Shkbp1 (NM_138676) Mouse Tagged ORF Clone

CAT#: MG210149

Shkbp1 (GFP-tagged) - Mouse Sh3kbp1 binding protein 1 (Shkbp1)

Specifications

Product Data

• Product Name: Shkbp1 (NM_138676) Mouse Tagged ORF Clone
• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Shkbp1
• Synonyms: B930062H15Rik; Sb1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210149 representing NM_138676
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGTCGCGACGACTGCAGTGGAGGGGGTCCCGAGTCGGGGAGCACCCGGGGAAGTGATTCATCTGA
  ACGTAGGGGGCAAGCGGTTCAGTACCTCTCGCCAGACGCTTACCTGGATCCCAGACTCCTTCTTTTCCAG
  TCTTTTGAGCGGACGCATCTCAACCCTGAAAGATGAGACCGGAGCTATCTTCATCGACCGGGACCCCACC
  GTCTTTGCCCCCATCCTTAATTTCCTGCGGACCAAAGAGTTAGATCCCAGGGGTGTCCATGGTTCCAGCC
  TCCTCCATGAAGCCCAATTCTACGGACTCACTCCACTGGTTCGTCGCCTGCAGGTGAGGGAAGAGTTGGA
  TCGATCTTCCTGCGGAAATGTCCTCTTCAATGGTTATCTACCTCCTCCAGTGTTCCCAGTGAAGCGGCGA
  AACCGGCACAGCCTGGTGGGGCCCCAGCAAATAGGGGGACGCCCAGCCCCTGTTCGACGGAGCAACACGA
  TGCCCCCCAACTTGGGTAATGCAGGGCTGCTGGGCCGAATGTTAGATGAGAGAGCACCTCCATCCCCATC
  AGGCCAACCAGAGGAGCCAGGGATGGTGCGCCTGGTGTGTGGTCACCACAACTGGATTGCTGTGGCATAT
  ACCCACTTTCTCGTCTGTTATAGGTTGAAGGAAGCCTCGGGCTGGCAGCTTGCATTCTCCAGCCCGCGCC
  TGGACTGGCCCATCGAGCGCCTGGCTCTTGCGGCTCGGGTGCTTGGCGGTGCTCCGGGTGAGCATGACAA
  GATGGTGGCTGCAGCTACTGGCAGCGAGATCCTTCTGTGGGCCCTACAAGCACAAGGTGGTGGCTCTGAG
  ATAGGTGTCTTCCACCTGGGTGTGCCAGTGGAGGCCTTGTTCTTTGTTGGGAATCAGCTCATTGCCACAA
  GTCACACAGGGCGCATCGGTGTCTGGAACGCTGTGACCAAGCACTGGCAGGTCCAGGAGGTCCAGCCTAT
  CACGAGCTATGATGCAGCCGGCTCCTTCCTCCTCCTGGGCTGCAGCAATGGCTCCATCTATTATGTAGAT
  GTGCAGAAATTCCCCCTGCGGATGAAGGACAACGATCTCCTTGTCAGTGAGCTCTACCGAGACCCAGCAG
  AGGATGGGGTCACCGCCCTCAGCGTCTACCTCACCCCCAAGACCAGTGACAGTGGGAACTGGATTGAGAT
  TGCCTATGGCACCAGCTCCGGGGTGGTGCGGGTCATTGTGCAGCACCCTGAGACGGTGGGCTCGGGACCC
  CAGCTCTTCCAGACCTTCAGTGTGCACCGCAGCCCGGTCACTAAGATCATGCTGTCAGAGAAGCATCTTA
  TCTCTGTGTGTGCCGACAACAACCATGTTCGGACGTGGTCTGTGACCCGCTTTCGAGGCATGATCTCCAC
  CCAGCCTGGTTCCACTCCGCTGGCATCCTTCAAGATCCTGGCGCTGGAATCTGTTGATGGGCTCGGTGGC
  TGCAGTGCTGGCAATGACATTGGTCCCTATGGTGAACGGGATGACCAGCAGGTGTTCATCCAGAAAGTGG
  TGCCAAATGCCAGTCAGCTATTTGTGCGTCTCTCTTCCACTGGGCAGCGGGTGTGTTCTGTGCGCTCGGT
  GGATGGGTCTCCCACGACTGCCTTCACAGTGTTGGAGTGTGAGGGCTCCAGGCGGCTGGGCTCCAGACCC
  CGACGTTACCTGCTCACTGGTCAGGCCAACGGCAGCCTGGCCATGTGGGACCTGACCACTGCCATGGATG
  GCCTGGGCCAAACTCCTGCAGGCGGCCTGACAGAAGAAGAGCTGATGGACCAACTGGAGCAATGTGAGCT
  GAGCCCATTGGCTTCCTCTCGGGGCTCTTTCCCCAGCCCCTCACCCAGGACCTCTCTTACCAGTCTCCAC
  TCAGCCTCCAGCAACACCTCCCTGTATGGCCCCAGAGGGAGCCCCAGCCCACCACAGGCAGAGGCCCGTC
  GCCGCGGGGCAGGAAGCTTCGTGGATCGGTGTCAGGAACTGGCAAGGGGTGCGCCAGAGCTCAGATGGCC
  GCCAACACCAGCCCCTCGACCCTCCACCAGTCTTGGGAATCCCCTGACAGCTCTCAAGAAAACTCTTAAC
  GAAACTTCCTTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210149 representing NM_138676
  Red=Cloning site Green=Tags(s)
  
