Marf1 (NM_001081154) Mouse Untagged Clone

CAT#: MC224895

Marf1 (untagged) - Mouse RIKEN cDNA 4921513D23 gene (4921513D23Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Marf1
• Synonyms: 4921513D23Rik; BC031575; C87306; E030019O05; Lkap; mKIAA0430
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224895 representing NM_001081154
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGGGAAAGGAACTGAGAACCCTTGCAGCAGAACACTTGGATGGTTTCACCAAGATAATGATGCTA
  AGCCGTGGCTTTGGAAATTCTCTGGTTGCTTTTCTCGTCCCGAGCAGACATTGCCTCTTAGTTCCCAAAC
  GAAAGAGTACATGGAGAACAAGAAAGCTGCTGTGGAGTTAAAGGATATACCATCTCCCCTTCATGTTGGC
  TCTAAATTTTTCCCAGCAGTCCCACTTCCTGATATTCGTTCTCTTCAGCAACCTAAAGTACAGCTCTCAG
  CTATTCCCAAAGTAAGCTGCTGTGCTCATTGCCCTAATGAACCCTCCACTTCACCCATGCGTTTTGGTGG
  TGGCAGTGGCAGTAGTGGAGGTTCTGGGAGCTTGATTCACACGGGCTCACTCTTAGACTCACCAAGCACT
  GGGACAGTCACGTGTCAAGTAGGGTCAGGTTTTGCTTTCCAGTCCGTGTCTTCACTGCAGAATGCCTCCA
  CTAGGAACAACTTGGTGGGCCTTTCAAGTGACTTCCCCAGCATGTGTGTAGAGAGTAACCTACCTTCCTG
  CAAGCACCTGTCGTGTTGTGGGAAGCTCCATTTCCAGTCATGTCACAGTAATGTACACAAGCTGCATCAG
  TTCCAGAATCTTCAGGGCTGTGCCTCTGCTGGCTATTTCCCCTGTTCCGATTTCCCAAGTGGGGCTCCTG
  GGCATTTGGAAGAGCGCCTTTCCCATTCAGAGCTTACACCTCACTTGTGCACCAACTCTCTTCACCTAAA
  TGTGGCACCTCCGGTTTGTTTAAAGGGCTCACTTTACTGTGAAGACTGTCTAAACAAGCCAGCAAGAAAT
  AGTATAATTGATGCTGCTAAAATCTGGCCAAATATACCACCTCCAAGTACTCAGCCTGCACCTCCAGCTA
  TTCCTGTGTGTAATGGCTGTGGAACAAAAGGCATGGAAAAGGAGACCTCATTGTTGTTGGCAACCAGTCT
  AGGCAAAACTGCTTCGAAATTTGGGTCACCAGAAGTTGCAGTCACTGGACAGGTGCTGGAGACCCTCCCC
  CCCATTGGCGTTTTCTGGGATATTGAAAACTGCTCAGTCCCCTCTGGCCGGTCAGCAACAACTGTTGTAC
  AGAGAATCCGTGAGAAGTTTTTTAGAGGACACAGAGAAGCAGAGTTTATCTGTGTGTGTGACATCAGTAA
  AGAAAACAAGGAAGTTATTCAAGAGCTGAATAATTGTCAGGTAACCGTTGCTCACATAAATGCTACTGCA
  AAGAATGCTGCTGATGATAAACTCCGTCAGAGCCTGCGAAGGTTCGCAAACACGCACACTGCTCCAGCTA
  CTGTGGTTCTTGTGTCAACTGATGTCAATTTTGCGTTGGAACTCAGTGATCTTAGGCACCGGCACGGTTT
  CCACATCATTTTGGTACATAAAAACCAGGCCTCTGAAGCCTTGTTACATCATGCTAACCAGCTGATCAGA
  TTTGAAGAGTTCATTTCTGATCTCCCCCCTCGGTTACCACTGAAGATTCCTTGCCACACTCTACTCTATG
  TTTATAACCTGCCAGCAAATAAAGATGGCAAGAGCATCAGCAACAGGCTCAGGCGCCTCTCTGATAACTG
  TGGTGGCAAGGTGCTGAGTATCACAGGCTGCAGCGCAATTCTCCGCTTCATAAACCAGGACAGTGCAGAG
  CGGGCCCAGAAGCGAATGGAAAATGAAGATGTCTTTGGCAACAGGATCATCGTGTCCTTTACTCCAAAAC
