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Wdr81 (NM_138950) Mouse Untagged Clone
CAT#: MC225052
Wdr81 (untagged) - Mouse WD repeat domain 81 (Wdr81), (10ug)
"NM_138950" in other vectors (2)
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Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Symbol | Wdr81 |
| Synonyms | BC054822; Gm883; mFLJ00182; nur5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC225052 representing NM_138950
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCAGGGGAGCAGAAGGCGGAAAGTGGTTCTTACAGCAGGGTCCGAGGGCTGGTCCCCTTCCTCAG GACCTGACATGGAGGAGCTGCTCCGGAGCGTGGAGAGAGATCTGAACATTGATGCCCGGCAGCTGGCCCT GGCGCCGGGGGGCACTCATGTAGTGGCCCTAGTGTCCACGCGTTGGCTGGCTAGTCTCCGGGAGCGCCGA CTGGGACCCTGTCCCCGGGCTGAGGGCCTGGGTGAAGCAGAAGTCAGGACTTTACTGCAACGTTCGGTAC AGAGGCTGCCCCCAGGCTGGACTCGAGTGGAGGTGCATGGGCTGCGGAAACGGAGACTGTCCTACCCGCT GGGTGGAGGCGTGCCCTTTGAGGAGGGGTCCTGTAGCCCTGAAACTCTCACTCGGTTCATGCAGGAGGTG GCTGCCCAGAATTACCGGAACCTGTGGCGCCATGCATACCACACTTATGGACAGCCTTACAGCCACAGCA CTGCCCCCTCAGCTCTACCTGCCCTAGACTCTATACGACAAGCTCTCCAGAGGGTGTATGGATGCACCTT CTTGCCAGTGGGTGAATCCATCCCATGTCTATCAAATGTCAGGGATGGGCCCTGCCCCTCTCGGGGCAGC CCTGCCTGCCCCAGCCTTTTGCGAGCTGAGGCTTTGCTGGAGTCGCCCGAGATGCTCTATGTGGTACACC CTTATGTGCAATTCTCCCTGCATGATGTAGTTACCTTCAGCCCTGCCAAGCTGACCAACAGCCAAGCCAA GGTGCTCTTTCTTCTCTTCCGTGTTCTGAGGGCCATGGATGCCTGTCACCGCCAGGGGCTGGCCTGTGGG GCTCTGTCTTTGCACCACATTGCTGTAGACGAGAAGCTATGCAGTGAGCTCCGGCTGGACCTGAGCGCTT ACGAGATGCCTTCCGAGGATGAAAACCAGGAGGGCTCTGAAGAGAAAAATGGGACAGGCATTAAGTCTGA AAAAGAGGGGGAAGGGAGAACTGAGTGTCCCACCTGCCAGAAAGAACTTCGGGGCCTTGTGCTAGACTGG GTCCATGGCCGAATCAGCAACTTCCACTACCTCATGCAGCTGAATCGGTTGGCAGGTCGACGGCAGGGGG ATCCCAACTATCACCCAGTGCTGCCCTGGGTGGTGGACTTTACCACACCTTATGGGCGCTTCCGAGACCT TCGTAAATCCAAGTTCCGACTCAACAAGGGAGATAAGCAATTGGACTTCACCTATGAGATGACCCGGCAG GCATTTGTTGCAGGTGGTGCAGGAAGTGGGGAGCCACCCCATGTTCCTCACCACATCTCTGACGTGCTCT CTGACATCACGTACTATGTATACAAGGCCCGTCGCACACCGCGCTCGGTGCTCTGTGGACATGTCCGAGC GCAGTGGGAACCCCACGAGTATCCTGCCACCATGGAGCGGATGCAGACCTGGACACCGGATGAGTGCATA CCCGAGTTCTACACGGACCCCTCTATCTTTTGCTCTATCCACCCTGACATGCCCGACCTGGATGTGCCGG CCTGGTGCAGTTCTAACCAGGAATTTGTGGCTGCCCATCGAGCCCTCCTGGAGAGCTGGGAGGTGTCCCA AGACCTGCATCACTGGATTGATCTTACCTTTGGCTACAAACTCCAGGGCAAAGAAGCTGTGAAGGAGAAG AATGTGTGTCTGCACCTGGTGGACGCTCACACCCATCTGACCAGCTATGGCGTGGTACAGCTATTTGATC AGCCACACCCCCAACGCCTGGCTGGATCTCCTGCCCTGGCCCCTGAACCTCCACTCATCCCCCGGCTGTT GGTCCAGCCTATTCGGGAGGCCACAGGCCAGGAGGACATTTCAGGACAACTTATAAATGGTGCGGGCAGG CTTGTCGTAGAGGCCACTCCATGTGAGACTGGCTGGACTAGAGATAGGCCTGGGACAGGAGAAGATGATT TAGAACAGGCTACAGAAGCTCTGGATTCCATCTCCCTCCCCGGGAAAGCAGGTGACCAGCCAGGCTCTTC