B3galt6 (BC082998) Mouse Tagged ORF Clone

CAT#: MG204731

B3galt6 (tGFP-tagged) - Mouse UDP-Gal:betaGal beta 1,3-galactosyltransferase, polypeptide 6 (cDNA clone MGC:91010 IMAGE:5011578), complete

Product Images

Circular map for MG204731

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: B3galt6
• Synonyms: BB129894; GalTII
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204731 representing BC082998
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGTATTCCGGCGCGCTTGGCGGCACCGGGTGGCGCTGGGCCTAGGCGGCCTGGCGTTCTGCGGCA
  CCACTCTGTTGTACCTGGCGCGCTGCGCTTCCGAGGGCGAGACGCCCTCCGCTTCCGGAGCCGCTCGGCC
  CCGCGCTAAGGCCTTCCTGGCGGTGCTGGTGGCCAGTGCGCCCCGCGCGGTCGAGCGCCGCACCGCAGTG
  CGCAGCACGTGGCTGGCACCGGAGAGGCGTGGCGGACCCGAGGACGTGTGGGCGCGCTTCGCCGTGGGCA
  CTGGCGGCTTAGGCTCGGAGGAGCGGCGCGCTCTTGAGCTCGAGCAGGCGCAGCACGGGGACCTACTGCT
  GCTGCCCGCCCTGCGCGACGCCTACGAGAACCTCACGGCCAAGGTCCTGGCCATGCTGACCTGGCTGGAT
  GAGCGCGTGGACTTCGAGTTCGTGCTCAAGGCGGACGACGACTCCTTTGCGCGCCTGGACGCTATCCTGG
  TGGACCTACGCGCACGGGAGCCCGCACGCCGCCGGCGCCTCTACTGGGGCTTCTTTTCCGGGCGCGGGCG
  CGTCAAGCCGGGAGGTCGCTGGCGAGAAGCAGCCTGGCAACTCTGCGACTACTACCTGCCCTACGCGCTG
  GGCGGTGGCTATGTCCTTTCTGCGGACCTGGTGCATTACCTGCGCCTCAGCCGCGAATACCTGCGCGCGT
  GGCACAGTGAAGACGTATCGCTGGGCACCTGGCTGGCACCAGTGGATGTGCAACGGGAGCATGACCCACG
  CTTCGACACGGAGTACAAATCTCGAGGCTGCAACAATCAGTATCTGGTGACACACAAGCAAAGCCCAGAG
  GACATGTTGGAGAAGCAACAGATGTTGCTGCATGAGGGCCGGTTGTGCAAGCATGAGGTGCAGTTGCGCC
  TTTCCTATGTCTATGACTGGTCAGCTCCACCCTCCCAGTGCTGCCAGCGCAAGGAGGGCGTTCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204731 representing BC082998
  Red=Cloning site Green=Tags(s)
  
  MKVFRRAWRHRVALGLGGLAFCGTTLLYLARCASEGETPSASGAARPRAKAFLAVLVASAPRAVERRTAV
  RSTWLAPERRGGPEDVWARFAVGTGGLGSEERRALELEQAQHGDLLLLPALRDAYENLTAKVLAMLTWLD
  ERVDFEFVLKADDDSFARLDAILVDLRAREPARRRRLYWGFFSGRGRVKPGGRWREAAWQLCDYYLPYAL
  GGGYVLSADLVHYLRLSREYLRAWHSEDVSLGTWLAPVDVQREHDPRFDTEYKSRGCNNQYLVTHKQSPE
  DMLEKQQMLLHEGRLCKHEVQLRLSYVYDWSAPPSQCCQRKEGVP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC082998
• ORF Size: 977 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC082998, AAH82998
• RefSeq Size: 1599 bp
• RefSeq ORF: 977 bp
• Locus ID: 117592
• Cytogenetics: 4 E2
• Gene Summary: Beta-1,3-galactosyltransferase that transfers galactose from UDP-galactose to substrates with a terminal beta-linked galactose residue. Has a preference for galactose-beta-1,4-xylose that is found in the linker region of glycosaminoglycans, such as heparan sulfate and chondroitin sulfate. Has no activity towards substrates with terminal glucosamine or galactosamine residues (By similarity).[UniProtKB/Swiss-Prot Function]