E2f3 (NM_010093) Mouse Tagged ORF Clone
CAT#: MG222646
- TrueORF®
E2f3 (tGFP-tagged) - Mouse E2F transcription factor 3 (E2f3), (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_010093" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | E2f3 |
Synonyms | E2f3a; E2F3b |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG222646 representing NM_010093
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAAAGGGGATCCAGCCCGCCCTGGAGCAGTACCTGGTGACCGCCGGGGGTGGGGAGGGGGCGGCTG TCGTCGCCGCCGCCGCTGCAGCCTCCATGGACAAAAGGGCACTGCTAGCCAGCCCCGGCTTCGCCGCCGC CGCCGCCCCGGGCACGTACATCCAGATCCTCACTACGAACCCTTCCACCACGTCCTGTGCCACCTCCCTC CAAAGTGGCGCCCTGACCGCCGGCCCCCTTCTCCCCAGTGTCCCCGGCACGGAGCCGGCCGCCAGCAGCC TCTACACCACGCCACAAGGACCCTCCAGCAGAGTCGGGCTGCTGCAGCAGCCACCAGCACCAGGACGCGG CGGCGGCGGTGGCCCACCGGCAAAGCGAAGGCTGGAACTGGGCGAGAGTGGCCATCAGTACCTCTCAGAT GGTCTAAAGACCCCCAAGGGCAAAGGAAGAGCTGCACTACGGAGTCCCGATAGTCCAAAAACTCCAAAAT CTCCCTCAGAAAAAACGCGGTATGATACGTCCCTCGGTCTGCTCACCAAGAAGTTCATTCAGCTCCTGAG CCAGTCTCCTGATGGGGTCCTGGATCTGAACAAGGCAGCAGAGGTGCTCAAGGTGCAGAAGAGGAGGATT TACGACATCACCAACGTGCTGGAAGGCATCCACCTCATTAAGAAGAAGTCTAAGAACAACGTCCAGTGGA TGGGCTGCAGTCTGTCTGAGGATGGGGGCATGCTGGCCCAGTGTCAAGGCCTGTCCAAAGAAGTGACTGA GCTCAGTCAGGAAGAGAAGAAATTAGATGAGCTGATCCAAAGCTGTACCCTGGACCTCAAACTGTTAACC GAGGATTCAGAGAATCAAAGGTTAGCTTATGTTACATATCAAGATATTCGAAAAATTAGTGGCCTTAAAG ACCAAACTGTTATAGTTGTGAAAGCCCCTCCAGAAACGAGACTTGAAGTGCCTGACTCAATAGAGAGCCT ACAAATCCATTTGGCAAGTACCCAAGGGCCCATTGAGGTTTACTTGTGTCCAGAAGAGACGGAAACACAC AGACCCATGAAAACAAATAACCAAGACCACAATGGGAATATCCCCAAGCCCACTTCCAAAGACTTGGCTT CTAACAACTCAGGACATAGTGACTGCTCGGTTTCTACAGCAAACCTCTCTCCTCTGGCCTCCCCAGCCAA CCTTTTACAGCAGACTGAGGACCAAATCCCGTCCAACCTTGAAGGACCTTTTGTGAACTTACTGCCTCCC CTGCTCCAAGAGGACTACCTGCTGAGCCTGGGGGAGGAAGAGGGCATCAGTGACCTCTTCGATGCCTATG ATTTGGAAAAGCTGCCTCTGGTGGAGGACTTCATGTGTAGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222646 representing NM_010093
Red=Cloning site Green=Tags(s) MRKGIQPALEQYLVTAGGGEGAAVVAAAAAASMDKRALLASPGFAAAAAPGTYIQILTTNPSTTSCATSL QSGALTAGPLLPSVPGTEPAASSLYTTPQGPSSRVGLLQQPPAPGRGGGGGPPAKRRLELGESGHQYLSD GLKTPKGKGRAALRSPDSPKTPKSPSEKTRYDTSLGLLTKKFIQLLSQSPDGVLDLNKAAEVLKVQKRRI YDITNVLEGIHLIKKKSKNNVQWMGCSLSEDGGMLAQCQGLSKEVTELSQEEKKLDELIQSCTLDLKLLT EDSENQRLAYVTYQDIRKISGLKDQTVIVVKAPPETRLEVPDSIESLQIHLASTQGPIEVYLCPEETETH RPMKTNNQDHNGNIPKPTSKDLASNNSGHSDCSVSTANLSPLASPANLLQQTEDQIPSNLEGPFVNLLPP LLQEDYLLSLGEEEGISDLFDAYDLEKLPLVEDFMCS TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_010093 |
ORF Size | 1371 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010093.3, NP_034223.1 |
RefSeq Size | 5106 bp |
RefSeq ORF | 1374 bp |
Locus ID | 13557 |
UniProt ID | O35261 |
Cytogenetics | 13 A3.2 |
Gene Summary | Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner. Inhibits adipogenesis, probably through the repression of CEBPA binding to its target gene promoters (PubMed:20176812).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC216107 | E2f3 (untagged) - Mouse E2F transcription factor 3 (E2f3), (10ug) |
USD 686.00 |
|
MR222646 | E2f3 (Myc-DDK-tagged) - Mouse E2F transcription factor 3 (E2f3) |
USD 686.00 |
|
MR222646L3 | Lenti ORF clone of E2f3 (Myc-DDK-tagged) - Mouse E2F transcription factor 3 (E2f3) |
USD 986.00 |
|
MR222646L4 | Lenti ORF clone of E2f3 (mGFP-tagged) - Mouse E2F transcription factor 3 (E2f3) |
USD 986.00 |
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