  ATAGAGAATTCTTTGAAGCCAAGAGTTCAAATGCTATTGCCGACAAAGTGAAGTCTCCCAAAAAAGTTAA
  GAACACAAAATTGTGCCTCATCAAAGACACAAGTGAACAGTCACCCAGTGTCAAAGCCATGCCAGGGAAA
  GTGTTGCAGGCAAATTCTGGATCTGCTACAAAAACCACAAACATTAAAAGTTTACAGGAGCTGTGCCGAA
  TGGAGTCAAAGTCTGGCAATAGAAACAGTGACCATCAGCAAGGCCATGGGAGGCTGGCAGCACTGCCTAA
  CAGTGGTCCAACTGCTTCAGTGCCCATCGTGAAGAACACAGGTGTGACAGAGCCACTTTACAGAAGCAGT
  CAGAAAAAAGAGAACCCCAGTTCCCAGAGCACTGTCAACTCTCCTGTAGAGAAGAAGAAGAGAGAGGAGA
  CTGTCTTCCAAGTAAGCTACCCATCCGCATTCAGCAAGCTGATCGCCTCAAGGCAAGTCAGCCCTCTGCT
  CACCTCTCAGTCTTGGTCTCCGAGGAGTATGTCTCCAAACCTTTTGAATAGAGCCTCACCACTTGCTTTC
  AACATTGCCAATCCCAGCAGCGGTGCTGACTGCCCAGACCCATTTGCAAATGGTGTTGACATCCAGGTCA
  GCAATGTGGACTACAGGCTGTCCCGGAAGGAGCTGCAGCAGCTCCTCCAGGAAGCCTTTTCCAAGCATGG
  CCAGGTGAAGAGTGTTGAGCTCAGTCCCCATACTGATTACCAGCTCAAGGCCATTGTGCAGATGAGAAAC
  CTGCACGAAGCAATCTGTGCAGTGAACAGCCTCCATAGGCACAAAATTGGCAGCAAGAAGATACTGGTCT
  CACTCTCCACAGGAGCTGCCAACAAGTCACTCTCTTTACTAAGCACAGAAACAATGTCTATTCTTCAGGA
  TGCTCCTGCCTGTTGCTTGCCTCTCTTTAAATTTATAGACATCTATGAAAAGAAGTATGGACACAAGTTA
  AATGTGTCTGATTTATATAAATTAACAGACACTATTGCAATCCGTGAACAGGGAAACGGGCGATTGGTGT
  GTCTCTTACCTAGTAATCAGGCCCGCCAGAGCCCCCTGGGCTCTTCCCAGTCACATGATGGCTCCTCCTC
  AAACTGCAGCCCAGTTCTGTTTGAAGAGTTGGAGTATCACGAACCTGTCTGCAAGCAGCACTGCTCTAAC
  AAGGACTTCAGTGAACTTGTGTTTGATCCAGACTCTTACAAGATTCCTTTTGTGGTTCTCTCTTTGAAAG
  TGTTCGCACCTCAAGTTCATAGTCTTCTGCAGACCCACCAGGGCACTGTGCCTTTGCTAAGCTTTCCAGA
  TTGCTATGCTGCGGAGTTTGGTGACCTAGAAATTACGCAAGACAGCCACAAGGGTGTCCCCTTAGAGCAC
  TTCATCACCTGCATTCCAGGAGTGAACATTGCCACTGCTCAGAATGGCGTCAAAGTAGTGAAGTGGATTC
  ACAACAAGCCCCCACCTCCCAACACTGACCCTTGGCTTCTTCGTTCTAAAAGTCCTGTGAGAAATCCCCA
  GCTGATTCAGTTCAGCAGAGAGGTCATTGATTTACTGAAGACCCAGCCATCGTGTATACTACCCGTCAGC
  AATTTCATCCCTTCATACCATCACCACTTTGGAAAGCAGTGCAGGGTTTCAGACTATGGTTATTCCAAGC
  TGATTGAACTCCTAGAAGCTGTGCCTCATGTGTTGCAGATTCTTGGTATGGGCTCCAAGCGTCTGATGAC
  CCTTACTCACAGGGCCCAGGTGAAGCGCTTTACTCAGGATTTACTAAAACTTCTTAAGTCCCAGGCCAGC
  AAGCAGGTCATCGTGAGAGACTTTTCACAGGCTTATCATTGGTGTTTCTCAAAGGACTGGGATGTCACGG
  AATATGGTGTTTGTGACTTGATTGATATAATATCAGAGATCCCAGACACAACTATTTGCTTGTCCCAACA
  AGATGACGACATGGTCATTTGTATCCGAAAAAGAGAACGAACTCAGGATGAAATAGAAAGGACAAAGCAG
  TTCTCCAAAGATGTTGTGGACTTGCTGCGCCACCAGCCCCATTTCAGAATGCCCTTCAACAAGTTCATTC
