Pogk (BC072587) Mouse Tagged ORF Clone

CAT#: MG209555

Pogk (tGFP-tagged) - Mouse pogo transposable element with KRAB domain (cDNA clone MGC:99912 IMAGE:6829796)

Product Images

Circular map for MG209555

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pogk
• Synonyms: BASS2, mKIAA1513
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209555 representing BC072587
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGGCCACAGCCTTCGTCGCCCTTTGCGGCGACGCCGCCCGCTCGGCCGGGAAATGGAGTCCCGGG
  CCTACCCTCTGAATTTGACCCTGAAAGAAGAGCAAAAGGAGGAAGAGGTTGAGATCCAAGAGTTGGAGGA
  TGGCCCCATAGATATGCAGAAAGTACAAATCTGCTCAGAAGGCGCATGGGTACCAGCTCTATTTGATGAG
  GTGGCCATATATTTCTCAGATGAGGAGTGGGAAGTTCTGACAGAGCAACAAAAAGCCCTCTACCGGGAAG
  TCATGAGGATGAATTATGAGACTGTCCTGTCCCTGGAATTCCCATTCCCCAAGCCAGATATGATCAATCG
  GTTGGAAAGGGACGAGGAGTGTCCTAATTCTGATGAGTGGCGGCTCCAGGGAGTAACCTTTGCAGAAAAT
  GAAGAATCTGACTTTAGGACTCCAGATTGGGCAAGCCCGACGAATGCCACCTCCCATTTTCCTCAACCTC
  AGCCCTTCAACAGCTTTGGCCTGCGTCTGCCTCAGGACATCACTGAACTGCCTGAGTGGACTGAGGGCTA
  CCCCTTCTATATGGCCATGGGCTTCCCCGGGTATGACCTCTCAGCTGACGACTTGGCGAGCAAGTTTCAG
  TTCAGTCGTGGCATGCGCCGCAGTTATGATGCAGGGTTCAAGCTGATGGTGGTGGAGTACGCCGAGAGCA
  CCAACAACTGTCAGGCTGCCAAGCAGTTTGGGGTGTTGGAAAAAAATGTTCGAGACTGGCGCAAAGTGAA
  GCCACAGCTCCAAAATGCTCATGCCATGCGGCGGGCATTCCGAGGCCCCAAGAATGGAAGGTTTGCTCTG
  GTAGACCAGCGAGTGGCTGAGTATGTCAGATACATGCAGGCCAAAGGGGACCCTATCACTAGGGAGGCAA
  TGCAGTTGAAAGCTCTTGAAATCGCCCAGGAAATGAACATTCCAGAGAAAGGGTTCAAGGCAAGTCTGGG
  CTGGTGTCGAAGAATGATGAGAAGGTATGACCTGTCTCTGAGGCATAAAGTGCCAGTCCCTCAACACCTG
  GCCGAGGACCTGACTGAAAAGCTTGTCACTTACCAGCAGAGTGTGCTGGCTCTGCGCAGGACACACGACT
  ATGAAGTGGCCCAGATGGGCAATGCAGACGAGACGCCCATCTGCTTAGAAGTCCCCTCTAGAGTGACTGT
  TGACAACCAGGGCGAAAAGCCCATCTTGGTCAAGACACCAGGCAGGGAGAAATTGAGGATCACAGCCATG
  CTGGGGGTCTTGGCTGATGGGAGGAAGTTACCACCGTACATCATTTTGAGGGGAACATACATCCCCCCTG
  GGAAGTTTCCCAGTGGCATGGAGATCCGCTGCCACCGCTACGGATGGATGACTGAGGACTTGATGCAAGA
  CTGGTTGGAAGTTGTGTGGAGACGGAGAACTGGAGCTGTGCCCAGACAGCGAGGGATGCTGATCCTGAAT
  GGCTTCCGGTGCCATGCCACTGACTCTGTGAAGAGTTCCATGGAGAACATGAACACGGACATGGTGATCA
  TCCCAGGGGGCCTGACCTCCCAGCTGCAGGTGCTGGATGTCGTTGTCTACAAGCCTCTGAACGACAGCGT
  TCGGGCCCAGTATTCCAACTGGCTTCTGGCTGGGAACCTGGCACTCAGCCCCACTGTAAATGCTAAGAAG
  CCCCCCCTGGGCCTCTTCCTGGAGTGGATCATGGTTGCATGGAACAGCATCTCAAGTGAATCAATTGTCC
  AGGGGTTCAGAAAGTGCCACATCTCCAGCAACTTAGAGGACGAAGGTGACGTCCTCTGGGAAATCGAGGG
  CGAGTTGCCCAAAGAACCGCCAAAAGAATGTGGTCCTGAAAGTGTGGCTGAGGGTGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209555 representing BC072587
  Red=Cloning site Green=Tags(s)
  
  MPGHSLRRPLRRRRPLGREMESRAYPLNLTLKEEQKEEEVEIQELEDGPIDMQKVQICSEGAWVPALFDE
  VAIYFSDEEWEVLTEQQKALYREVMRMNYETVLSLEFPFPKPDMINRLERDEECPNSDEWRLQGVTFAEN
  EESDFRTPDWASPTNATSHFPQPQPFNSFGLRLPQDITELPEWTEGYPFYMAMGFPGYDLSADDLASKFQ
  FSRGMRRSYDAGFKLMVVEYAESTNNCQAAKQFGVLEKNVRDWRKVKPQLQNAHAMRRAFRGPKNGRFAL
  VDQRVAEYVRYMQAKGDPITREAMQLKALEIAQEMNIPEKGFKASLGWCRRMMRRYDLSLRHKVPVPQHL
  AEDLTEKLVTYQQSVLALRRTHDYEVAQMGNADETPICLEVPSRVTVDNQGEKPILVKTPGREKLRITAM
  LGVLADGRKLPPYIILRGTYIPPGKFPSGMEIRCHRYGWMTEDLMQDWLEVVWRRRTGAVPRQRGMLILN
  GFRCHATDSVKSSMENMNTDMVIIPGGLTSQLQVLDVVVYKPLNDSVRAQYSNWLLAGNLALSPTVNAKK
  PPLGLFLEWIMVAWNSISSESIVQGFRKCHISSNLEDEGDVLWEIEGELPKEPPKECGPESVAEGD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC072587
• ORF Size: 1880 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC072587, AAH72587
• RefSeq Size: 2451 bp
• RefSeq ORF: 1880 bp
• Locus ID: 71592
• Cytogenetics: 1 H2.3