Ovgp1 (NM_007696) Mouse Tagged ORF Clone

CAT#: MR221693

Ovgp1 (Myc-DDK-tagged) - Mouse oviductal glycoprotein 1 (Ovgp1). Note: ORF is codon optimized

Product Images

Circular map for MR221693

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ovgp1
• Synonyms: 120kDa; AU016433; AU019448; Chit5; MOGP; muc9; OGP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR221693 representing NM_007696, codon optimized.
  Due to the complexity of NM_007696, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAGGCTGTTGCTCCTGGCTGGTCTGGTGCTCCTGATGAAGCATAGCGACGGGACAGCTTACAAGC
  TGGTGTGTTACTTCACCAATTGGGCTCATAGCAGGCCGGGGCCGGCATCTATAATGCCCCACGATCTTGA
  CCCTTTTTTGTGCACTCACCTGATCTTTGCCTTCGCCTCCATGAGCAACAATCAGATTGTGGCAAAGAAC
  CTGCAAGATGAGAACGTGCTTTATCCAGAGTTTAATAAGCTGAAGGAGAGAAACAGGGAGTTGAAAACAC
  TTCTGTCCATCGGGGGATGGAACTTTGGTACTAGCCGCTTCACAGCAATGCTGAGCACTCTGGCAAACAG
  GGAAAAATTTATTGACTCCGTGATCTCCTTCCTGCGCATCCATGGATTCGATGGATTGGATCTCTTTTTT
  CTGTACCCCGGATTGAGGGGCTCCCCCCCACATGACCGCTGGAATTTCCTCTTCCTGATCGAAGAGTTGC
  AATTCGCATTCGAACGCGAGGCCCTGCTGACACAGCATCCTAGGCTGTTGCTCAGCGCAGCTGTATCAGG
  CATCCCCAGTATTATACACACGTCTTACGATGCCCTGCTGCTGGGACGAAGGCTCGATTTCATTAACGTG
  CTGTCATACGACCTCCATGGCTCCTGGGAGAAGTTCACTGGACATAATTCCCCTTTGTTTTCACTGCCTG
  AAGACAGCAAGAGCAGCGCCTATGCCATGAACTACTGGCGGAAACTTGGCACACCTGCAGACAAACTGAT
  TATGGGCTTCCCAACCTACGGCCGGAACTTTTACCTCTTGAAGGAGAGCAAAAACGGACTTCAGACGGCT
  AGCATGGGTCCTGCATCCCCTGGAAAGTACACCAAACAGGCGGGCTTCCTTGCATATTACGAGGTGTGTT
  CCTTCGTGCAGCGCGCCAAAAAACACTGGATCGACTACCAGTATGTACCCTATGCGTTCAAGGGTAAAGA
  ATGGTTGGGCTATGATGACACTATCAGTTTTTCCTATAAAGCGATGTACGTGAAGAGGGAACATTTTGGC
  GGAGCCATGGTGTGGACGCTTGACATGGATGACGTGAGGGGAACCTTCTGCGGGAACGGCCCATTTCCAC
  TGGTGCACATACTCAACGAACTGCTGGTCCAGACCGAATCCAATTCAACCCCCCTCCCTCAGTTCTGGTT
  TACTTCCAGTGTCAACGCTTCAGGCCCCGGGTCTGAGAATACCGCGCTCACAGAGGTTCTGACTACCGAC
  ACCATTAAAATTTTGCCTCCCGGAGGTGAGGCAATGACCACTGAGGTGCATAGACGATACGAAAACATGA
  CCACAGTCCCATCAGATGGCTCCGTGACACCTGGAGGAACAGCCTCCCCACGCAAACACGCCGTGACTCC
  CGAGAATAATACTATGGCCGCCGAAGCAAAGACTATGTCAACACTGGATTTCTTCTCCAAGACGACAACT
  GGGGTGAGTAAAACAACTACAGGGATTTCAAAGACCACAACCGGGGTCAGTAAGACAACTACCGGAGTGT
  CCAAAGCAACCGCAGGCATTAGCAAGACAATACCCGAAATCTCAAAGGCAACCGCCGGGGTAAGCAAGAC
  CACTACAGGCGTATCTAAGACCACTACGGGCATTTCTAAGACTATAACCGGGGTGTCTAAAACTACAACC
  GGAATCTCTAAAACCACCACTGGCATAAGCAAAACTACTACGGGAGTGTCCAAGATTACTACTGGCGTCT
  CCAAAACTACTACAGGCATCAGTAAGACAACCACGGGCATCTCTCAGACAACTACTGGGATCAGCAAGAC
  AACTACAGATATCAGTAAGACGACCACCGGCATCTCCAAGACAACTCCCGGTATCTCAAAAACCACACCT
  GGAATGACAGTAATCGTTCAGACCCAGGCTAATGAGGCTGAGACAACCGCAACAATGGACCACCAGAGCG
  TGACGCCCACAGAGATGGATACAACCCTTTTTTATCTCAAGACCATGACCCCTAGTGAAAAGGAGACATC
  TCGGAAGAAAACTATGGTGCTTGAGAAAGCCACAGTTTCTCCGCGAGAAATGAGCGCTACCCCTAACGGG
  CAGTCCAAGACACTTAAGTGGGCCTCCCTGATTACCGAAGTTGAAACCTACAGCCAGGACGGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR221693 representing NM_007696
  Red=Cloning site Green=Tags(s)
  
  MGRLLLLAGLVLLMKHSDGTAYKLVCYFTNWAHSRPGPASIMPHDLDPFLCTHLIFAFASMSNNQIVAKN
  LQDENVLYPEFNKLKERNRELKTLLSIGGWNFGTSRFTAMLSTLANREKFIDSVISFLRIHGFDGLDLFF
  LYPGLRGSPPHDRWNFLFLIEELQFAFEREALLTQHPRLLLSAAVSGIPSIIHTSYDALLLGRRLDFINV
  LSYDLHGSWEKFTGHNSPLFSLPEDSKSSAYAMNYWRKLGTPADKLIMGFPTYGRNFYLLKESKNGLQTA
  SMGPASPGKYTKQAGFLAYYEVCSFVQRAKKHWIDYQYVPYAFKGKEWLGYDDTISFSYKAMYVKREHFG
  GAMVWTLDMDDVRGTFCGNGPFPLVHILNELLVQTESNSTPLPQFWFTSSVNASGPGSENTALTEVLTTD
  TIKILPPGGEAMTTEVHRRYENMTTVPSDGSVTPGGTASPRKHAVTPENNTMAAEAKTMSTLDFFSKTTT
  GVSKTTTGISKTTTGVSKTTTGVSKATAGISKTIPEISKATAGVSKTTTGVSKTTTGISKTITGVSKTTT
  GISKTTTGISKTTTGVSKITTGVSKTTTGISKTTTGISQTTTGISKTTTDISKTTTGISKTTPGISKTTP
  GMTVIVQTQANEAETTATMDHQSVTPTEMDTTLFYLKTMTPSEKETSRKKTMVLEKATVSPREMSATPNG
  QSKTLKWASLITEVETYSQDG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007696
• ORF Size: 2163 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007696.1, NM_007696.2, NP_031722.1
• RefSeq Size: 2525 bp
• RefSeq ORF: 2166 bp
• Locus ID: 12659
• UniProt ID: Q62010
• Cytogenetics: 3 F2.2
• MW: 78.8 kDa
• Gene Summary: Binds to oocyte zona pellucida in vivo. May play a role in the fertilization process and/or early embryonic development.[UniProtKB/Swiss-Prot Function]