Arntl (NM_007489) Mouse Tagged ORF Clone

CAT#: MR209553

Arntl (Myc-DDK-tagged) - Mouse aryl hydrocarbon receptor nuclear translocator-like (Arntl)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Arntl
• Synonyms: Arnt3; bHLHe5; Bmal1; BMAL1b; bmal1b'; MOP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209553 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGACCAGAGAATGGACATTTCCTCAACCATCAGCGACTTCATGTCTCCGGGCCCCACCGACCTAC
  TCTCCGGTTCCCTGGGCACCAGTGGTGTGGACTGCAATCGCAAGAGGAAAGGCAGTGCCACTGACTACCA
  AGAAAGTATGGACACAGACAAAGATGACCCTCATGGAAGGTTAGAATATGCAGAACACCAAGGAAGGATC
  AAGAATGCAAGGGAGGCCCACAGTCAGATTGAAAAGAGGCGTCGGGACAAAATGAACAGTTTCATTGATG
  AATTGGCTTCTTTGGTACCAACATGCAATGCAATGTCCAGGAAGTTAGATAAACTCACCGTGCTAAGGAT
  GGCTGTTCAGCACATGAAAACTTTGAGAGGTGCCACCAACCCATACACAGAAGCAAACTACAAGCCAACA
  TTTCTATCAGATGACGAACTGAAACACCTAATTCTCAGGGCAGCAGATGGATTTTTGTTTGTCGTAGGAT
  GTGACCGAGGGAAGATCCTCTTTGTCTCCGAGTCTGTCTTCAAGATCCTCAATTATAGCCAGAATGACCT
  TATTGGCCAGAGCTTGTTTGACTACCTGCATCCAAAAGATATTGCCAAAGTTAAGGAACAGCTATCTTCC
  TCGGACACTGCGCCCCGGGAGCGACTCATTGATGCCAAGACTGGACTTCCGGTTAAAACGGATATAACCC
  CTGGGCCCTCCCGGCTATGCTCTGGAGCCCGCCGCTCTTTCTTCTGTAGAATGAAGTGCAACAGGCCTTC
  AGTAAAGGTGGAAGATAAGGACTTCGCCTCTACCTGTTCAAAGAAAAAAGCAGATCGAAAAAGCTTCTGC
  ACAATCCACAGCACAGGCTATTTGAAAAGCTGGCCACCCACGAAGATGGGGCTGGACGAAGACAATGAGC
  CAGACAACGAGGGCTGCAACCTCAGCTGCCTCGTTGCAATCGGGCGCCTGCACTCGCACATGGTTCCACA
  ACCAGCGAACGGGGAAATACGGGTGAAATCTATGGAGTACGTTTCTCGACACGCAATAGATGGGAAATTT
  GTTTTTGTAGATCAGAGGGCGACAGCTATTTTGGCGTATCTACCACAGGAACTTCTAGGTACATCATGTT
  ATGAGTATTTTCATCAAGACGACATAGGACACCTCGCAGAATGTCACAGGCAAGTTTTACAGACAAGAGA
  AAAGATCACAACTAATTGCTATAAGTTTAAGATCAAAGATGGTTCTTTTATCACGCTACGAAGTCGATGG
  TTCAGTTTCATGAACCCGTGGACCAAGGAAGTTGAATACATTGTCTCAACCAACACTGTTGTTTTAGCCA
  ATGTCCTGGAAGGCGGGGACCCAACCTTCCCGCAGCTAACAGCACCCCCCCACAGCATGGACAGCATGCT
  GCCCTCTGGAGAAGGTGGCCCAAAGAGGACTCATCCCACTGTCCCAGGCATTCCAGGGGGAACCAGAGCC
  GGAGCAGGAAAAATAGGTCGAATGATCGCGGAGGAAATCATGGAAATCCACAGGATAAGAGGGTCATCGC
  CTTCCAGCTGTGGCTCCAGCCCGCTGAACATCACAAGTACGCCTCCCCCTGATGCCTCTTCTCCAGGAGG
  CAAGAAGATTCTAAATGGAGGGACTCCAGACATTCCTTCCACTGGACTATTACCAGGGCAGGCTCAGGAG
  ACCCCAGGGTATCCCTATTCTGATAGTTCTTCTATTCTTGGTGAGAACCCCCACATAGGCATCGATATGA
  TAGATAACGACCAAGGATCAAGTAGTCCCAGTAACGATGAGGCAGCAATGGCTGTCATCATGAGCCTCTT
  GGAAGCAGATGCGGGGCTGGGTGGCCCCGTTGACTTTAGTGACTTGCCATGGCCGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209553 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MADQRMDISSTISDFMSPGPTDLLSGSLGTSGVDCNRKRKGSATDYQESMDTDKDDPHGRLEYAEHQGRI
  KNAREAHSQIEKRRRDKMNSFIDELASLVPTCNAMSRKLDKLTVLRMAVQHMKTLRGATNPYTEANYKPT
  FLSDDELKHLILRAADGFLFVVGCDRGKILFVSESVFKILNYSQNDLIGQSLFDYLHPKDIAKVKEQLSS
  SDTAPRERLIDAKTGLPVKTDITPGPSRLCSGARRSFFCRMKCNRPSVKVEDKDFASTCSKKKADRKSFC
  TIHSTGYLKSWPPTKMGLDEDNEPDNEGCNLSCLVAIGRLHSHMVPQPANGEIRVKSMEYVSRHAIDGKF
  VFVDQRATAILAYLPQELLGTSCYEYFHQDDIGHLAECHRQVLQTREKITTNCYKFKIKDGSFITLRSRW
  FSFMNPWTKEVEYIVSTNTVVLANVLEGGDPTFPQLTAPPHSMDSMLPSGEGGPKRTHPTVPGIPGGTRA
  GAGKIGRMIAEEIMEIHRIRGSSPSSCGSSPLNITSTPPPDASSPGGKKILNGGTPDIPSTGLLPGQAQE
  TPGYPYSDSSSILGENPHIGIDMIDNDQGSSSPSNDEAAMAVIMSLLEADAGLGGPVDFSDLPWPL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007489
• ORF Size: 1881 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007489.4
• RefSeq Size: 2921 bp
• RefSeq ORF: 1881 bp
• Locus ID: 11865
• UniProt ID: Q9WTL8
• Cytogenetics: 7 59.17 cM
• MW: 68.7 kDa
• Gene Summary: The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with Clock. This heterodimer binds E-box enhancer elements upstream of Period (Per1, Per2, Per3) and Cryptochrome (Cry1, Cry2) genes and activates transcription of these genes. Per and Cry proteins heterodimerize and repress their own transcription by interacting in a feedback loop with Clock/Arntl complexes. Defects in this gene have been linked to infertility, problems with gluconeogenesis and lipogenesis, and altered sleep patterns. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2014]