Trem1 (NM_021406) Mouse Tagged ORF Clone
CAT#: MR219079
- TrueORF®
Trem1 (Myc-DDK-tagged) - Mouse triggering receptor expressed on myeloid cells 1 (Trem1)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_021406" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Trem1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR219079 representing NM_021406
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGAAGGCTGGGCTCTGGGGACTGCTGTGCGTGTTCTTTGTCTCAGAAGTCAAAGCTGCCATTGTTC TAGAGGAAGAAAGGTATGACCTAGTGGAGGGCCAGACTTTGACAGTGAAGTGTCCCTTCAACATCATGAA GTATGCCAACAGCCAGAAGGCTTGGCAGAGACTACCAGACGGGAAGGAACCCTTGACCCTGGTGGTCACA CAGAGGCCCTTTACAAGACCCAGTGAAGTCCACATGGGGAAGTTCACCCTGAAACATGACCCTAGTGAGG CCATGCTACAAGTTCAAATGACTGACCTTCAAGTGACAGACTCTGGATTGTATCGTTGTGTGATTTACCA TCCTCCGAATGACCCTGTTGTGCTCTTCCATCCTGTCCGCCTGGTGGTGACCAAGGGTTCTTCAGATGTG TTCACTCCTGTCATCATTCCTATTACAAGGCTGACAGAGCGTCCCATCCTTATTACCACAAAATACTCAC CCAGTGACACAACTACAACCCGATCCCTACCCAAGCCCACTGCGGTTGTTTCCTCTCCTGGTCTTGGAGT CACTATCATAAATGGGACAGATGCTGACAGTGTCTCCACATCCAGTGTTACTATTTCAGTCATCTGTGGA CTTCTCAGCAAGAGCCTGGTTTTCATCATCTTATTCATTGTCACAAAGAGGACATTTGGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR219079 representing NM_021406
Red=Cloning site Green=Tags(s) MRKAGLWGLLCVFFVSEVKAAIVLEEERYDLVEGQTLTVKCPFNIMKYANSQKAWQRLPDGKEPLTLVVT QRPFTRPSEVHMGKFTLKHDPSEAMLQVQMTDLQVTDSGLYRCVIYHPPNDPVVLFHPVRLVVTKGSSDV FTPVIIPITRLTERPILITTKYSPSDTTTTRSLPKPTAVVSSPGLGVTIINGTDADSVSTSSVTISVICG LLSKSLVFIILFIVTKRTFG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_021406 |
ORF Size | 690 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_021406.5 |
RefSeq Size | 3005 bp |
RefSeq ORF | 693 bp |
Locus ID | 58217 |
UniProt ID | Q9JKE2 |
Cytogenetics | 17 C |
MW | 25.9 kDa |
Gene Summary | Stimulates neutrophil and monocyte-mediated inflammatory responses. Triggers release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Amplifier of inflammatory responses that are triggered by bacterial and fungal infections and is a crucial mediator of septic shock (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC210167 | Trem1 (untagged) - Mouse triggering receptor expressed on myeloid cells 1 (Trem1), (10ug) |
USD 300.00 |
|
MG219079 | Trem1 (tGFP-tagged) - Mouse triggering receptor expressed on myeloid cells 1 (Trem1), (10ug) |
USD 500.00 |
|
MR219079L3 | Lenti ORF clone of Trem1 (Myc-DDK-tagged) - Mouse triggering receptor expressed on myeloid cells 1 (Trem1) |
USD 600.00 |
|
MR219079L4 | Lenti ORF clone of Trem1 (mGFP-tagged) - Mouse triggering receptor expressed on myeloid cells 1 (Trem1) |
USD 600.00 |
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