Nrxn3 (NM_001198587) Mouse Untagged Clone

CAT#: MC229632

Nrxn3 (untagged) - Mouse neurexin III (Nrxn3), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nrxn3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229632 representing NM_001198587
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTTTACCCTCCACTCAGTTTTCTTCACCTTGAAGGTGAGCATCTTTCTGGGCTCCCTGGTGGGGC
  TTTGCCTGGGTCTGGAGTTCATGGGCCTTCCTAACCAGTGGGCCCGCTACCTGCGTTGGGATGCAAGCAC
  GCGCAGTGACCTGAGCTTCCAGTTCAAGACCAATGTTTCCACTGGGCTGCTCCTGTATTTGGATGATGGT
  GGTGTCTGTGACTTCCTCTGTCTCTCCCTGGTGGATGGCCGCGTTCAGCTCCGCTTTAGCATGGACTGTG
  CCGAGACCACTGTGCTATCCAACAAGCAGGTGAACGACAGCAGTTGGCACTTCCTCATGGTGAGCCGTGA
  TCGTGTGCGCACTGGGCTGGTGATTGATGGTGAGGGCCAGTCTGGAGAGCTACGGCCCCAGCGGCCTTAT
  ATGGATGTGGTCAGTGATTTGTTCCTCGGTGGCGTCCCCGCTGACATTCGACCTTCTGCCCTGACCCTCG
  ATGGAGTACAGAGCATGCCTGGCTTTAAGGGATTAATGCTGGATCTCAAGTATGGCAACTCGGAACCTCG
  GCTTCTGGGGAGCCAGAGCGTCCAGTTAGAAGCAGAGGGACCCTGTGGCGAGCGTCCCTGTGAAAATGGT
  GGGATCTGCTTCCTCTTGGATGGTCATCCCACCTGTGACTGTTCTACCACTGGCTATGGTGGCACACTCT
  GCTCAGAAGATGTCAGTCAAGGTCCAGGCCTCTCCCATCTTATGATGAGTGAACAAGCACGAGAGGAGAA
  CGTGGCCACCTTCCGAGGCTCAGAGTATCTGTGCTATGACCTGTCCCAGAACCCCATCCAGAGCAGCAGC
  GACGAAATCACCCTTTCCTTTAAGACCTGGCAACGCAATGGGCTCATCCTCCACACTGGCAAGTCGGCCG
  ACTATGTTAACCTGGCTCTGAAGGATGGTGCGGTCTCCTTGGTCATTAACCTGGGGTCCGGGGCCTTTGA
  GGCCATTGTGGAGCCAGTGAATGGGAAATTCAACGACAACGCCTGGCATGATGTCAAAGTGACACGCAAT
  CTTCGGCAGGTGACAATCTCTGTGGATGGCATCCTAACCACAACGGGCTACACTCAAGAGGACTACACCA
  TGCTGGGCTCAGATGACTTCTTCTATGTTGGTGGAAGCCCAAGTACCGCAGACTTGCCAGGTTCACCTGT
  GAGCAACAACTTCATGGGCTGCCTTAAAGAGGTTGTTTATAAGAATAATGACATCCGTCTAGAGCTGTCT
  CGCTTGGCCCGGATTGGAGACACCAAGATGAAAATCTATGGTGAAGTTGTGTTCAAGTGTGAGAACGTGG
  CCACACTGGATCCCATCAACTTTGAGACCCCAGAGGCTTACATCAGCTTGCCCAAGTGGAACACCAAACG
  TATGGGCTCCATTTCCTTTGACTTTCGCACCACTGAGCCCAACGGCCTGATTCTCTTCACTCACGGGAAG
  CCTCAAGAAAGGAAAGATGTCCGGAGCCAAAAGAACACAAAAGTTGACTTCTTTGCTGTGGAACTTCTTG
  ATGGCAACCTGTATTTGCTGCTTGACATGGGCTCTGGCACCATCAAAGTGAAGGCCACTCAGAAGAAGGC
  CAATGATGGGGAATGGTACCACGTGGATATTCAGCGTGATGGCAGATCAGGTACCATATCTGTGAACAGC
