Cep57l1 (NM_001243074) Mouse Untagged Clone

CAT#: MC227634

Cep57l1 (untagged) - Mouse centrosomal protein 57-like 1 (Cep57l1), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cep57l1
• Synonyms: 2410017P07Rik; 4930484D11Rik; AI607327; cep57R
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227634 representing NM_001243074
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCACCGCGACACTTGTCTTTGCCCGCGAACCAACGGTTCTGGAGGCCGTGGTTAACTGGGAGCGTCC
  ATCTGACAATGGATTCTGAATTATCACAGAGTATGGTTGGGAGCTATCTTAACCCTCCAGAAAGAATGCA
  TCTTCCATCATTCACGCAAAATGAAGCATTTCAGAATTGCCACCCTGGGACCCCTCCTAAAATGTTTAAT
  AGTCCAAACAACCAAGCACTTGTTTCAGCATTAAAAACTCTCCAGGAAAAGATTCGTCGTCTCGAGCTGG
  AGAGGACACAGGCTGAAGACAACCTGAACCTTCTGTCCAGAGAAGCAGCGCAGTATAAGAAGGCCCTAGA
  GGAGGAGACCAACGAGAGGAACCTCGCACACCAGGAGCTGATTAAACAAAAGAAAGATATAAGCATACAA
  TTAAGCTCAGCCCAGTCTCGTTGTATTCTTCTAGAGAAGCAATTGGAATATACAAAAAGAATGGTTCTCA
  ATGTAGAACGAGAAAAGACTATGATCCTAGAACAACAGGCCCAGCTTCAAAGGGAAAAAGAACAAGATCA
  GATGAAGCTGCACGCTAAACTTGAAAAGCTCCATGTCCTAGAGAAAGAGTGTCTGAGACTGACGGCGACT
  CGGCAGACTGCGGAGGACAAGATTAAATGTCTAGAAGAAAAACTGAAGGAGGAGGAGCATCAGCGCAGGC
  TGTTTCAAGACAGAGCCTGTGAGAAAACTAACTGCTTAAAACGAGAGCCGCCTCAGCAAAGGGACCACAA
  GTTTAGAACACCAACTTTTGAGAGAAAGAAGCCTTTCAGAACAACTTCCCAGGCTAGAGCTAACCCTCAG
  AGCTCAGGAGAGCCCGTTTCCATTTGTGACAGTTTGTCTGAACTCTTGATGACAATGGAAGAGGAGCTGG
  ACCAAATGAATATGGAGCACAGAGAGCTCCTGAGACAGATGATGCAGCCTGGGAACCACTCGGTCTCTGA
  GGACATAGAGCAGGAGCTGGAGCAGTTAGCCAAGAAGATGGAAAGCAAAGGTGACCAGATCTCCAAGCTC
  AAGAAGCATCAAGACAGTGTCCGTAAACTGCAGGAAAAAATTGAGAACTCAAGGATAAATGAATCTTCAG
  GTATCCATGGCAACCCTAAAGGATCTAAGAACTTAAAAACCAGCCCCCGGAAATGTGTGAGTGAAACCAG
  CTCTTTTCAGAGAGACAGAGGCTTTCAACCAGTGCAGGTCCACAGCCTGCAGTCGAAGCTGAGGCGCGAT
  GACATCAAGTGGGAGCAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001243074
• Insert Size: 1281 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001243074.1, NP_001230003.1
• RefSeq Size: 2233 bp
• RefSeq ORF: 1281 bp
• Locus ID: 103268
• UniProt ID: Q8VDS7
• Cytogenetics: 10 B2
• Gene Summary: Centrosomal protein which may be required for microtubule attachment to centrosomes.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR and contains multiple differences in the coding region, compared to variant 1. It initiates translation at an alternate upstream start codon. The encoded isoform (3) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.