CTCCAGTCAAGCATCACCTGGCCTGTTGTCTTTTTCTGCACCCTCGGGGTCTCGACCAGGCCGTAGGAGC AAAGCTGCCGGGTTGGACCCTGGGGAGGGTGAAGAGGGCAAGATTGTCCTTCCAGAGGGCTTCAGTCCCA TACAGGCCTTGGAAGAGCTGGAGAAAGTGGGTAACTTCCTGGCCAAAGGCCTAGGGAGCCAGTTGGAGGA GCCTGAAAAGCCTCACGCCCAGCCACCTGTGCACCTGCAGAGCCTCTTCCATCGAGACATGCAGGTCCTG GGTGTCCTGTTGGCTGAGATGGTGTTTGCCACCAGGGTCCGGATACTGCAGCCTGATGCACCTTTGTGGG TACGCTTTGAGGCTGTTCGGGGTCTCTGCATACGCCACTCCAAGGACATCCCCGTGTCTCTGCAGCCTGT GCTAGACACACTCCTACAGCTGAGCGGACCCAAAAGTCCCATGGTGTCGAAGAAGGGCAAGCTAGACCCA CTGTTTGAGTATAGGCCGGTTTCCCAGGGATTACCCCCACCCAGCCCAGCCCAGCTCCTCAGCCCCTTCA GCTCCGTGGTCCCCTTCCCTCCATACTTCCCAGCACTGCACAAGTTCATTCTTTTATATCAGGCCCGGCG TGTGGAGGATGAGGTCCAGGGTCGGGAGCTGGCGTTTGCTCTGTGGCAGCAGCTGGGTGCGGTGTTAAAT GACATCACTCCCGAGGGCTTAGAGATCCTCCTGCCTTTCGTGCTGTCGCTCATGTCTGAGGAGCACACGG CTGTGTACACAGCCTGGTACCTATTTGAACCCGTTGCCAAGGCCCTGGGCCCCAAAAATGCCAACAAGTA CCTCCTGAAGCCTCTCATCGGTGCCTATGAGAGCCCCTGCCGCCTGCATGGCCGCTTCTACCTGTACACC GACTGTTTTGTGGCCCAGTTGGTGGTGCGGCTGGGCTTGCAGGCCTTCCTCACCCACCTGCTGCCCCATG TCCTCCAGGTACTGGCTGGGGTGGAGGCTTCCCAGGAGGAGGGCAAAGGCCTGGTCGGGACCACTGAGGA TGAGGAAAGTGAGCTCCCGGTGTCCGGGCCTGGCTCCTGTGCCTTTGGGGAAGAGATTCAGATGGATGGG CAGCCGGCTGCTTCCTCAGGACTGGGGCTCCCAGACTACAGGTCGGGCGTCAGCTTCCATGACCAGGCCG ACCTGCCGGACACGGAGGACTTCCAAGCTGGACTCTACGTGGCTGAATCTCCACAGCCCCAGGAGGCTGA GGCCGTGAGCCTGGGCCAGCTGAGTGATAAGAGCAGTACCAGCGAAGCCTCCCAGGGCGAGGAGAGGGGT GGGGATGATGGCGGTGCCCCTGCGGACAAGAACAGCGTCAAGTCAGGGGACAGCAGCCAGGACTTGAAGC AGAGCGAAGGCTCTGAGGAAGAGGAGGAGGAGGAAGGCTGTGTGGTGTTGGAGGAGGACCAGGAGGATGA AGTCACGGGAACATCCGAGCTCACTCTGTCTGACACGATGCTGTCCATGGAGACGGTGGTGGCTCCTGGT GATGGGAGAGACAGAGAAGAGGAAGAGGAGCCGCTGACAGAGCAGACAGAAGGCAAAGAACAAAAGATCC TCCTTGATACAGCCTGCAAGATGGTCCGCTGGCTGTCTGCCAAGCTTGGCCCCACAGTAGCCTCTCGCCA TGTGGCCCGGAACCTGCTGCGCCTGCTGACATCTTGTTATGTTGGGCCCACTCGACAGCAGTTCACCGTC AGCAGTGATGACACCCCTCCACTGAATGCCGGCAACATCTACCAGAAGAGGCCAGTCCTAGGTGACATCG TGTCGGGGCCTGTGCTCAGCTGCCTCCTCCACATTGCCTACCTGTATGGAGAACCCGTTCTCACCTACCA GTACCTGCCCTACATCAGCTACCTGGTAGCCCCAGGGAGCAACTCAAACCCCAGCCGACTGAACAGCCGC AAGGAGGCCGGGCTGCTGGCAGCGGTGACACTGACGCAGAAAATCATCGTATACCTCTCTGACACGACCC TCATGGACATTCTGCCCCGCATTAGCCACGAGGTCTTGCTGCCTGTGCTTGGCTTCCTCACCTCCTTCGT CACAGGGTTCCCCAGTGGGGCCCAGGCCCGGACTGTCCTATGCGTGAAAACCATCAGTCTCATCGCCCTC ATCTGCTTGCGCATCGGGCAGGAGATGGTCCAGCAGCACCTGAGTGAGCCAGTGGCCACCTTCTTCCAAG TCTTCTCTCATCTGCATGAGCTTCGGCAGCAGGATCTGCCACTGGATCCTAAGGGCTGTACTGAGGGCCA GCTGCCAGAGGCGACCTTCTCTGATGGGCAGCGACGACCAGTGGACCCCACCCTGCTGGAAGAGCTGCAG AAGGTGTTCACCCTGGAAATGGCGTACACAATCTACGTACCTTTCTCCTGCCTGTTGGGTGACATCATCC GGAAAATCATCCCCAACCATGAGTTGGTCGGGGAGCTGGCAGGGCTCTATCTGGAAAGCATGAGCCCGAG