Arntl (NM_007489) Mouse Tagged ORF Clone

CAT#: MR209553

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Arntl (Myc-DDK-tagged) - Mouse aryl hydrocarbon receptor nuclear translocator-like (Arntl)



  "NM_007489" in other vectors (4)

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USD 580.00


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Size
    • 10 ug


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Specifications

Product Data
Product Name Arntl (NM_007489) Mouse Tagged ORF Clone
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Arntl
Synonyms Arnt3; bHLHe5; Bmal1; BMAL1b; bmal1b'; MOP3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>MR209553 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGACCAGAGAATGGACATTTCCTCAACCATCAGCGACTTCATGTCTCCGGGCCCCACCGACCTAC
TCTCCGGTTCCCTGGGCACCAGTGGTGTGGACTGCAATCGCAAGAGGAAAGGCAGTGCCACTGACTACCA
AGAAAGTATGGACACAGACAAAGATGACCCTCATGGAAGGTTAGAATATGCAGAACACCAAGGAAGGATC
AAGAATGCAAGGGAGGCCCACAGTCAGATTGAAAAGAGGCGTCGGGACAAAATGAACAGTTTCATTGATG
AATTGGCTTCTTTGGTACCAACATGCAATGCAATGTCCAGGAAGTTAGATAAACTCACCGTGCTAAGGAT
GGCTGTTCAGCACATGAAAACTTTGAGAGGTGCCACCAACCCATACACAGAAGCAAACTACAAGCCAACA
TTTCTATCAGATGACGAACTGAAACACCTAATTCTCAGGGCAGCAGATGGATTTTTGTTTGTCGTAGGAT
GTGACCGAGGGAAGATCCTCTTTGTCTCCGAGTCTGTCTTCAAGATCCTCAATTATAGCCAGAATGACCT
TATTGGCCAGAGCTTGTTTGACTACCTGCATCCAAAAGATATTGCCAAAGTTAAGGAACAGCTATCTTCC
TCGGACACTGCGCCCCGGGAGCGACTCATTGATGCCAAGACTGGACTTCCGGTTAAAACGGATATAACCC
CTGGGCCCTCCCGGCTATGCTCTGGAGCCCGCCGCTCTTTCTTCTGTAGAATGAAGTGCAACAGGCCTTC
AGTAAAGGTGGAAGATAAGGACTTCGCCTCTACCTGTTCAAAGAAAAAAGCAGATCGAAAAAGCTTCTGC
ACAATCCACAGCACAGGCTATTTGAAAAGCTGGCCACCCACGAAGATGGGGCTGGACGAAGACAATGAGC
CAGACAACGAGGGCTGCAACCTCAGCTGCCTCGTTGCAATCGGGCGCCTGCACTCGCACATGGTTCCACA
ACCAGCGAACGGGGAAATACGGGTGAAATCTATGGAGTACGTTTCTCGACACGCAATAGATGGGAAATTT
GTTTTTGTAGATCAGAGGGCGACAGCTATTTTGGCGTATCTACCACAGGAACTTCTAGGTACATCATGTT
ATGAGTATTTTCATCAAGACGACATAGGACACCTCGCAGAATGTCACAGGCAAGTTTTACAGACAAGAGA
AAAGATCACAACTAATTGCTATAAGTTTAAGATCAAAGATGGTTCTTTTATCACGCTACGAAGTCGATGG
TTCAGTTTCATGAACCCGTGGACCAAGGAAGTTGAATACATTGTCTCAACCAACACTGTTGTTTTAGCCA
ATGTCCTGGAAGGCGGGGACCCAACCTTCCCGCAGCTAACAGCACCCCCCCACAGCATGGACAGCATGCT
GCCCTCTGGAGAAGGTGGCCCAAAGAGGACTCATCCCACTGTCCCAGGCATTCCAGGGGGAACCAGAGCC
GGAGCAGGAAAAATAGGTCGAATGATCGCGGAGGAAATCATGGAAATCCACAGGATAAGAGGGTCATCGC
CTTCCAGCTGTGGCTCCAGCCCGCTGAACATCACAAGTACGCCTCCCCCTGATGCCTCTTCTCCAGGAGG
CAAGAAGATTCTAAATGGAGGGACTCCAGACATTCCTTCCACTGGACTATTACCAGGGCAGGCTCAGGAG
ACCCCAGGGTATCCCTATTCTGATAGTTCTTCTATTCTTGGTGAGAACCCCCACATAGGCATCGATATGA
TAGATAACGACCAAGGATCAAGTAGTCCCAGTAACGATGAGGCAGCAATGGCTGTCATCATGAGCCTCTT
GGAAGCAGATGCGGGGCTGGGTGGCCCCGTTGACTTTAGTGACTTGCCATGGCCGCTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR209553 protein sequence
Red=Cloning site Green=Tags(s)

MADQRMDISSTISDFMSPGPTDLLSGSLGTSGVDCNRKRKGSATDYQESMDTDKDDPHGRLEYAEHQGRI
KNAREAHSQIEKRRRDKMNSFIDELASLVPTCNAMSRKLDKLTVLRMAVQHMKTLRGATNPYTEANYKPT
FLSDDELKHLILRAADGFLFVVGCDRGKILFVSESVFKILNYSQNDLIGQSLFDYLHPKDIAKVKEQLSS
SDTAPRERLIDAKTGLPVKTDITPGPSRLCSGARRSFFCRMKCNRPSVKVEDKDFASTCSKKKADRKSFC
TIHSTGYLKSWPPTKMGLDEDNEPDNEGCNLSCLVAIGRLHSHMVPQPANGEIRVKSMEYVSRHAIDGKF
VFVDQRATAILAYLPQELLGTSCYEYFHQDDIGHLAECHRQVLQTREKITTNCYKFKIKDGSFITLRSRW
FSFMNPWTKEVEYIVSTNTVVLANVLEGGDPTFPQLTAPPHSMDSMLPSGEGGPKRTHPTVPGIPGGTRA
GAGKIGRMIAEEIMEIHRIRGSSPSSCGSSPLNITSTPPPDASSPGGKKILNGGTPDIPSTGLLPGQAQE
TPGYPYSDSSSILGENPHIGIDMIDNDQGSSSPSNDEAAMAVIMSLLEADAGLGGPVDFSDLPWPL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_007489
ORF Size 1881 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_007489.1, NM_007489.2, NM_007489.3, NM_007489.4, NP_031515.1
RefSeq Size 2921 bp
RefSeq ORF 1881 bp
Locus ID 11865
Cytogenetics 7 59.17 cM
MW 68.7 kDa
Gene Summary The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with Clock. This heterodimer binds E-box enhancer elements upstream of Period (Per1, Per2, Per3) and Cryptochrome (Cry1, Cry2) genes and activates transcription of these genes. Per and Cry proteins heterodimerize and repress their own transcription by interacting in a feedback loop with Clock/Arntl complexes. Defects in this gene have been linked to infertility, problems with gluconeogenesis and lipogenesis, and altered sleep patterns. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2014]

Citations (1)

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