Nlrx1 (NM_178420) Mouse Tagged ORF Clone

CAT#: MG211358

Nlrx1 (tGFP-tagged) - Mouse NLR family member X1 (Nlrx1)

Product Images

Circular map for MG211358

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nlrx1
• Synonyms: BC034204; NOD9
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211358 representing NM_178420
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGTGGGGCTGCCATTTGCCCAGGACCTCTTGGGGCTCTGGCCTGGGAAGAACACCCCAGCTACCAG
  ATGAGCATATCTCCTTCTTGATCCAGTGGAGCTGGCCCTTTAAAGGGGTGCATCCCCTGAGGCCCCCTAG
  GGCCTTTATCCGTTACCATGGAAACTCGGCAGACAGTGCTCCCCCACCAGGGAGGCATGGGCAGCTGTTC
  AGGAGCATCTCTGCCACAGAAGCTATCCAAAGGCATCGCCGGAACCTCACCGAGTGGTTTAGCCGACTGC
  CCAGAGAGGAGCGCCAGTTTGGACCAACCTTTGCTCTAGACACAGTTCATGTTGACCCCGTGATCCGAGA
  GAGCACCCCAGATGAGCTGCTTCGCCCGTCCACGGAGCTGGCCACGGGGCATCAGCAAACCCAGGCAGGG
  CTCCCCCCACTGGCCCTGTCTCAGCTTTTTGACCCGGATTCTTGTGGGCGCCGCGTGCAGACCGTGGTGT
  TGTATGGGACCGTGGGTACTGGCAAGAGCACGTTGGTACGGAAGATGGTCTTAGACTGGTGTTACGGGAG
  ACTGCCTGCCTTTGAGCTTCTCATCCCCTTCTCCTGTGAGGACTTGTCATCCCTGGGCTCCACCCCAGCT
  TCCCTGTGCCAACTTGTGACCCAGCGTTACACACCCCTGAAAGAGGTGTTGCCCCTGATGACTGCTGCGG
  GATCCCGCCTGCTCTTTGTGCTCCATGGCTTGGAGCGCCTCAACCTTGACTTCCGGCTGGCAGGCACAGG
  GCTTTGCAGTGACCCGGAGGAACCCGGGCCACCAGCTGCCATCATAGTCAACCTGCTGCGCAAATACATG
  CTTCCCGAGGCCAGCATTCTGGTAACCACCCGGCCTTCCACCATTAGCCGAATCCCTAGCAAGTATGTGG
  GCCGCTATGGTGAGATCTGTGGCTTCTCTGATACCAACCTGCAGAAGCTCTACTTCCAGCTCCGCCTTAA
  CCAGCCTGACTGTGGGTACGGTGCTGGGGGTGCCAGTGTCTCAGTCACACCAGCTCAGCGCGACAACCTG
  ATTCAAATGCTCTCCCGGAACCTGGAGGGGCACCACCAGATTGCCGCAGCCTGCTTTCTGCCTTCCTATT
  GCTGGCTTGTCTGTGCTACTTTGCACTTCCTGCATGCTCCCACACCTGCTGGTCAGACCCTCACAAGCAT
  CTATACCAGCTTTCTACGCCTGAACTTCAGTGGGGAAACACTGGACAGCACCCACACGTCCAATCTATCC
  CTGATGTCCTATGCAGCCCGGACTATGGGCAAGCTGGCCTACGAGGGCGTGCCATCCCGAAAGACCTACT
  TCTCTGAAGAGGATGTCCGTGGCTGCCTGGAAGCTGGCATCAAGACAGAGGAAGAGTTTCAACTGCTTCA
  GATCTTCCGCAGGGACGCCCTGAGGTTTTTCCTGGCCCCGTGTGTGGAACCAGGGCACCTGGGTACCTTC
  GTGTTCACCGTGCCCGCCATGCAGGAGTATCTGGCTGCCCTCTACAGCGTGCTTGGTTTGCGCAAGACAG
  CCCTGCAGCGGGTGGGCAAAGAAGTGGTTGAATTTGTGGGCCGTGTTGGGGAAGATGTCAGCCTGGTATT
  GGGCATTGTGGCCAAGCTGTTGCCCCTGCGGATTCTGCCTCTGCTCTTCAACTTGCTCAAGGTAGTTCCG
  CGAGTGTTTGGGCGCATGGTGAGTAAGAGCCGGGAGGCAGTGGCCCAGGCCATGGTGCTGGAGATGTTCC
  GGGAGGAAGACTACTACAATGACGATGTTCTGGATCAGATGGGTGCCAGCATCCTGGGTGTGGAGGGCCC
  CCGGCGCCACCCAGATGAACCCTCTGAGGATGAAGTCTTTGAGCTCTTCCCCATGTTCATGGGCGGACTT
  CTCTCTGCCCACAACCGGGCGGTGCTGGCTCAGCTTGGCTGTCCCATCAAGAACCTGGATGCCCTGGAGA
  ATGCCCAGGCCATCAAGAAGAAGCTGGGGAAGCTGGGTCGGCAGGTGCTGCCCCCCTCGGAGCTTCTTGA
  CCATCTCTTCTTCCACTATGAGTTCCAGAACCAGCGCTTCTCAGCTGAGGTGCTGGGCTCCCTACGCCAG
  CTCAATTTAGCAGGGGTGCGCATGACACCCCTCAAGTGCACAGTGGTAGCCTCTGTACTGGGAAGTGGAA
  GGCACCCCCTGGATGAGGTGAACTTGGCCTCCTGCCAGCTGGATCCCGCTGGGCTACACACTCTCATGCC
  TGTCCTCCTGCGTGCCCGGAAACTGGGGTTGCAACTCAACAATCTGGGCCCCGAGGCCTGCAGAGACCTC
  CGAGACCTGCTCTTACACGATCAATGCCAGATCACCACTCTTAGGCTCTCCAACAACCCACTGACAGCAG
  CTGGTGTGGGCTTACTGATGGACGGGCTGGCAGGAAACACTTCGGTGACACACCTGTCTCTGCTGCACAC
  TGACCTTGGAGACGAGGGACTGGAACTGCTGGCTGCCCAGCTGGACCGAAACAAACAACTGCAGGAGCTG
  AACGTGGCCTACAACGGTGCTGGTGACACAGTGGCTCTGGCCTTGGCTAAGGCTGCTCGGGAGCACCCTT
  CCCTGGAGCTGCTGCACCTCTACTTCAATGAGCTGAGTTCAGAGGGCCGCCAGGTCCTGCGGGATTTGGG
  GGGCTCTGGTGAAGGTGGTGCCCGGGTCGTAGCCTCGCTGACAGAAGGGACGGCGGTGTCTGAGTACTGG
  TCAGTGATCCTTAGTGAAGTCCAGCGCAACGTCCACAGCTGGGACCCGCTCCGGGTCCAGAGGCATCTCA
  AGCTGCTGCTCCGTGATCTGGAGGACAGCCGGGGCGCCACCCTTAATCCCTGGCGCAAGGCTCAGCTTCT
  GCGAGTGGAGGGCGAGGTCAAGACTCTTCTGGAGCAGCTGGGAGGTTCTGGACAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211358 representing NM_178420
  Red=Cloning site Green=Tags(s)
  
