Dot1l (NM_199322) Mouse Untagged Clone

CAT#: MC224665

Dot1l (untagged) - Mouse DOT1-like, histone H3 methyltransferase (S. cerevisiae) (Dot1l), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dot1l
• Synonyms: A630076O07; AW907654; Dot1; KMT4; mDot1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224665 representing NM_199322
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGAGAAGCTGGAGCTGAGGCTCAAGTCGCCTGTGGGCGCCGAGCCCGCCGTCTACCCGTGGCCGC
  TGCCGGTCTACGACAAGCACCATGATGCTGCTCATGAGATTATTGAGACTATCCGGTGGGTCTGCGAGGA
  AATCCCAGATCTCAAGCTGGCCATGGAGAACTATGTCCTGATCGACTACGACACCAAAAGTTTTGAAAGC
  ATGCAGCGGCTGTGTGACAAATACAACCGGGCCATCGACAGCATCCACCAGCTGTGGAAGGGCACTACAC
  AGCCCATGAAGCTGAATACTCGGCCGTCCAATGGACTTCTGCGGCACATCCTGCAGCAGGTATATAACCA
  CTCTGTCACTGACCCGGAGAAGCTTAACAACTACGAGCCCTTCTCCCCTGAGGTGTATGGGGAGACCTCC
  TTTGACCTGGTGGCCCAGATGATTGATGAGATCAAGATGACAGAGGATGACCTGTTTGTCGACCTGGGCA
  GTGGTGTGGGGCAGGTTGTCCTTCAGGTTGCTGCGGCCACCAACTGCAAACATCACTACGGAGTGGAGAA
  AGCGGACATCCCAGCCAAGTACGCAGAGACCATGGACCGAGAGTTCAGGAAGTGGATGAAATGGTATGGA
  AAAAAGCATGCAGAATACACACTGGAACGAGGTGACTTCCTCTCAGAGGAATGGAGGGAACGGATCGCCA
  ACACGAGTGTTATATTTGTGAATAACTTTGCCTTTGGTCCTGAGGTGGATCACCAGCTGAAGGAGCGATT
  CGCAAACATGAAGGAAGGCGGCAGAATCGTATCCTCAAAGCCCTTTGCCCCTCTGAACTTCAGGATCAAC
  AGCAGGAACTTGAGTGACATTGGCACCATCATGCGTGTGGTGGAGCTGTCGCCCCTGAAGGGCTCTGTGT
  CGTGGACTGGCAAGCCTGTCTCCTACTATCTGCACACCATTGACCGCACCATACTTGAAAACTATTTTTC
  TAGTCTGAAAAATCCAAAACTCAGGGAGGAGCAGGAGGCAGCTAGGCGAAGACAGCAGCGAGAGAACAAG
  AGCAATGCGACCACGCCCACCAAAGTCCCTGAGAGCAAAGCAGCTGCCACTGAGGCCCCCGCGGATTCTG
  GTGCTGAGGAAGAAAAATCAGGTGTGGCTACTGTCAAAAAGCCATCTCCCTCCAAAGCCCGGAAGAAGAA
  ACTGAACAAGAAAGGGAGAAAGATGGCTGGCCGGAAACGTGGGCGGCCCAAGAAAATGAGTGCTGCCAGT
  GCCGAACGCAAGTCTAAGAAGAGCCAAAGCACACTGGATCTCCTGCACTCTCCGCCCCCAGCCCCACCCT
  CAGCCTCACCCCAGGATGCGTACAGGGCACCTCACAGCCCATTCTACCAGCTACCTCCAAGCACGCAGCT
  GCACTCACCCAACCCACTGCTAGTGGCACCCACCCCACCTGCGCTGCAGAAGCTTTTAGAGTCCTTCAGA
  ATCCAGTACCTGCAGTTCCTGGCTTATACAAAGACCCCGCAGTACAAGGCTAACCTACAGCAGCTTCTGG
  ACCAGGAGAAGGAGAAGAACACACAGCTCCTGGGCACAGCCCAGCAGCTCTTCGGCCATTGCCAGGCCCA
  AAAGGAAGAAATCCGCAGGCTGTTCCAGCAGAAGCTGGATGAGTTGGGCGTGAAGGCGCTGACCTACAAT
