Camsap1 (NM_001276360) Mouse Untagged Clone

CAT#: MC229635

Camsap1 (untagged) - Mouse calmodulin regulated spectrin-associated protein 1 (Camsap1), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Camsap1
• Synonyms: 9530003A05Rik; C77823; PRO2405
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229635 representing NM_001276360
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGACGCGGGCGGTCGCTGTGCCGCCGAAGGCTGGAGGAGGATGGAGGCGCCCCCGGAGGGCGCCG
  ACCTGGTGCCGCTGGACCGCTACGACGCGGCGCGGGCCAAGATCGCCGCCAACCTGCAGTGGATCTGTGC
  GAAGGCCTATGGCCTAGACAACATCCCTGAGGACCTCCGAGACCCGTTTTACATCGACCAGTATGAGCAG
  GAGCACATTAAGCCACCCGTTATCAAGCTTCTCCTGTCCAGTGAGCTGTATTGCCGTGTCTGCAGCCTCA
  TCCTAAAAGGGGACCAGGTGGCTACCTTGCAAGGACACCAGTCTGTCATCCAGGCCCTGTCCCGGAAGGG
  CATCTATGTGATGGAGAGTGATGATACCCCTGTGACAGATGCTGACCTCAGCCAGGCACCTATTAAGATG
  AGTGGCCACATGGCGATGGTGGATGCCCTGATGATGGCCTACACTGTGGAGATGATCAGCATTGAGAAGG
  TGGTGGCCAGTGTCAAGCGCTTTTCTACATTCAGCGCCTCAAAAGAACTTCCTTATGACCTCGAGGATGC
  CATGGTATTCTGGATCAATAAGGTAAATCTTAAAATGAGAGAGATAACAGAGAAAGAAGTTAAATTAAAA
  CAGCAGCCACTGGAAAGTCCCGCTCATCAAAAGTCTCCCTCCAAGTGGTATTGGAAGTTAGTACCCGTCC
  GTTATCGGCGAGAGCACCTCTCTGCTAGGCAGTCACCCTACTTCCCGTTGTTGGAGGACTTGATGAGAGA
  CGGCAGTGATGGCGCTGCTCTCTTAGCAGTGGTTCACTATTATTGCCCAGAGCAGATGAAACTGGATGAT
  ATCTGTCTGAAGGAGGTGCCATCAATGGCTGACAGCCTCTATAACATCCGCCTTCTGAGGGAGTTTTCCA
  ACGAGCATCTGAACAAGTGTTTCTATCTCACGCTGGAGGATATGCTGTATGCCCCCCTAGTGCTGAAGCC
  GAACGTTATGGTTTTTATCGCTGAGCTCTTCTGGTGGTTTGAGAACGTCAAACCAGATTTTGTTCAGCCC
  CGAGATATTCAAGAGCTGAAGGATGCTAAAACAGTGTTACAGCAGAAGAGCAGCCGGCCTCCTGTCCCTA
  TCTCCAATGCAACCAAACGCAGCTTCCTGGGTAGCCCTGCTGCCATGAGCCCCGCTGACCAGCCACCATC
  CACCCAGCCTCTTGCTGAAGGCAGTCATCGGTACCACCTCCACTCGGAAGAACCTGAGTGTCTTGGGAAA
  GGAGCTTCCACATTTAGTCCGTCCCATCCTTTGTTGCCACTGAGACAGAAACAGCAAAAAGTGTCACAGA
  CAGAGGAAATCCCTGATCAGCGGCACAGGTCGAATTCTTTAACTCGGGTTGATGGTCAGCCACGAGGTGC
  AATAGGAGCATGGCCAGATAAAAAAAACAGGCCTGTGTCACAGCCAACATCATTTGCTCTCCATCATGCT
  GCAAGTTGTGATGTGGATCCCAGTTCTGGTGACAGTGTCAGCTTAGCTCGCTCTATCAGCAAAGATAGCT
  TGGCATCTAACATTATCCACCTGACCCCACAGAACCAGCCCCATCCCTCCGCTGGAAAGAGCAATGGGAA
  AAGCCTCCTGAGCAATGTCAACATTGAGGATGAGGACGAGGAGCTTGTAGCCATCATCAGGACAGATGTG
  TCTCCTCCTTCCCCACAGATGCCCAGGACCTCACCTCAGGCCCCAGGCCTGGTAGCAAGTATCAGGTCTC
