Ak4 (NM_001177605) Mouse Tagged ORF Clone
CAT#: MG221763
- TrueORF®
Ak4 (tGFP-tagged) - Mouse adenylate kinase 4 (Ak4) nuclear gene encoding mitochondrial protein transcript variant 3, (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_001177605" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Ak4 |
Synonyms | Ak-3; Ak-4; Ak3; Ak3l1; AK 4; D4Ertd274e |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG221763 representing NM_001177605
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCCAAACTCCTGCGCGCGGTCATCCTCGGGCCGCCCGGCTCTGGCAAGGGCACCGTTTGCGAAA GGATCGCCCAGAACTTTGGCCTCCAGCATCTCTCCAGCGGCCACTTGTTGCGGGAGAACCTCAAGACCGG CACGGAAGTTGGTGACGTGGCAAAACAGTACCTAGAAAAAGGTCTTTTGGTTCCAGATCACGTGATCACA CGCCTAATGATGTCAGAACTGGAGACTCGGAGTGCCCAGCACTGGCTGTTAGATGGATTCCCGAGGACAT TAGTACAGGCAGAAGCCCTGGACGGAATCTGTGATGTGGACCTAGTAATCAGTTTGAATATTCCTTTTGA GACACTTAAAGATCGTCTGAGCCGACGGTGGATTCACCCTTCTAGCGGGAGGGTCTATAACCTGGACTTC AACCCACCTCAAGTGCAGGGGATTGATGACATCACTGGTGAGCCACTTGTCCAACAGGAAGATGATAAAC CTGAAGCAGTTGCTGCCAGGCTAAGACGGTACAAGGATGCGGCAAAACCAGTCATCGAACTGTACAAGAG CCGTGGAGTGCTCCACCAGTTTTCTGGGACGGAGACTAACAGAATCTGGCCTTATGTTTACACACTTTTC TCCAACAAGATCACACCTATTCAATCCAAAGAAGCCTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG221763 representing NM_001177605
Red=Cloning site Green=Tags(s) MASKLLRAVILGPPGSGKGTVCERIAQNFGLQHLSSGHLLRENLKTGTEVGDVAKQYLEKGLLVPDHVIT RLMMSELETRSAQHWLLDGFPRTLVQAEALDGICDVDLVISLNIPFETLKDRLSRRWIHPSSGRVYNLDF NPPQVQGIDDITGEPLVQQEDDKPEAVAARLRRYKDAAKPVIELYKSRGVLHQFSGTETNRIWPYVYTLF SNKITPIQSKEAY TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001177605 |
ORF Size | 669 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001177605.1, NP_001171076.1 |
RefSeq Size | 4927 bp |
RefSeq ORF | 672 bp |
Locus ID | 11639 |
UniProt ID | Q9WUR9 |
Cytogenetics | 4 46.84 cM |
Gene Summary | Involved in maintaining the homeostasis of cellular nucleotides by catalyzing the interconversion of nucleoside phosphates. Efficiently phosphorylates AMP and dAMP using ATP as phosphate donor, but phosphorylates only AMP when using GTP as phosphate donor. Also displays broad nucleoside diphosphate kinase activity.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC208122 | Ak4 (untagged) - Mouse adenylate kinase 4 (Ak4), nuclear gene encoding mitochondrial protein, transcript variant 3, (10ug) |
USD 330.00 |
|
MR221763 | Ak4 (Myc-DDK-tagged) - Mouse adenylate kinase 4 (Ak4), nuclear gene encoding mitochondrial protein, transcript variant 3 |
USD 300.00 |
|
MR221763L3 | Lenti ORF clone of Ak4 (Myc-DDK-tagged) - Mouse adenylate kinase 4 (Ak4), nuclear gene encoding mitochondrial protein, transcript variant 3 |
USD 600.00 |
|
MR221763L4 | Lenti ORF clone of Ak4 (mGFP-tagged) - Mouse adenylate kinase 4 (Ak4), nuclear gene encoding mitochondrial protein, transcript variant 3 |
USD 600.00 |
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