  AGGCGCACGCCATTCACCGCCAGTGGGGAGAGTGAAATTCTCGACCTGGAGGGGGACATGTATCTGGGCG
  GGCTGCCAGAGAACCGAGCTGGCCTCATCCTCCCCACGGAGCTCTGGACCGCCATGCTCAACTACGGTTA
  CGTGGGCTGCATCCGCGACCTGTTCATCGATGGGCGCAGCAAGAACATCCGGCAGCTGGCGGAGATGCAG
  AACGCAGCAGGCGTCAAGTCCTCCTGTTCACGCATGAGCGCCAAGCAGTGTGACAGCTACCCCTGCAAGA
  ACAACGCGGTGTGCAAGGACGGCTGGAACCGCTTCATCTGCGACTGCACTGGCACCGGCTACTGGGGGAG
  AACCTGCGAAAGAGAGGCATCTATCCTGAGCTATGACGGCAGCATGTACATGAAGGTCATCATGCCCATG
  GTGATGCACACGGAGGCAGAGGATGTGTCCTTCCGCTTCATGTCACAGCGAGCCTATGGGCTACTGGTAG
  CCACAACCTCCAGGGATTCTGCAGATACACTGCGTCTAGAGTTGGATGGTGGGCGTGTCAAGCTCATGGT
  TAACTTAGACTGTATCAGGATAAACTGTAACTCCAGCAAAGGACCTGAGACCCTGTATGCAGGGCAGAAG
  CTCAATGACAATGAGTGGCACACCGTCCGGGTAGTGCGGAGAGGAAAAAGCCTTAAGCTAACAGTGGATG
  ATGATGTGGCTGAGGGGACAATGGTGGGCGACCACACCCGCCTGGAATTCCACAATATTGAAACTGGTAT
  CATGACTGAGAAGCGTTACATCTCTGTGGTCCCCTCCAGTTTCATTGGCCATCTACAGAGCCTCATGTTC
  AATGGCTTACTGTACATTGATTTGTGCAAAAATGGCGACATCGACTACTGTGAACTGAAGGCTCGCTTTG
  GACTGAGAAACATCATCGCCGACCCTGTCACTTTTAAGACCAAGAGCAGCTACCTGACCCTCGCCACCCT
  ACAGGCTTACACCTCCATGCACCTCTTCTTCCAGTTCAAGACCACTTCAGCTGATGGCTTCATTCTCTTC
  AATAGCGGAGATGGCAATGATTTTATTGCAGTTGAGCTGGTCAAGGGGTACATACACTATGTGTTTGATC
  TCGGCAATGGTCCCAATGTGATCAAAGGCAACAGTGACCGCCCCCTGAATGACAACCAGTGGCACAACGT
  GGTCATCACCAGGGACAGCAGTAACACCCACAGTCTGAAGGTGGACACCAAGGTAGTCACTCAGGTCATC
  AATGGTGCCAAAAATCTGGATTTGAAAGGTGACCTCTATATGGCTGGCTTAGCCCAGGGCATGTACAGCA
  ACCTTCCCAAGCTTGTGGCCTCCAGGGATGGATTTCAGGGCTGCCTGGCCTCTGTGGACTTGAATGGACG
  CCTGCCTGATCTCATCAACGATGCTCTCCACAGGAGTGGACAGATCGAGCGAGGCTGTGAAGGACCCAGT
  ACAACCTGTCAGGAGGATTCATGCGCCAACCAGGGAGTTTGCATGCAGCAGTGGGAAGGTTTCACCTGTG
  ACTGCTCCATGACATCATATTCTGGAAACCAGTGCAATGACCCTGGTGCAACATACATCTTTGGGAAAAG
  CGGTGGCCTCATCCTCTATACCTGGCCAGCAAATGACAGACCCAGCACACGCTCTGACCGTCTCGCCGTG
  GGCTTCAGCACTACTGTGAAGGATGGTATCTTAGTACGCATTGACAGCGCCCCTGGACTTGGCGACTTCC
  TCCAGCTTCACATAGAACAAGGGAAAATTGGAGTTGTCTTCAATATTGGCACAGTTGACATCTCCATCAA
  AGAAGAGAGAACTCCTGTCAATGATGGCAAATACCACGTTGTGCGCTTCACCAGGAATGGGGGAAATGCT
  ACACTTCAGGTGGACAACTGGCCAGTGAATGAGCACTATCCTACAGGCAACACTGATAATGAACGCCTCC
  AAATGGTAAAACAGAAAATCCCCTTCAAATATAACCGGCCCGTAGAGGAGTGGCTGCAGGAAAAAGGCCG