  CCCAGAGACAGGCAGACACTTTGGAGAGTAAGCCTGACAGTTTTTACTTAGAACCCCTGATGCCAGCAGT
  ACTCCGGCCAGCCAAAGAGAAGCAGATTACCACTAAGGAAGATGAGCGTGGAGAAGGAAGGCCAAGGACC
  ATCATGGCCAAGAGGCCTAGTGAGGGCTCCCAGCCTATGGTACGAAAGAAAGTAAGTGGTGGCCATGGCA
  GTCGTGACCTGAACAGAACTTTCACCCCAATTCCTTGTTCAGAATTTGCTGCGAGCATTGATCTTGCGGA
  GGTAGGACCACAGTCAGCAGAAGCTACAGGGGAAGGACAGCCTCTGGCCCTGGGTAGATTTGATACATTA
  CCTCAGGGGCAGGCTGCTGATGGTTTCTTTCTCCATGTAGGCAGGGCTGAGGAAGATGAGGGGAGGTGGT
  ACGTTGGTTCACAAAGTCCTAGCTCCCACGACTCAGAACCCTGGACCATTTTGAGGCAAGACTCGGACTC
  TGATGTAGTAGACGTAGAGGATACAGAGCAGGATTTTATTGGTGAGGACCACCCTGTGGTTATCCCCAGA
  TATGCTGGTGAGGAAGAGTCAGCCAAACTACAGGAGGATATGAAAGTGAAGGAACATGAAGACAAGGATG
  ATGCCAGCGGCCGCTCAAGCCCGTGTCTGAGCACCACTTCTCAGCTCAGCAGCATGTCCATGGCCAGTGG
  AAGTGTAAAGATGACCAGCTTTGCTGAAAGGAAGCTCCAGCGGCTCAACAGCTGTGAGACCAAGTCCAGC
  ACCAGCAGCTCCCAGAAGACCACCCCTGATGCATCTGAAAGCTGCCCAGCCCCTCTGACAACGTGGCGCC
  AGAAGAGGGAGCAGAGTCCAGGCAGGCACAGCAAGGACCCTGCCAGCCTGCTGGCTTCTGAGCTGGTGCA
  GCTGCACATGCAGCTGGAGGAAAAGCGCAGAGCTATTGAAGCCCAGAAGAAAAAGATGGAGGCGCTGTCC
  GCACGGCAGCGTCTGAAGCTGGGCAAGGCAGCCTTTCTGCATGTGGTAAAGAAAGGGAAGGCTGATGGTG
  CCCCACAGCCACTGAGACCCGAACACTTCACAAAGGAATTCACACAGCACAATGGGGAGGACTTGGATGA
  TGGCACTTGTAAAACTGAAGGGTTCCTTGTCAAAGAAGAGCAGAGAGATCTCAGTGACGCTCAGGATGTG
  GCATTTGTCCAGCTACATAAGCCCAGGGACCCAGCTGCTCTGCATGATGGAGAGAAGCACAGAATGATTT
  CTACGGCCCTCCTCGAGGATAGTGTTGGTGAGGTAGATGTGAACGAGTGTGACCTGTCCATTGAGAAGCT
  CAATGAGACCATCAGTACATTGCAGCAGGCTATACTGAAGATCTCCCAGCAGCAAGAGCAGCTTCTCATG
  AAGTCCCCCACAGTGCCAACACCGGGCACTAAAAATAACTGCCAGGACCAAAAAATAAAGGCCCCAGTCC
  ACTTTGTTGAGCCACTCTCTCCAACTGGCGTGCCTGGCCACCGCAAACCACCACGGCTTGGCCAGGGCCG
  GAATTCCCGCTCAGGAAGGCCGGCTGAACTGAAGGTTCCAAAAGACAGGCAGCAGGGTTGTTCTCGGAGC
  AAAACCCCAACACCCAGTGTGGAGACACTCCCACAGTCAAGGTCTTTACCTCCAAGTACCCATCCACGGT
  CACCCTCCGACCCAGGCGGGGAACTCCCTGAGAAGTGTCTCTTCGACAGTTATAGGCTCCATGATGAGAG
  TAACCATCGGACATTTGTTCTGTCCTCTTGCAAAGATGCAAACATTGTATCAGAACAGGTGAACTTTAAA
  GAGGGTCTAGATACAAGTGTGAAAGAGGCAGGGCTGAGCTCCTCTACCATCACAGGCAAAGAACATACTC
  CAGTGGAAGAGCCGCTGAGGAGCAAGGCCAGCCTCATTGAGGTAGACCTCTCTGACCTGAAGGCCCCTGA
  TGAGGATGGAGAGGTGGTTGGCCATGAGAGCTCGGTGGAGCTTGGTGGAGATAGTGACCAGAAGCCTGGG
  GTTGGTTTCTTCTTCAAGGATGAACAGAAAGCAGAAGATGAGCTTGCTAAGAAGCGGGCAGCCTTCCTTT
  TGAAACAGCAGCGCAAGGCTGAAGAGGCTCGTGCGCGCAAGCAGCAGCTGGAGGCAGAAGTGGAGCTCAA
