Ifi211 (NM_001301745) Mouse Untagged Clone

CAT#: MC227625

Mnda (untagged) - Mouse myeloid cell nuclear differentiation antigen (Mnda), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ifi211
• Synonyms: A730048F03; ifi-205-B; Ifi20; Ifi205b; Mn; Mnda
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227625 representing NM_001301745
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGAATGAATACAAGAGAATTGTTCTGCTGAGAGGACTTGAATGTATCAATAAGCATTATTTTAGCT
  TATTTAAGTCATTGCTGGCCAGAGATTTAAATCTGGAAAGAGACAACCAAGAGCAATACACCACGATTCA
  GATTGCTAACATGATGGAAGAGAAATTTCCAGCTGATTCTGGATTGGGCAAACTGATTGAGTTTTGTGAA
  GAAGTACCAGCTCTTAGAAAACGAGCTGAAATTCTTAAAAAAGAGAGATCAGAAGGAGAAACATCACTGG
  AAAAAAATGGTCAAGAAGCAGGTCCTGCAACACCTACATCAACTACAAGCCACATGTTAGCATCTGAAAG
  AGGCGAGACTTCTGCAACCCAGGAAGAGACTTCCACAGCTCAGGCGGGGACTTCCACAGCTCAGGCGGGG
  ACTTCCACAGCTCAGGCGGGGACTTCCACAGCCCAGAAAAGAAAAAGTATGAGAGAAGAAGAGACTGGAG
  TGAAAAAGAGCAAGGCGGCTAAGGAACCAGATCAGCCTCCCTGTTGTGAAGAACCCACAGCCATGTGCCA
  GTCACCAATACTCCACAGCTCATCTTCGGCTTCATCTAACATTCTTTCGGCTAAGAACCAAAAATCACAA
  CCCCAGAACCAGAACATTCCCAGAGGTGCTGTTCTCCACTCAGAGCCCCTGACAGTGATGGTGCTCACTG
  CAACAGACCCGTTTGAATATGAATCACCAGAACATGAAGTAAAGAACATGTTTCATGCTACAGTGGCTAC
  AGTGAGCCAGTATTTCCATGTGAAAGTTTTCAACATCGATTTGAAAGAGAAGTTCACAAAAAATAATTTT
  ATCACCATATCCAATTACTTTGAGAGCAAAGGCATCCTGGAGATCAATGAGACTTCCTCTGTGTTAGAGG
  CTGCTCCTAAACAAATGATTGAAGTGCCCAACTGTATTACCAGAAATGCAAATGCCAGTCCTAAGATCTG
  TGATATTCAAAAGGGTACTTCTGGAACAGTGTTCTATGGAGTGTTTACATTACACAAGAAAAAAGTGAAA
  ACACAGAACACAAGCTATGAAATAAAAGATGGTTCAGGAAGCATAGAAGTTGTGGGGAGTGGACAATGGC
  ACAACATCAACTGTAAGGAAGGAGATAAGCTCCACCTCTTCTGCTTTCACCTGAAAAGAGAAAGAGGACA
  ACCAAAGTTAGTGTGTGGAGACCACAGTTTCGTCAAGGTCACCAAGGCTGGGAAAAAAAAAGAAGCATCA
  ACTGTCCAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001301745
• Insert Size: 1272 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001301745.1, NP_001288674.1
• RefSeq Size: 1783 bp
• RefSeq ORF: 1272 bp
• Locus ID: 381308
• UniProt ID: P0DOV1
• Cytogenetics: 1 H3
• Gene Summary: The protein encoded by this gene is a member of the interferon-regulated 200 family of proteins, which contain an N-terminal pyrin domain that is proposed to function in cell death and a partially conserved 220 amino acid domain. Expression of this protein in embryonic stem cells is critical for the DNA damage response and regulation of cell survival. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2014]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.