4932416K20Rik (NM_001002775) Mouse Untagged Clone

CAT#: MC229318

4932416K20Rik (untagged) - Mouse RIKEN cDNA 4932416K20 gene (4932416K20Rik)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: 4932416K20Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229318 representing NM_001002775
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCGCAGCAACTGCCCTAAAGGAGAAAGTTTTCCTTGCAGCTTAACTAGCTGCCAACCCTACATCC
  AGCTGGATCGGGATTATCACTCTACACATCCAGTGGCCCTGGAGAAGCTGCCCCTGCCTGGCCAGCATCA
  TCCGGTTGCTACAGCCAAGGTGAATCTGCCTGTGCAGCGAATCCACCTTCAGTGTTTCCCCTGCCCCACT
  GGTCTCTACGCCATCCCCAACAGGCAGAACAACTTCAAGATGTTCTGTCCCTCCCGAACCATGAAAATCC
  CTCCTCTAGGGACAAATCCTTCTTTTCCCACTTCCTGTCCAGCCTGGGTGGTTCAGGCTGAAGAAGTTCT
  GACATTGGACCACCGGTCTCTTCCTTTCTCAAAGGAAACTGAGGCAAAAGTCAAAGAACTTAAAAACAGG
  AGGAGTGAAGAGAAGGGGAAGGGGAAAAAAGAGGAGGGAGAGGAAGAGGAGGAAGAGAAGGAAGTAAAAC
  AGCAGCAGGAGGAACAGGTGGAGGATGAAAAACTGGAGGAGGAGGAAGAAGATGAAGAGGAGGAGGGAGA
  GGAGGAGGAAGAGGAGGAAGAGAAGGGAGAAGATGAAGAAGAGGAGGAGGGAGAGGAAGAAGAGGGAGAG
  GATGAAGAGGAGGAGGGAGAGGAGGAAGAGGAAGAGGAGGAAGAGGAAGAAGAGGAAGAGGAGGAAGAGG
  AGGAAGAGGAGGAGGGAGAGGAAGAAGAGGGAGAAGATGAAGAGGAGGGAGAGGAGGAGGAAGAGGAGGA
  AGAGGAGGAGGGAGAGGAAAAAGAGGGAGAGGATGAAGAGGAGGGAGAGGAGGAGGAAGAGGAGGTAGAA
  GATGAAGAGGAGGTGGGAGAGGAGGAGGAAGAGGAGGAAGAGGAGGAGGGAGAGGAAGAAGAGGAGGAGG
  GAGAGGAAGAAGAGGAAGGAGAAGATAAAGAGGTGGAGGGAGAGGAGGAGGAAGAGAAGGAAGAGGAGGA
  GGGAGAGGAGGAGGAAGAGGAGGAAGAGGAGGAAGATAACAGCTGGCTCCTGGAGGAGACAGAAATTCAA
  GATGTTAAGGAAAGGTGTTCATCCACTAGCCTCATGCAAGCAGCCACATGTGTGAGCTTAGCGGCCAAAT
  CTACCATAATATCTGAAAATCTACCCTGTGGACCTCAAGAAGGATATCAGCATCCCCATGATGTAAAAAC
  TGTGGAGATGACTGCCCTTTTATCTTCCAGGGAGTTCACTGCCAGCTCTTGTAGACCTGCTGGAGTGGTC
  CTCACTCATGTGTGGTCTGGCGAGGATGTGTCGGGGTGCCTGACACCAGCCTCAGCCAGCTGCTCCTCAC
  ATCGGGAGACCTTTCCCAAGAAAGAACAGAGGACAAAAGGTCTTCCAGGCCCTGCACATTCTCAGCCTCA
  GGCTAGTGCATCAGGGAGCCCTGTTACTCCTGTGTCAGCATTAAAGAGACCCTGCAAGAGAAAGGCAGCC
  ACACCAGAGTTGCTGGTGCCACCCTCAGAGGTTCTACAACTACTGCAGGATGAAGGTGACTGGCCCCCTC
