Cobl (NM_001282994) Mouse Untagged Clone

CAT#: MC229562

Cobl (untagged) - Mouse cordon-bleu WH2 repeat (Cobl), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cobl
• Synonyms: 4732460E13; C530045F18Rik; mKIAA0633
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229562 representing NM_001282994
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTGTGACGACAAAGAAAGGAAGATGAAGGCCCGTGCTCCGCCACCTCCTGGAAAGCCTGCTGCCC
  AGAATGTCCACAGCGAACAGAAACTGCCCCATGATGCCACCCTGGGATCCCAGCAAAGCCTGGTCTACAT
  GAAGGAGGCACTGCAGAACAGCACCTTGGACATCACCGTGGTTCTGCCCAGTGGACTGGAGAAGCAGAGT
  GTGGTTAGTGGGAGCCACGCAATGATGGACCTACTGGTTGAACTCTGCCTTCAGAACCACCTGAATCCTT
  CTCACCACGTCCTAGAGATCTGGTCTTCTGAGACCCAACAGCCTTTGAGTTTTAAGCCAAATACGTTGAT
  TGGGTCCTTGAATGTGCATACTGTACTTCTGAAAGAAAAAGTCCCTGAAGAAAGAGTTAAACCTGGCCTA
  ACCAAGGCTCCTGAGAAATCTGTACGACTGGTAGTGAACTACCTGAGAACGCAGAAGGCTGTCGTGCGGG
  TGAGCCCTGAGGTGCCACTACAGAATATTCTGCCAGTCATCTGTGCGAAGTGTGAGGTCAACCCAGAACA
  CGTGATTCTGCTCAGAGACAATGTTGCCGGGGAGGAGCTGGAGCTGTCCAAGTCCCTGAATGAACTAGGG
  ATCAAGGAGCTGTATGCGTGGGACAACAGAAGAGAAATGTTTAGAAAATCATCACTTGGCAATGATGAGA
  CAGACAAAGAGAAGAAAAAGTTTCTGGGATTTTTCAAAGCTAATAAAAGGAGCAACAGTAAGGCTGAGCA
  TCTTGGGCTGTCAGGTGCAGATAGTGATGAGGACCCGGCGAAGTCAGCATCTGGAGGGGACTTGAACGGC
  TGCGTAACAACACCCAACTCACCATCCTTGCATTCTCGGTCCCTAACACTGGGTCCCTCCCTCTCCCTGG
  GCAACATCTCTGGAGTGTCTATGAAGTCAGACATGAAGAAGCGCAGAGCCCCTCCTCCTCCAAGTCCCAA
  GCTGCTGGGTCAAGACAAGGTATCAGAAAAGGCCTCCCTCAGCTCACAGGCTGATCTCCAGAAGAAGAAG
  AGGCGGGCGCCAGCTCCTCCTCCACCACAGCAGCCACCACCAAGTCCTGTGGTCCCCAACCGCAAGGAAG
  ATAAGGAAGAGAACAGGAAGAGCACAGTGGGTGTTGGACGTCAGGTGCCACAAAAGCCTCCCAGAGGCAC
  AGCTCGGGGCCCACCCCAGTTAGTGCTCCCCCCGCCCCCACCCTACCCTCCTCCTGACACAGATGTGACA
  GAGCCTGTCACCTTTCCTGGGGAAGGCGCTGGTTCCGAGACCTCAGAGCTGAGACCCAAACTGAGTCTGC
  CTCTGGGGCCTGGCAGCCACTGCAGCATGGGTGGAGTCTCTCAGGTGCCAGCGGAGAGTGAGGAGACAGC
  ATCTGAGGACACAACCGAGGACTCGGGGGTCATGAGCTCTCCCTCAGATGCCATCTCCCTGGACTCACAA
  CAAGACAGCATGAGATCCAAGGACAAATGGTCCACAGACCAGGAAGACGGCAGTGACCAGGACCTGGCTG
  GAACTCCAGAACTGGGTCCCCAAAAGAGCCCATCATGGGGGAAAAGTGGTTCAGGAAGCTCAATTCTAAG
  GACTGAGAAAGCCACCATGCCTACCAATGATGATGAAGACCTATTCATCACTGGCCACTTACATCAGACC
  CTGGCAGAACTTGATGAAGACCTGGAAGGAATGGAAGAAAATTATGAAACAGATACTAGCTCTCTAACCA
  ACTCTGTCAATGGTGTATCCAACCACAGTCTGCAAGAAGCCATAATCCCTGATAGTGGCGTGGATGACAT
  CCCAGTCACTTTTATCGGGGAAGTTTCTGATGAGCCTTTTGACTCAGGGCTGTTCTCCAGTAGATGTAAC
  AATGCCACTACTTTTAACACGGGGAGCATTGCTAGCCAAAGATCTCATCTGTCCCCATCTCAGACTGAGC
  ATAGCCAGCCATTCGTAAGGACAAGTAGAAAAGAGCCTGATCCCTCCCCTCCTTCCCAAGATAACAGGAA
  GAGAAATCAGCCAACCTTAGCCAACACATCTGAAAATGAGAATCCAGTTGAAACAGACCCCACAGTTACA
  TCACTTGTTTCAAAGCTTTTAATAGATGACCCAAAAGCAAAGGATAAAGGCAAAGTGCATGGTTCTAGTC
  ACAGTGAGAAGACACAGGCAGGTCATGGAATAAACTCACTGCGAGTGAATCCAAGAGATGGTAAGGATGA
  GAGCTCCAATTCAGCACCCCCACCATGGTCTCATCATGGCCAGGCCTTAGGGGGAAGCTATGGACTCAAG