  MRWGCHLPRTSWGSGLGRTPQLPDEHISFLIQWSWPFKGVHPLRPPRAFIRYHGNSADSAPPPGRHGQLF
  RSISATEAIQRHRRNLTEWFSRLPREERQFGPTFALDTVHVDPVIRESTPDELLRPSTELATGHQQTQAG
  LPPLALSQLFDPDSCGRRVQTVVLYGTVGTGKSTLVRKMVLDWCYGRLPAFELLIPFSCEDLSSLGSTPA
  SLCQLVTQRYTPLKEVLPLMTAAGSRLLFVLHGLERLNLDFRLAGTGLCSDPEEPGPPAAIIVNLLRKYM
  LPEASILVTTRPSTISRIPSKYVGRYGEICGFSDTNLQKLYFQLRLNQPDCGYGAGGASVSVTPAQRDNL
  IQMLSRNLEGHHQIAAACFLPSYCWLVCATLHFLHAPTPAGQTLTSIYTSFLRLNFSGETLDSTHTSNLS
  LMSYAARTMGKLAYEGVPSRKTYFSEEDVRGCLEAGIKTEEEFQLLQIFRRDALRFFLAPCVEPGHLGTF
  VFTVPAMQEYLAALYSVLGLRKTALQRVGKEVVEFVGRVGEDVSLVLGIVAKLLPLRILPLLFNLLKVVP
  RVFGRMVSKSREAVAQAMVLEMFREEDYYNDDVLDQMGASILGVEGPRRHPDEPSEDEVFELFPMFMGGL
  LSAHNRAVLAQLGCPIKNLDALENAQAIKKKLGKLGRQVLPPSELLDHLFFHYEFQNQRFSAEVLGSLRQ
  LNLAGVRMTPLKCTVVASVLGSGRHPLDEVNLASCQLDPAGLHTLMPVLLRARKLGLQLNNLGPEACRDL
  RDLLLHDQCQITTLRLSNNPLTAAGVGLLMDGLAGNTSVTHLSLLHTDLGDEGLELLAAQLDRNKQLQEL
  NVAYNGAGDTVALALAKAAREHPSLELLHLYFNELSSEGRQVLRDLGGSGEGGARVVASLTEGTAVSEYW
  SVILSEVQRNVHSWDPLRVQRHLKLLLRDLEDSRGATLNPWRKAQLLRVEGEVKTLLEQLGGSGH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178420
• ORF Size: 2925 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178420.2
• RefSeq Size: 3657 bp
• RefSeq ORF: 2928 bp
• Locus ID: 270151
• UniProt ID: Q3TL44
• Cytogenetics: 9 A5.2
• Gene Summary: Participates in antiviral signaling.[UniProtKB/Swiss-Prot Function]