  CTCCCAAGATTCCACACCTTGAGGAAAAGAAAGAATCAGACACCATAAAGAACAAGAAGGGTCAGGCCAT
  CCAGATCTCAGATGATAAAGTGAATGTCTTAGAACCTAAAAGCCAGGAGGTGTCAGTCCGCACTGTCCCT
  CAGCCTGATCTGCCAAGGTCCCCACCTTCTTCAGGACCTGGAGTCGCTCTGTCCTTACCCACTGATGGGG
  TTTCCAGCCTTGGTGGTCCTGCCTCCACTCCCTTGTTATGCCAGGCTGTTCGATCTCCAATCCCATCTGT
  CCCTTCACCTTCCCCCACACCAAGTATACCCACAATCCCTATTTCTCCTTTGGTTCTCCCTTCTGACTCT
  TTTCCTCCCTGTCAGGCCAGGCCCTGTGTGGAGACTCCACCAGATTTCAAGAAGAAAGACCCTACTAATG
  TGGCCACAACTCCTGCCATTTCTGACCTCCCCAGGCCTGGGGCTGAAGCTTTGTCTTTCAATCCCTCCTC
  CTGCAGGAGGGACTCCACAGTGTCAGTGTGCACAGCTAGCCACCCTCCTCTCCCCTTCTCTGCGGCTCCT
  CTATCCCTCTTCCCCAGGAACCCTCCCACAGTCTTCCCTACCTCAGCATCTCAGGACTGTGAAGACATGT
  ACTGGGAGCCATCTCCGTGGCCTGCAGAGGTGGACATGGATACCACGCCCCCTTCTGAGGCGGCTATTCT
  GAGCTCACCCACTGTCAGCAGTGGTAGCTCCTTACCATTTGCTCCTAGCCATGGCTGTATCCAGCAGAGG
  ATGGTTGCAAACGCAACCGTGTCCATTAGCCTACCTTCATGCAAAGTCCCCTGCTATACTAGCTTTGGGA
  ACAATCTGTTTACTCCAAGGCCCACCATTGGGCAGATGCACGGAGTGTCTCCCGCTGGTAATCCTGGTGT
  TTGGATCCAACCTGGGCTGGTAGCAGCTCTCCCTCCCATGGCAGCAGTAACCCCATCTCCTGCTGCTGTG
  GACTTTGAATTCATGGATACCACCCCACCTTCTAAAGCTTTCATCTTTCATTCTCCCCCTGCCACTGACA
  ATGTCTCCAACAGTGTGCCTCCCCAGTCAGTTTGGGGGCATACTCCAGCTTGCAAGCCTGCTGGAGCTAC
  CCCTAGGACGACCCCTGCATTGCTAAGTGGGCCGCCAGCTGATGGCTGTGTCTTTATGAGAAATCCCCCC
  ACCTTAAGAGCAGCACCTGGCTTGACGTCACGAGCTGGCCCACCTGCTGGAGGTAGCTCGACTCACCTAG
  GATTTGTAGGCCCAGCATCACCTGCTCACCATGGCAGGCCTGATGGCACTGAGCTAGCCTACAGGAAGAG
  CCCACGTGCTTCGTCCATCGCCACATCGACCTCAGGGTTGACTCTCAAGGCAAGAATGGGTTCAGGGAAC
  TGCAGCTCATCATCCACCAACTTCACTCCCAGCACCCCAACATTGGTGAAACCTGTGGTGCCAGTAGAGG
  GGGAGAACTGTGGGCGTGGCACGCACCTGCAAGCACACCTACCCACAGCTCGCTACTACATTGCTAGGAG
  GAAGGGGAAGGACAATGGAGTTACTGTCGCCCCCATTGTCCTCAAACTTTGCAGCTCATCTGCACACACT
  GGTCATGCTGTGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001002775
• Insert Size: 3165 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001002775.2, NP_001002775.2
• RefSeq Size: 3556 bp
• RefSeq ORF: 3165 bp
• Locus ID: 442809
• Cytogenetics: 8 D3