAGATTGAGCTACACTCACGTTCCGGCCACTGGC AGAGCATCGACTTCAAGCAAGTGCTACACAGCTGGTTTCGCCAGCCACAGAGCAACTGGGGAATCGAGAT CAACGCCTTTGATCCCAGCGGCACAGACCTGGCTGTCACCTCCCTGGGGCCAGGAGCTGAGGGGCTGCAT CCTTTCATGGAGCTTCGAGTCCTAGAGAACACGAAAAGGTCCCGGCGGAACCTAGGCCTGGACTGCGATG AACACTCGAGTGAGTCCCGCTGCTGCCGATATCCTCTCACAGTGGACTTTGAGGCTTTTGGCTGGGACTG GATCATCGCACCTAAGCGCTACAAGGCCAACTACTGCTCCGGCCAGTGCGAATACATGTTCATGCAAAAG TATCCACACACCCACTTGGTGCAACAGGCCAACCCAAGAGGCTCTGCTGGGCCCTGCTGCACCCCTACCA AGATGTCCCCAATCAACATGCTCTACTTCAATGACAAGCAGCAGATTATCTACGGCAAGATCCCTGGCAT GGTGGTGGATCGATGTGGCTGCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR223819 representing NM_010272
Red=Cloning site Green=Tags(s) MVLAAPLLLGFLLLALELRPRGEAAEGPAAAAAAAAAAAGVGGERSSRPAPSAPPEPDGCPVCVWRQHSR ELRLESIKSQILSKLRLKEAPNISREVVKQLLPKAPPLQQILDLHDFQGDALQPEDFLEEDEYHATTETV ISMAQETDPAVQTDGSPLCCHFHFSPKVMFTKVLKAQLWVYLRPVPRPATVYLQILRLKPLTGEGTAGGG GGGRRHIRIRSLKIELHSRSGHWQSIDFKQVLHSWFRQPQSNWGIEINAFDPSGTDLAVTSLGPGAEGLH PFMELRVLENTKRSRRNLGLDCDEHSSESRCCRYPLTVDFEAFGWDWIIAPKRYKANYCSGQCEYMFMQK YPHTHLVQQANPRGSAGPCCTPTKMSPINMLYFNDKQQIIYGKIPGMVVDRCGCS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_010272 |
ORF Size | 1215 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010272.2 |
RefSeq Size | 1811 bp |
RefSeq ORF | 1218 bp |
Locus ID | 14561 |
UniProt ID | Q9Z1W4 |
Cytogenetics | 10 D3 |
MW | 45.4 kDa |
Gene Summary | This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein plays a role in the development of the nervous and other organ systems, and may regulate aging. Mice lacking a functional copy of this gene exhibit impaired anteroposterior patterning and other developmental defects. [provided by RefSeq, Aug 2016] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC208537 | Gdf11 (untagged) - Mouse growth differentiation factor 11 (Gdf11), (10ug) |
USD 457.00 |
|
MG223819 | Gdf11 (tGFP-tagged) - Mouse growth differentiation factor 11 (Gdf11), (10ug) |
USD 657.00 |
|
MR223819L3 | Lenti ORF clone of Gdf11 (Myc-DDK-tagged) - Mouse growth differentiation factor 11 (Gdf11) |
USD 757.00 |
|
MR223819L4 | Lenti ORF clone of Gdf11 (mGFP-tagged) - Mouse growth differentiation factor 11 (Gdf11) |
USD 757.00 |
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