Polr1d (NM_181730) Mouse Tagged ORF Clone
CAT#: MR200671
- TrueORF®
Polr1d (Myc-DDK-tagged) - Mouse polymerase (RNA) I polypeptide D (Polr1d), transcript variant 2
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_181730" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Polr1d |
Synonyms | 16kD; 1110003G10Rik; AC19; mRP; RPA16; Rpo; Rpo1-3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR200671 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGACGATCAGGAGCTGGAGAGGAAAGCCATAGAGGAATTGCTAAAGGAGGCAAAGCGTGGGAAAA CCAGAGCGGAGACAATGGGGCCGATGGGCTGGATGAAGTGTCCTCTTGCTGGTACAAATAAAAGATTCCT AATTAACACAATTAAGAACACACTGCCCTCCCATAAAGAGCAAGACCATGAACAAAAAGAGGGCAGTAAG GAGCCTGGCAAAAGCCAAGACCAGAAGGAAGCAAGTGGGAAGAAATACCGAAGTCACTCGTACAAGCGCA GCCTTCACTCGTCCCGGGGCTCCGCAGGCTGCTCTCCTCCACGGAAGCGGACCTCCCGGACCTCCGGGGA CAAGTGCGACCCACGGCCCAGCAGGCGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR200671 protein sequence
Red=Cloning site Green=Tags(s) MEDDQELERKAIEELLKEAKRGKTRAETMGPMGWMKCPLAGTNKRFLINTIKNTLPSHKEQDHEQKEGSK EPGKSQDQKEASGKKYRSHSYKRSLHSSRGSAGCSPPRKRTSRTSGDKCDPRPSRR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_181730 |
ORF Size | 381 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_181730.1, NM_181730.2, NM_181730.3, NM_181730.4, NP_859419.1 |
RefSeq Size | 1147 bp |
RefSeq ORF | 381 bp |
Locus ID | 20018 |
Cytogenetics | 5 G3 |
MW | 14.3 kDa |
Gene Summary | This gene encodes an RNA polymerase subunit that is a component of both the RNA polymerase I and RNA polymerase III complexes. RNA polymerase I is associated with transcription of pre-ribosomal RNAs, while RNA polymerase III is associated with transcription of small RNAs. Pseudogenes of this gene have been defined on chromosomes 4 and 6. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2013] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC201795 | Polr1d (untagged) - Mouse polymerase (RNA) I polypeptide D (Polr1d), transcript variant 2, (10ug) |
USD 150.00 |
|
MG200671 | Polr1d (tGFP-tagged) - Mouse RNA polymerase 1-3 (Rpo1-3), transcript variant 2 |
USD 350.00 |
|
MR200671L3 | Lenti ORF clone of Polr1d (Myc-DDK-tagged) - Mouse polymerase (RNA) I polypeptide D (Polr1d), transcript variant 2 |
USD 450.00 |
|
MR200671L4 | Lenti ORF clone of Polr1d (mGFP-tagged) - Mouse polymerase (RNA) I polypeptide D (Polr1d), transcript variant 2 |
USD 450.00 |
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