Dgka (NM_016811) Mouse Tagged ORF Clone

CAT#: MR210336

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  • TrueORF®

Dgka (Myc-DDK-tagged) - Mouse diacylglycerol kinase, alpha (Dgka)


  View "NM_016811" in other vectors (6)

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Size
    • 10 ug


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Specifications

Product Data
Product Name Dgka (NM_016811) Mouse Tagged ORF Clone
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Dgka
Synonyms 80kDa; AW146112; Dagk1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>MR210336 representing NM_016811
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCAAAGAGAAGGGCCTCATCAGCCCAGAAGACTTCGCTCAGCTGCAAAAGTACATAGAATACTCCA
CCAAAAGGGTCAGCGATGTACTGAAGGTCTTTGACGATGGTGAGATGAACAGATTTTGCCAGGGAGATGC
CATTGGGTACCTAGGATTTGAACAATTCATGAAAATGTATCTGGAAATGGAGGAGGTTCCCCATCACCTA
TGCTGGGCTCTGTTTTGGTCCTTCCATACTAGTCAAGTTGCGGCTGAGAAGACCAAGTCAAAAGCCAATG
TGATCTGTCTCAGTGATGTGTACTGCTACTTCACCCTCCTAGAAGGTGGCCGGCCAGAAGACAAGCTAGA
GTTCACCTTCAAGCTATATGACATGGACAGAAATGGGATCCTGGATAGCACGGAAGTGGAAAAAATCATC
CTTCAGATGATGCGAGTGGCCGAATATCTAGACTGGGATGTGTCTGAGCTGAGACCGATCCTTCAGGAGA
TGATGAGAGAGATGGATCAGGACGGCAGTGGCTCTGTGTCTCTAGACGAGTGGGTCCGTGCTGGGGCTAC
CACAGTGCCACTGCTTGTGCTCCTGGGGATGGACGTGACTATGAAAGATGATGGCAACCATATATGGAGA
CCCAAGAGATTCACCAGACTGGTCTACTGCAACCTGTGCGAACAGAGCATTAGCCTTGGCAAACAGGGCC
TGAGCTGTAACTTCTGTAAGTACATTGTTCATGACCACTGTGCCATGAAGGCCCAGCCTTGTGAAGTCAG
CACCTATGCCAAGTCTCGGAAAGACATTGGTGTCCAGTCACACTTATGGGTTCGAGGAGGCTGTCATTCC
GGGCGTTGTGACCGCTGCCAGAAAAAGATCCGGACCTACCACAGCCTAACAGGACTGCACTGTGTGTGGT
GCCACCTGGAGATCCATGATGACTGTCTGCAGGCTGTAGGTCCTGAGTGTGACTGTGGGTTGCTCCGTGA
TCATATCCTGCCTCCGTGTTCCATCTACCCCAGCGTCCTGGTATCTGGACAAGAGTGCAAACACAAGACC
ACAGATGATACAAGCCTGTGCACCCCTGAGGCTTTTCGGATTGAACCCGTTTCTAACACCCACCCCCTTC
TAGTCTTCATCAATCTTAAGAGCGGAGGCAAGCAGGGGCAGAGTGTGCTTTGGAAATTTCAGTACATTCT
GAACCCGCGGCAGGTGTTCGACCTGAAGGATGGTCCGGAGCCAGGGCTCAGATTCTTTAAAGATGTTCCT
CAGTTCCGGATATTGGTGTGTGGTGGAGATGGCACAGTAGGCTGGGTTCTAGAGACCATTGACAAAGCCA
ACTTTGCCACTGTGCCTCCCGTTGCTGTGCTGCCCCTGGGCACTGGAAATGATCTGGCTCGGTGCCTAAG
ATGGGGAAGAGGTTATGAAGGTGAGAATTTGAGAAAGATTCTCAAGGATATAGAGCTAAGTAAGGTGGTA
TATCTCGACCGATGGTTCCTGGAAGTGATTCCCCAACAAAATGGAGAAAAGAGTGATCCAGTTCCCTCTC
AAATCATCAATAACTACTTCTCCATTGGCGTGGATGCTTCTATCGCTCACCGATTCCATCTCATGAGAGA
GAAATATCCTGAGAAGTTCAATAGCAGAATGAAGAACAAGCTTTGGTACTTTGAGTTTGCCACATCTGAG
TCCATCTTCTCAACATGCAAAAAGCTGGAAGAGTCTGTAACCGTTGAGATATGCGGGAAGCTGCTGGATC
TGAGCGACCTATCCCTAGAAGGCATTGCGGTATTGAACATCCCGAGCACGCACGGTGGCTCCAACCTCTG
GGGTGACACCAAGAGACCTCATGGGGATACATGTGAGATCAACCAGGCATTGGGCAGTGCGGCCAAAATA
ATCACAGACCCCGATATCCTGAAGACCTGTGTGCCAGACATGAGTGACAAGCGGCTGGAAGTGGTAGGAA
TAGAGGGTGCAATTGAGATGGGCCAGATCTATACCAGGCTCAAGAGTGCTGGACACCGGCTGGCCAAGTG
CTCCGAGATCACATTCCAGACCACAAAAACCCTCCCCATGCAAATTGACGGGGAGCCCTGGATGCAGGCA
CCCTGTACAATCAAGATCACCCATAAGAACCAGATGCCTATGCTTATGGGTCCACCTTCCAACTCCTACA
ATTTCTTTGGCTTTTGGAGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR210336 representing NM_016811
Red=Cloning site Green=Tags(s)

MAKEKGLISPEDFAQLQKYIEYSTKRVSDVLKVFDDGEMNRFCQGDAIGYLGFEQFMKMYLEMEEVPHHL
CWALFWSFHTSQVAAEKTKSKANVICLSDVYCYFTLLEGGRPEDKLEFTFKLYDMDRNGILDSTEVEKII
LQMMRVAEYLDWDVSELRPILQEMMREMDQDGSGSVSLDEWVRAGATTVPLLVLLGMDVTMKDDGNHIWR
PKRFTRLVYCNLCEQSISLGKQGLSCNFCKYIVHDHCAMKAQPCEVSTYAKSRKDIGVQSHLWVRGGCHS
GRCDRCQKKIRTYHSLTGLHCVWCHLEIHDDCLQAVGPECDCGLLRDHILPPCSIYPSVLVSGQECKHKT
TDDTSLCTPEAFRIEPVSNTHPLLVFINLKSGGKQGQSVLWKFQYILNPRQVFDLKDGPEPGLRFFKDVP
QFRILVCGGDGTVGWVLETIDKANFATVPPVAVLPLGTGNDLARCLRWGRGYEGENLRKILKDIELSKVV
YLDRWFLEVIPQQNGEKSDPVPSQIINNYFSIGVDASIAHRFHLMREKYPEKFNSRMKNKLWYFEFATSE
SIFSTCKKLEESVTVEICGKLLDLSDLSLEGIAVLNIPSTHGGSNLWGDTKRPHGDTCEINQALGSAAKI
ITDPDILKTCVPDMSDKRLEVVGIEGAIEMGQIYTRLKSAGHRLAKCSEITFQTTKTLPMQIDGEPWMQA
PCTIKITHKNQMPMLMGPPSNSYNFFGFWS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_016811
ORF Size 2190 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reference Data
RefSeq NM_016811.1, NM_016811.2, NP_058091.2
RefSeq Size 2842
RefSeq ORF 2193
Locus ID 13139
Cytogenetics 10 77.14 cM
MW 82.8 kDa

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68 Mouse Clones