  CCTCTTACCACCACCACTTTGGCCGACAGTGTAAACTTGCATACTATGGATTTACCAAGCTGCTTGAACT
  TTTGGAAGCCATACCTGAGATTTTACAAGTGTTGGAATGTGGAGAAGAAAAAATCCTGACTTTGACTGAA
  GTGGAGCGATTCAAAGCTCTAGCCGCCCAGTTTGTTAAGCTTCTGCGGTCCCAAAAGGGTAACTGCCTTA
  TGATGACAGATCTGTTTACAGAATATGCTAAAACTTTTGGTTATACATTTCGTCTCCAAGACTATGATGT
  CAGTTCAGTCTCAGCTTTGACACAGAAGCTCTGCCATGTTGTAAAGGTTGCTGATATGGAGTCTGGTAAA
  CAGATCCAGCTGATCAATAGGAAGTCTCTGCGGTCTCTCACTGCTCAGTTGCTGGTCTTGTTGATGTCTT
  GGGAAGGGGATGCTTATCTTCCTGTTGATGAGCTCAGGAGACATTATGAAACCACTCACAGCACTCCTCT
  CAACCCCTGTGAATATGGATTCATGACCCTGACTGAGCTACTGAAGAGTCTGCCGTACTTAGTAGAGGTT
  TGTACGAACGATAAGGCAGAAGAGTATGTGAAGCTCACAAGCCTGTACTTGTTTGCAAAGAATGTGAGGT
  CTTTGCTTCATACCTATCACTACCAGCAGATTTTTCTTCATGAATTTTCCATGGCCTATAACAAATATGT
  TGGAGAAACACTACAGCCCAAAACTTATGGCTACAGTAGTGTGGAGGAATTACTGGGAGCCATCCCACAG
  GTGGTCTGGATAAAAGGACATGGTCATAAGAGAATTGTAGTGTTAAAAAATGACATGAAAAACCGCCTAA
  GCTCATTTGACCTTTTCTCTGTCAATCATGAAGACCAGCCTGCAGTGAGCAGGCAGATCCTGGCGGTGCC
  AGAATCCCATCTAGCCTCAGAACTCCAGCTCCGAACAGGACCCAGCCAGATGGAGCAGGAGCTTCTGCAC
  CTGGCCAGCAGCTCCCCCGTTGACCTGCTCTGTGCTCCTGTTCCTTCATGCCTGCCATCCCCTCAGCTAA
  GACCAGATCCTGTCATCCTGAAGCCCGCTGATCTCATTCAGTTTGAGGAACACCCTCAAGAGCCTTTGGG
  AGTTTTAGTTTTAAACCAAGAAGAAAAATCAGAAGTTCCACTACCAGTGCAAAAGGGAAACCTGTCCTGT
  GACTCCTCACCCAGCAGCCCAGCAGCTTCTCCTGCTCCTCCTGGCCCCTCCTCAGAAGCCCCCAGGCCAC
  TTTTCAGCAAGGATGCAGTGGAAAGCCCAGCCAAAAAGCAGCCCAAAAATAGAGTCAAATTGGCAGCCAA
  CTTTTCTTTTGCACCTGTAACCAAGCTTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001081154
• Insert Size: 5211 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081154.2, NP_001074623.1
• RefSeq Size: 7765 bp
• RefSeq ORF: 5211 bp
• Locus ID: 223989
• UniProt ID: Q8BJ34
• Cytogenetics: 16 A1
• Gene Summary: Essential regulator of oogenesis required for female meiotic progression to repress transposable elements and preventing their mobilization, which is essential for the germline integrity. Probably acts via some RNA metabolic process, equivalent to the piRNA system in males, which mediates the repression of transposable elements during meiosis by forming complexes composed of RNAs and governs the methylation and subsequent repression of transposons. Also required to protect from DNA double-strand breaks.[UniProtKB/Swiss-Prot Function]