  TATGGCCTCACGACATATAAAATTGTTCCTCCCAAATCAGAGATGAGGTGTTATGACAGGGATGTGTCCC
  TCTCCACTGGTGCCATTAAGATTGATGAACTAGGGAATCTGGTGAGTCCTCACATGAACGGAAGCAGGAC
  CATATCCCCACCATCAGCTGTTGTCGAAACAGACACTCCACCTATTGGAAAAGTCAAAGAGTTCTGGAGG
  CGCAACTCAATGGAAAAGTACCTTAATGGGCCGGCTGAGTGCACTATCAAAAGGGCCCCCTCCACCACTA
  TCACTGCCACACCAGAAAAACCACAGCAAGATAATGGGATGAAGGCAGCCTTCACAGTGACCACTCCCCA
  GCAGCAGCCTGCCTCACAGGAATATGGGGCACACCTGGAGGAAGAGAGAAGCCGGCCACAGTCAGCAGTC
  TCTTGTTCTGTGAAAGTGCCAGCATCTAATCCCACAGACATCACATTCCTCAAGCCTCAAAGAAGAACAT
  CCAGCCAGTATGTGGCCTCTGCTATTGCCAAGAAGATGGGGCCTCCAAAAGTCCATGCTGATGTGGTGAG
  ACCACATAAGGCAACCACTGAACAGTGTCATGAAGAAGCAAAGCTGGCTAGATCTCCTCCCACCAGGAAA
  GATGATGCAGCTCCCAACCTACACTCAGAGGCAAGGCAGCATGAACATGGGACAAACCAGAGCTCTGTCT
  GCCTTCCCAGCAACCCTGGTGTGCAATTGCCAGCAGGAGGCCATCCCAAAGTAGAGGTCAACAGCACATA
  TGGGAAGTCATCGACTCAAGACTATCCTGCTGCTGTCCACAGAAACTCCTATTTCCTCCCAGGCAGATCT
  TCCCATAGGGATCGTGTTTCTGTGGGACAGAGCTGTGGTTTCAATGAAAAGCAAACTACAAGTAACCAAA
  AGGCAAATTCAACATCTAACTTCTCGCAGGCACTGGACAAGGCGCATCCACCCCCTCTGCTCTTGGCAGA
  GGCCCGTGACTCTGGGAGGATACTGATGAATGGCTCTGCACGAACCCCAGGAAATTGTGAGCCCCCCCAC
  TCTCCAAAAGAATCCACCCTGACTAGCTATATTATCTTACAGACAGAGGAAAAGCCCAGTTCGTTATCTA
  CAGATGGTCAAGACGCAGATGATACTCTACCGTCCAGCATTTTTGGGCCAAAGAAAAAGTTCAAGCCTGT
  CATTCAAAGGCCACTCCCAAAAGATGTATCCCTGCACAGTGCCCTGATGGAAGCTATCCACTCATCAGGA
  GGGAGAGAAAAGCTCCGGAAGACTGCAGAACAGACGTCAGAAGGAAGGCCAAAGAAACCATCCTACGTGG
  AGGCAGAGAGTGAGCGATCTGCCCTTCTGGCAGCCATTCGGGGTCACAGTGGGACTCTCAGCCTCAGGAA
  GGTGTCATCCCTTGCCTCTGAGGAGCTCCAGAGCTTCCGCAATGCTGCACTCGGGGCTCCAGGTTTGGAT
  AAACCTCAGCAAGAAGACCTTGGCCTTCCACCCCCACCTGCCCTGCCACCACCACCCGCTCCAGCCCCCC
  AGGCTCCTTCAGCCTCCGTAACAGTTTCTAGGTTCAGCACCGGCACCCCCAGCAACTCAGTGAATGCTAG
  ACAAGCCTTGATGGATGCCATCCGCTCGGGCACAGGAGCTGCAAGACTAAGAAAGGTTCCCTTGCTCGTG
  TGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001282994
• Insert Size: 3993 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282994.1, NP_001269923.1
• RefSeq Size: 5464 bp
• RefSeq ORF: 3993 bp
• Locus ID: 12808
• Cytogenetics: 11 7.3 cM
• Gene Summary: Plays an important role in the reorganization of the actin cytoskeleton. Binds to and sequesters actin monomers (G actin). Nucleates actin polymerization by assembling three actin monomers in cross-filament orientation and thereby promotes growth of actin filaments at the barbed end. Can also mediate actin depolymerization at barbed ends and severing of actin filaments. Promotes formation of cell ruffles. Regulates neuron morphogenesis and increases branching of axons and dendrites. Regulates dendrite branching in Purkinje cells.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (3) has a shorter and distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.