E2f3 (NM_010093) Mouse Untagged Clone

CAT#: MC216107

E2f3 (untagged) - Mouse E2F transcription factor 3 (E2f3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: E2f3
• Synonyms: E2f3a; E2F3b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216107 representing NM_010093
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAAAGGGGATCCAGCCCGCCCTGGAGCAGTACCTGGTGACCGCCGGGGGTGGGGAGGGGGCGGCTG
  TCGTCGCCGCCGCCGCTGCAGCCTCCATGGACAAAAGGGCACTGCTAGCCAGCCCCGGCTTCGCCGCCGC
  CGCCGCCCCGGGCACGTACATCCAGATCCTCACTACGAACCCTTCCACCACGTCCTGTGCCACCTCCCTC
  CAAAGTGGCGCCCTGACCGCCGGCCCCCTTCTCCCCAGTGTCCCCGGCACGGAGCCGGCCGCCAGCAGCC
  TCTACACCACGCCACAAGGACCCTCCAGCAGAGTCGGGCTGCTGCAGCAGCCACCAGCACCAGGACGCGG
  CGGCGGCGGTGGCCCACCGGCAAAGCGAAGGCTGGAACTGGGCGAGAGTGGCCATCAGTACCTCTCAGAT
  GGTCTAAAGACCCCCAAGGGCAAAGGAAGAGCTGCACTACGGAGTCCCGATAGTCCAAAAACTCCAAAAT
  CTCCCTCAGAAAAAACGCGGTATGATACGTCCCTCGGTCTGCTCACCAAGAAGTTCATTCAGCTCCTGAG
  CCAGTCTCCTGATGGGGTCCTGGATCTGAACAAGGCAGCAGAGGTGCTCAAGGTGCAGAAGAGGAGGATT
  TACGACATCACCAACGTGCTGGAAGGCATCCACCTCATTAAGAAGAAGTCTAAGAACAACGTCCAGTGGA
  TGGGCTGCAGTCTGTCTGAGGATGGGGGCATGCTGGCCCAGTGTCAAGGCCTGTCCAAAGAAGTGACTGA
  GCTCAGTCAGGAAGAGAAGAAATTAGATGAGCTGATCCAAAGCTGTACCCTGGACCTCAAACTGTTAACC
  GAGGATTCAGAGAATCAAAGGTTAGCTTATGTTACATATCAAGATATTCGAAAAATTAGTGGCCTTAAAG
  ACCAAACTGTTATAGTTGTGAAAGCCCCTCCAGAAACGAGACTTGAAGTGCCTGACTCAATAGAGAGCCT
  ACAAATCCATTTGGCAAGTACCCAAGGGCCCATTGAGGTTTACTTGTGTCCAGAAGAGACGGAAACACAC
  AGACCCATGAAAACAAATAACCAAGACCACAATGGGAATATCCCCAAGCCCACTTCCAAAGACTTGGCTT
  CTAACAACTCAGGACATAGTGACTGCTCGGTTTCTACAGCAAACCTCTCTCCTCTGGCCTCCCCAGCCAA
  CCTTTTACAGCAGACTGAGGACCAAATCCCGTCCAACCTTGAAGGACCTTTTGTGAACTTACTGCCTCCC
  CTGCTCCAAGAGGACTACCTGCTGAGCCTGGGGGAGGAAGAGGGCATCAGTGACCTCTTCGATGCCTATG
  ATTTGGAAAAGCTGCCTCTGGTGGAGGACTTCATGTGTAGTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010093
• Insert Size: 1374 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010093.3, NP_034223.1
• RefSeq Size: 5106 bp
• RefSeq ORF: 1374 bp
• Locus ID: 13557
• UniProt ID: O35261
• Cytogenetics: 13 A3.2
• Gene Summary: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner. Inhibits adipogenesis, probably through the repression of CEBPA binding to its target gene promoters (PubMed:20176812).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1, also known as E2f3a) represents the longer transcript and encodes the longer isoform (E2f3a).