  MAVATTAVEGVPSRGAPGEVIHLNVGGKRFSTSRQTLTWIPDSFFSSLLSGRISTLKDETGAIFIDRDPT
  VFAPILNFLRTKELDPRGVHGSSLLHEAQFYGLTPLVRRLQVREELDRSSCGNVLFNGYLPPPVFPVKRR
  NRHSLVGPQQIGGRPAPVRRSNTMPPNLGNAGLLGRMLDERAPPSPSGQPEEPGMVRLVCGHHNWIAVAY
  THFLVCYRLKEASGWQLAFSSPRLDWPIERLALAARVLGGAPGEHDKMVAAATGSEILLWALQAQGGGSE
  IGVFHLGVPVEALFFVGNQLIATSHTGRIGVWNAVTKHWQVQEVQPITSYDAAGSFLLLGCSNGSIYYVD
  VQKFPLRMKDNDLLVSELYRDPAEDGVTALSVYLTPKTSDSGNWIEIAYGTSSGVVRVIVQHPETVGSGP
  QLFQTFSVHRSPVTKIMLSEKHLISVCADNNHVRTWSVTRFRGMISTQPGSTPLASFKILALESVDGLGG
  CSAGNDIGPYGERDDQQVFIQKVVPNASQLFVRLSSTGQRVCSVRSVDGSPTTAFTVLECEGSRRLGSRP
  RRYLLTGQANGSLAMWDLTTAMDGLGQTPAGGLTEEELMDQLEQCELSPLASSRGSFPSPSPRTSLTSLH
  SASSNTSLYGPRGSPSPPQAEARRRGAGSFVDRCQELARGAPELRWPPTPAPRPSTSLGNPLTALKKTLN
  ETSF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138676
• ORF Size: 2112 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138676.2, NP_619617.2
• RefSeq Size: 2347 bp
• RefSeq ORF: 2115 bp
• Locus ID: 192192
• Cytogenetics: 7 A3
• Gene Summary: Inhibits CBL-SH3KBP1 complex mediated down-regulation of EGFR signaling by sequestration of SH3KBP1. Binds to SH3KBP1 and prevents its interaction with CBL and inhibits translocation of SH3KBP1 to EGFR containing vesicles upon EGF stimulation (PubMed:21830225).[UniProtKB/Swiss-Prot Function]