  GCGAGATGAAGCCCGGCGTAAGGCTGAAGAGGATCGACTACGGAAGGAGGAGGAGAAGGCAAGGCGAGAG
  CTCATTAAACAGGAATACTTACGGAGGAAGCAGCAGCAGGCCTTGGAGGAGCAAGGACTTGGCAAACCTA
  AATCAAAGCCTAAAAAGCCTCGGCCAAAGTCAGTTCACCGGGAAGAGTCTTACAGCGACTCTGGCACCAA
  GTGCTCTTCTACCCATAACTTGAGCCAAACTCACTCTGGCTCCAGCCTATCCTTGGCATCTGCAGCAACA
  ACAGAACCTGAGAGTGTTTATTCGGGGGGCACACCTTCTCACCGAGTTGAATCACTGGAAGCTTTACCTA
  TCCTGAGCCGCAACCCCAGTCGGAGCACAGACCGAGACTGGGAGACTGCATCAGCAGCTTCCTCTTTGGC
  CTCTGTGGCTGAGTACACAGGTCCTAAACTCTTTAAGGAGCCCAGTAGCAAATCAAACAAACCAATTATT
  CACAATGCCATCTCCCACTGCTGTCTGGCTGGAAAAGTGAATGAGCCTCACAAGAATTCAATATTGGAGG
  AGCTGGAGAAGTGTGATGCCAACCACTATATTATTCTGTTCCGAGATGCGGGCTGCCAGTTCAGGGCACT
  TTACTGCTACCAACCCGACACTGAAGAAATCTACAAACTCACTGGCACGGGGCCAAAAAGCATCACCAAG
  AAGATGATTGACAAACTATATAAATACAGCTCAGACCGAAAGCAGTTTAACCTGATCCCAGCCAAGACCA
  TGTCAGTCAGCGTGGATGCACTCACGATCCATAACCACTTGTGGCAGCCCAAGCGGCCCACGGTGCCAAA
  GAAGACCCAGACTCGTAAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001276360
• Insert Size: 4782 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001276360.1, NP_001263289.1
• RefSeq Size: 8029 bp
• RefSeq ORF: 4782 bp
• Locus ID: 227634
• UniProt ID: A2AHC3
• Cytogenetics: 2 A3
• Gene Summary: Key microtubule-organizing protein that specifically binds the minus-end of non-centrosomal microtubules and regulates their dynamics and organization. Specifically recognizes growing microtubule minus-ends and stabilizes microtubules. Acts on free microtubule minus-ends that are not capped by microtubule-nucleating proteins or other factors and protects microtubule minus-ends from depolymerization. In contrast to CAMSAP2 and CAMSAP3, tracks along the growing tips of minus-end microtubules without significantly affecting the polymerization rate: binds at the very tip of the microtubules minus-end and acts as a minus-end tracking protein (-TIP) that dissociates from microtubules after allowing tubulin incorporation. Through interaction with spectrin may regulate neurite outgrowth.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) contains an alternate in-frame exon in the 5' coding region compared to variant 1. The resulting protein (isoform 2) is longer compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.