Wipf1 (NM_001289723) Mouse Untagged Clone

CAT#: MC228007

Wipf1 (untagged) - Mouse WAS/WASL interacting protein family, member 1 (Wipf1), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Wipf1
• Synonyms: AI115543; D2Ertd120e; Waspip; WIP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228007 representing NM_001289723
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGTCCCTCCCCCACCAGCACCCCCACCGCCACCAACATTTGCACTCGCTAACACTGAGAAGCCCA
  CCTTGAACAAGACAGAGCAGGCTGGGAGGAATGCTCTTCTCTCTGACATCAGCAAAGGGAAGAAGCTAAA
  GAAGACGGTCACCAATGACAGAAGTGCCCCAATATTGGACAAACCAAAGGGAGCCGGCGCCAGTGCTGGT
  GGCTATGGTGGAGGCGGCGGAGGTGGTGGTGGAGGAGGAGGAGGAGGAGGAGGCAGCGGTGGAAATTTTG
  GAGGGGGTGGTCCTCCAGGACTGGGAGGACTATTCCAGGCTGGAATGCCGAAGCTGAGGTCCACCGCCAA
  CAGGGATAATGATTCTGGAGGAAGCCGACCACCGATTTTGCCACCGGGAGGAAGAGCCACATCTGCCAAG
  CCCTTTTCACCCCCGAGTGGCCCGGGGAGGTTTCCCGCACCCTCCCCAGGCCACAGGAGTGGGCCCCCAG
  AGCCACCCAGGAACAGAATGCCTCCCCCGAGGCCTGACGTGGGCTCAAAACCAGATAGCCTTCCCCCTCC
  GGTACCAAATACGCCAAGACCTGTTCCGTCGAGTCTGCACAACCGGGGATCCCCAGCAGGGCTGGGAGCC
  CCCAGGCCCCCCTTTCCCGGGAACCGAGGTGCTGCTTTTGGAGCAGGCTCTGCGCGCCAGAACCCCTCCG
  GCTCCTCCTCTCCGTTTCCTAGGCCCCCTTTGCCCCCCACCCCAAGCCGAGCCTTGGATGACAAGCCCCC
  TCCACCACCTCCTCCCGTGGGCAACAGGCCCTCCATGCACAGAGAAGCGGTGCCCCCTCCCCCCTCACAG
  ACCAGCAAACCTCCAGTGCCTTCCACCCCGCGGCCTGGGTTGGGTTCGCAGGCACCACCTCCTCCTCCAC
  CGCCCAGCCGGCCAGGGCCCCCTCCTCTGCCTCCAGCTTCCAACGACGAGATCCCCAGGCTGCCACAGCG
  GAACCTGTCCCTCACGTCCTCTGCACCTCCATTGCCTTCACCTGGACGCTCAGGGCCTCTCCCTCCCCCA
  CCCAGCGAGAGGCCCCCTCCTCCTGTAAGGGATCCACCAGGCAGATCAGGCCCCCTCCCACCACCACCTC
  CAATAAACAGAAATGGAAGCACAGCTCGGGCCCTGCCTGCCACCCCTCAGTTGCCATCCAGGAGTGGAAT
  GGACAGTCCCAGAAGTGGGCCTAGGCCTCCTCTTCCTCCTGACAGGCCTGGTGCTGGGGCGCCGCCCCCA
  CCCCCACCCTCCACATCAGTTCGAAATGGCTTCCAAGACTCATCGTGTGAAGATGAGTGGGAAAGCAGAT
  TCTACTTCCATCCGATTTCTGACTTGCCGCCTCCAGAGCCATACGTACCGACAACCAAGACGTATCCCAG
  CAAACTGGCCAGAAACGAAAGCCGAAGTGGATCCAACAGAAGAGAAAGGGGCGCCCCACCCCTTCCTCCC
  ATCCCGAGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289723
• Insert Size: 1482 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289723.1, NP_001276652.1
• RefSeq Size: 4451 bp
• RefSeq ORF: 1482 bp
• Locus ID: 215280
• UniProt ID: Q8K1I7
• Cytogenetics: 2 43.68 cM
• Gene Summary: Plays a role in the reorganization of the actin cytoskeleton. Contributes with NCK1 and GRB2 in the recruitment and activation of WASL. May participate in regulating the subcellular localization of WASL, resulting in the disassembly of stress fibers in favor of filopodia formation (By similarity). Plays a role in the formation of cell ruffles.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1, 2 and 3 encode the same protein.