CTCTCGAAACCCAGCCAGCATGGAACCCACCATGGCTAGTGCCGGCCCTGAATGGGACCCTCAGAGTGGG AGCTGTCTCCAGGACGATGGCCACTCAGGGACCTTTGGGAGTGTCCTGGTTGGAAATCGCATCCAGATCC CTGACTCTCAGCCCCAGAGTCCTGGGCCACTGGGCTCCCTCTCTGGAGTGGGTAGTAGCGGAGGCCTCAG CAACAGGAATGAAGACAACGCCCTGAAGCGGGAGCTGCCTCGGAGTGCCCATGGGCTGAGCGGGAACTGG CTGGCGTACTGGCAGTACGAGATCGGTGTGAGCCAGCAGGATGCCCACTTCCACTTCCACCAGATCCGCC TGCAGAGCTTCCCAGGGCACACGGGGGCCGTCAAATGCGTGGCCGCCCTGAGCAGTGAAGACTTCTTTCT GAGTGGCAGCAAGGACCGGACTGTGCGCCTCTGGCCGCTGTACAACTATGGGGACGGGACCAATGAGACG GCTTCCCGCCTCATCTATGCCCAGCACCGCAAAAGCGTCTTCTACGTGGGCCAGCTTGAGGCCCCGCAGT ATGTGGTGAGCTGTGATGGGGCAGTGCACGTCTGGGACCCCTTCACAGGAAAGACCCTTCGCACAGTGGA TCCTTCAGACAGCCGGGTGCCCCTGACGGCTGTGGCTGTCATGCCTGCCCCACACACCAGCATCACCATG GCCAGCTCCGACTCCACTCTGCGCTTTGTGGACTGCAGGAAGCCAGGCTTGCAGCATGAGTTCCGACTGG GTGGAGGGCTGAACCCTGGGCTTGTTCGCTCGTTGGCCGTCAGCCCCAGTGGCCGGAGTGTTGTGGCTGG CTTCTCCTCGGGCTTCATGGTGCTCCTAGATACCCGCACGGGCCTGGTTCTACGAGGCTGGCCAGCCCAT GAAGGGGACATTCTACAGATCAAGGCTGTAGAGGGCAGCGTGCTCATCAGCTCCTCTTCCGACCATTCCT TGACTGTTTGGAAGGAGCTGGAACAGAAGCCCACGCACCACTACAAGTCAGCGTCCGACCCAATCCACAC CTTTGACCTGTACGGCAGCGAGGTGGTCACCGGCACTGTAGCCAACAAGATTGGTGTCTGTTCCCTGCTT GAGCCACCCTCTCAGGCCACCACAAAGCTCAGTTCCGAGAACTTCCGTGGCACGCTCACTAGTCTGGCTT TGCTGCCCACGAAACGCCACCTCCTGCTGGGCTCGGACAATGGCATCATCCGCCTCCTGGCATAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-RsrII |
| ACCN | NM_138950 |
| Insert Size | 5805 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_138950.2, NP_620400.2 |
| RefSeq Size | 6976 bp |
| RefSeq ORF | 5805 bp |
| Locus ID | 192652 |
| UniProt ID | Q5ND34 |
| Cytogenetics | 11 B5 |
| Gene Summary | Functions as a negative regulator of the PI3 kinase/PI3K activity associated with endosomal membranes via BECN1, a core subunit of the PI3K complex. By modifying the phosphatidylinositol 3-phosphate/PtdInsP3 content of endosomal membranes may regulate endosome fusion, recycling, sorting and early to late endosome transport. It is for instance, required for the delivery of cargos like BST2/tetherin from early to late endosome and thereby participates indirectly to their degradation by the lysosome. May also play a role in aggrephagy, the macroautophagic degradation of ubiquitinated protein aggregates. In this process, may regulate the interaction of SQSTM1 with ubiquitinated proteins and also recruit MAP1LC3C (By similarity). May also be involved in maintenance of normal mitochondrial structure and organization (PubMed:23595742).[UniProtKB/Swiss-Prot Function] |
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