Barx1 (NM_007526) Mouse Tagged ORF Clone

USD 420.00


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In Stock

Size
    • 10 ug


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Specifications

Product Data
Product Name Barx1 (NM_007526) Mouse Tagged ORF Clone
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Barx1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>MR222169 representing NM_007526
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGCGGCCAGGGGAGCCGGGAGCCGCGCGCTTCGG[AT]CCGCCCGAGGGCTGTGCGGATCACCGAC
CGCATCGCTACCGCAGCTTCATGATCGAAGAGATCCTCACTGAGCCTCCGGGGCCCAAGGGCGCAGCGCC
CGCGGCCGCCGCTGCCGCCGCGGGCGAGCTGCTCAAGTTCGGCGTACAGGCGCTGCTGGCCGCCCGGCCC
TTCCACAGCCACCTGGCGGTGCTGAAGGCTGAGCAGGCCGCAGTGTTCAAGTTCCCACTGGCGCCGCTCG
GCTGCTCCGGGCTGGGCTCGGCGTTGTTGGCCGCCGGGCCTGGGATGCCCGGCCCCGCAGGCGCATCGCA
CCTGCCGCTGGAGCTGCAACTCCGCGGGAAGCTGGAAGCAGCTGGCTCTGGTGAACCTGGCGCGAAAGCC
AAGAAAGGACGCCGGAGTCGCACCGTATTCACTGAGCTGCAGCTGATGGGGCTGGAGAAACGCTTCGAGA
AGCAGAAGTACCTCTCTACCCCTGACAGAATAGATCTAGCTGAGTCCCTGGGCCTGAGCCAGTTACAGGT
GAAGACGTGGTATCAAAATCGGAGGATGAAATGGAAGAAAATAGTGCTGCAAGGTGGCGGCCTGGAGTCC
CCCACCAAGCCCAAGGGACGGCCCAAGAAGAACTCCATCCCCACGAGCGAGCAGCTCACGGAGCAAGAGC
GTGCCAAGGAGACAGAGAAGCCAGCGGAGACGCCGGGCGAGCCCAGCGACCGAAATTGCGAGGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR222169 representing NM_007526
Red=Cloning site Green=Tags(s)

MQRPGEPGAARFXXPPEGCADHRPHRYRSFMIEEILTEPPGPKGAAPAAAAAAAGELLKFGVQALLAARP
FHSHLAVLKAEQAAVFKFPLAPLGCSGLGSALLAAGPGMPGPAGASHLPLELQLRGKLEAAGSGEPGAKA
KKGRRSRTVFTELQLMGLEKRFEKQKYLSTPDRIDLAESLGLSQLQVKTWYQNRRMKWKKIVLQGGGLES
PTKPKGRPKKNSIPTSEQLTEQERAKETEKPAETPGEPSDRNCED

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_007526
ORF Size 765 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_007526.4, NP_031552.2
RefSeq Size 1366 bp
RefSeq ORF 765 bp
Locus ID 12022
Cytogenetics 13 24.9 cM
MW 27.7 kDa

Citations (1)

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