  GCAGCTAACCATCTTCAACACCCAGGCGCAAATAGCCATCGGAGGAAAGGACAAAGGACGTCTCTTCCAA
  GGTCAACTCTCTGGGCTCTATTATGATGGTTTGAAAGTACTGAACATGGCAGCTGAGAACAACCCTAATA
  TTAAAATCAATGGAAGTGTCCGGCTAGTGGGAGAAGTCCCATCAGTCTCAGGAACAACACAGACAACGTC
  CATGCCACCTGAAATGTCTACCACCGTCATGGAAACCACCACCACCATGGCTACGACCACCACCCGAAAG
  AACCGCTCTACAGCCAGCATTCAGCCCACGTCAGATGATCTTGTTTCATCTGCTGAATGTTCAAGTGATG
  ATGAAGACTTTGTCGAATGTGAACCAAGTACAGATAAGAGTCTTTCCACTTCAATCTTCGAAGGTGGCTA
  CAAAGCACATGCGCCCAAGTGGGAATCCAAGGACTTTAGACCTAACAAAGTCTCGGAAACTAGTAGAACT
  ACAACCACCTCTTTGTCCCCTGAGCTGATCCGCTTCACAGCGTCCTCCTCGTCTGGGATGGTGCCCAAAT
  TGCCAGCTGGCAAAATGAATAACCGTGATCTCAAACCCCAGCCTGATATAGTCTTGCTTCCGTTGCCCAC
  TGCCTATGAGCTAGACAGCACCAAACTGAAGAGCCCACTAATTACTTCCCCCATGTTCCGTAATGTGCCC
  ACAGCAAACCCCACGGAGCCAGGAATCAGACGGGTTCCGGGGGCCTCAGAGGTGATCCGGGAGTCCAGCA
  GTACAACAGGGATGGTCGTCGGCATTGTGGCTGCTGCCGCCCTCTGCATCTTGATCCTCCTGTACGCCAT
  GTACAAGTACAGGAACAGGGACGAGGGGTCCTATCAAGTGGACGAGACGAGGAACTACATCAGCAACTCG
  GCCCAGAGCAACGGCACGCTCATGAAGGAGAAGCAAGCCAGCTCCAAGAGCGGCCATAAGAAACAGAAAA
  ACAAGGACAAGGAGTATTATGTGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001198587
• Insert Size: 4716 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001198587.3, NP_001185516.2
• RefSeq Size: 7933 bp
• RefSeq ORF: 4716 bp
• Locus ID: 18191
• UniProt ID: Q6P9K9
• Cytogenetics: 12 42.94 cM
• Gene Summary: This gene encodes a member of a family of proteins that function in the nervous system as receptors and cell adhesion molecules. Extensive alternative splicing and the use of alternative promoters results in multiple transcript variants for this gene, but the full-length nature of many of these variants has not been determined. Transcripts that initiate from an upstream promoter encode alpha isoforms, which contain epidermal growth factor-like (EGF-like) sequences and laminin G domains. Transcripts initiating from the downstream promoter encode beta isoforms, which lack EGF-like sequences. [provided by RefSeq, Dec 2012]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). This variant encodes an alpha isoform of neurexin 3.