Ttc5 (NM_001080949) Mouse Tagged ORF Clone

CAT#: MR207023

Ttc5 (Myc-DDK-tagged) - Mouse tetratricopeptide repeat domain 5 (Ttc5), transcript variant 1

Product Images

Circular map for MR207023

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ttc5
• Synonyms: 5930437N14; AW743060
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207023 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGCTGATGAAGAGGAAGAAGCGAAGCACGTCTTGCAGAAATTGCAGGGACTGGTGGATCGGCTCT
  ACTGTTTTCGAGACAGTTACTTTGAGACACATAGTGTCGAAGATGCAGGACGGAAGCAGCAGGATGTACA
  GGAAGAGATGGAGAAGACCCTGCAGCAGATGGAGGAAGTACTCGGTTCTGCCCAGGTTGAGGCACAGGCT
  CTGATGCTGAAGGGGAAGGCACTGAATGTGACTCCTGATTATAGCCCTGAGGCCGAGGTGCTTCTCTCCA
  AGGCCGTGAAGCTGGAGCCTGAGCTGGTGGAAGCCTGGAACCAGCTGGGTGAGGTGTACTGGAAGAAAGG
  AGATGTCACATCTGCCCACACCTGCTTCTCAGGAGCCCTCACCCACTGCAAGAACAAAGTCTCTCTGCAG
  AACTTGTCCATGGTGCTCCGCCAGCTGCAGACCGACTCTGGAGATGAACATTCTCGCCACGTCATGGACA
  GCGTCCGGCAGGCTAAGTTGGCCGTGCAGATGGATGTCCTTGATGGCCGCTCCTGGTATATCCTGGGGAA
  TGCATATCTTTCTCTTTATTTCAATACTGGCCAGAACCCTAAGATCTCCCAGCAAGCCCTCAGTGCCTAT
  GCTCAAGCAGAGAAGGTGGACAGGAAAGCATCTAGCAACCCTGACCTTCATCTCAACAGGGCGACGTTAC
  ATAAATATGAGGAGAGTTATGGGGAGGCCCTTGAGGGTTTCTCTCAGGCTGCAGCGCTGGACCCTGCGTG
  GCCAGAGCCCCAGCAACGAGAACAGCAACTCTTGGAATTCCTCAGTAGGCTAACCAGCCTCCTGGAGAGC
  AAGGGGAAGACAAAGCCCAAGAAGCTGCAGAGCATGCTGGGAAGCTTGCGCCCAGCTCATCTGGGCCCCT
  GTGGTGATGGGCGCTATCAGTCGGCCTCTGGGCAGAAGATGACCCTGGAGCTTAAGCCACTGAGCACCCT
  GCAGCCTGGTGTGAACAGTGGCACCGTGGTCCTGGGAAAGGTGGTGTTCAGCCTGACCACAGAGGAGAAA
  GTCCCCTTCACGTTTGGCTTGGTAGATTCGGATGGTCCCTGCTATGCAGTGATGGTGTATAATGTGGTGC
  AGAGCTGGGGAGTGCTCATCGGGGACTCTGTAGCTATTCCTGAGCCCAACCTTCGGCATCATCAAATCCG
  GCACAAGGGAAAGGACTATTCCTTCTCCAGCGTGCGTGTGGAAACGCCTCTTCTGCTGGTGGTGAATGGA
  AAGCCACAGAACTCCAGCAGTCAAGCCTCTGCCACAGTAGCTTCAAGGCCACAGTGTGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207023 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MMADEEEEAKHVLQKLQGLVDRLYCFRDSYFETHSVEDAGRKQQDVQEEMEKTLQQMEEVLGSAQVEAQA
  LMLKGKALNVTPDYSPEAEVLLSKAVKLEPELVEAWNQLGEVYWKKGDVTSAHTCFSGALTHCKNKVSLQ
  NLSMVLRQLQTDSGDEHSRHVMDSVRQAKLAVQMDVLDGRSWYILGNAYLSLYFNTGQNPKISQQALSAY
  AQAEKVDRKASSNPDLHLNRATLHKYEESYGEALEGFSQAAALDPAWPEPQQREQQLLEFLSRLTSLLES
  KGKTKPKKLQSMLGSLRPAHLGPCGDGRYQSASGQKMTLELKPLSTLQPGVNSGTVVLGKVVFSLTTEEK
  VPFTFGLVDSDGPCYAVMVYNVVQSWGVLIGDSVAIPEPNLRHHQIRHKGKDYSFSSVRVETPLLLVVNG
  KPQNSSSQASATVASRPQCE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001080949
• ORF Size: 1323 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001080949.1, NM_001080949.2, NP_001074418.1
• RefSeq Size: 1849 bp
• RefSeq ORF: 1323 bp
• Locus ID: 219022
• UniProt ID: Q99LG4
• Cytogenetics: 14 C1
• MW: 48.8 kDa
• Gene Summary: Adapter protein involved in p53/TP53 response that acts by regulating and mediating the assembly of multi-protein complexes. Required to facilitate the interaction between JMY and p300/EP300 and increase p53/TP53-dependent transcription and apoptosis. Prevents p53/TP53 degradation by MDM2.[UniProtKB/Swiss-Prot Function]