  GACCTGATTCAGGCCCAGAAGGAAATCTCTGCCCACAACCAGCAGCTGCGGGAACAGTCGGAACAGCTGG
  AGAAGGACAACAGCGAGCTTCGGAGCCAGAGCCTGCGGCTGCTCAGGGCCCGGTGTGAGGAGCTGAGGCT
  TGACTGGTCCACACTGTCCTTGGAGAACCTGCGGAAAGAGAAGCAGGCCCTGAGGAGCCAGATCTCAGAG
  AAGCAGCGGCACTGCCTGGAGCTGCAGATCAGCATCGTGGAGCTCGAGAAGACCCAGCGCCAGCAGGAGC
  TCCTGCAGTTAAAGTCCTGTGTACCACCAGATGATGCTTTGTCTTTGCATCTGCGTGGCAAGGGTGCCCT
  GGGCCGAGAGCTGGAGGCTGATGCTGGGCGCTTACGCCTTGAGCTGGACTGTGCCAAGATCTCCCTGCCA
  CACCTCAGCAGCATGAGCCCTGAGCTGTCCATGAACGGCCATGCTGCCAGCTATGAGCTCTGCAATGCAG
  CTAGTCGGCCATCATCCAAGCAGAACACACCCCAGTACCTGGCCTCTCCCTTGGACCAGGAGGTCGTACC
  TTGTACCCCTAGCCACAGTGGCCGGCCTCGGCTAGAAAAGCTGTCTGGCCTGGCTTTGCCTGATTACACC
  CGGTTGTCACCTGCCAAGATTGTGTTGAGGCGGCACCTGAGCCAGGACCACACTGGGGCTAGCAAAGCAG
  CTACCAGTGAGCCACACCCTCGGCCAGAGCACCCCAAGGAGAGCAGCCTTCCCTACCAGAGCCCTGGCTT
  GTCCAACAGCATGAAGCTCAGCCCCCAGGATCCACCACTGGCCTCCCCGGCAACCTCGCCCCTCACCTCC
  GAGAAAGGTAGTGAGAAGGGTGTGAAGGAGCGTGCCTACAGCAGCCACGGGGAGACCATCACCAGCCTAC
  CTGTTAGCATTCCACTCAGCACAGTGCAGCCCAACAAGCTGCCGGTCAGCATCCCACTGGCCAGCGTGGT
  GCTGCCCAGCCGTGCTGAGAGGGCGAGGAGCACTCCCAGCCCTGTGCCACAGCCCCGGGACTCTTCAGCC
  ACACTTGAGAAGCAGACTGGTGCTTCTGCCCATGGTGCAGGGGGTGCTGGAGCAGGAAGCAGGAGCCTCG
  CTGTGGCGCCCACAGGTTTCTATGCTGGTTCGGTGGCCATCAGTGGGGCCCTGGCCAGCAGCCCAGCACC
  TCTGGCCTCTGGAATGGAATCTGCTGTTTTTGATGAATCCTCTGGCCCCAGCAGCCTCTTTGCCACCATG
  GGGTCTCGCAGCACACCACCACAACACCCACCTCTCCTGTCGCAGTCCCGCAACTCGGGTCCTGCCTCTC
  CTGCCCACCAACTCACAGCCAGTCCCCGCCTGAGTGTGACCACTCAGGGCTCGCTGCCAGACACCAGCAA
  AGGGGAACTGCCTTCTGATCCTGCCTTCTCAGACCCAGAGAGCGAAGCAAAAAGGAGGATTGTGTTCAGC
  ATTTCAGTTGGTGCCAGCTCCAAGCAGTCACCTTCCACCAGGCATAGCCCCTTGACTTCTGGCACCCGAG
  GGGACTGTGTGCAGAGCCACGGACAGGACAGTCGTAAGCGCAGCAGAAGGAAGCGTGCATCAGCTGGAAC
  ACCTAGCCTCAGCACGGGTGTGTCCCCCAAGCGCCGGGCTCTGCCAACTGTTGCTGGCCTCTTTACACAG
  TCCTCGGGGTCTCCTCTTAATCTCAACTCCATGGTCAGCAACATCAACCAGCCACTGGAGATCACAGCCA
  TCTCATCCCCTGAGAGCTCCCTGAAGAGCTCCCCCACCCCATACCAGGACCATGACCAGCCTCCGGTGCT
  CAGGAAGGAACGGCCTCTAGGACTAACTAATGGGGCCCACTACTCGCCACTGACCTCAGATGAGGAGCCA
  GGCTCTGAGGATGAGCCCAGCAGTGCCCGAATTGAGAGAAAAATTGCAACAATCTCTTTAGAAAGCAAAT
  CTCCCCCGAAGACACTGGAAAATGGTGGTGGCTTGGTGGGAAGGAAGTCAGCCCCTTCGAGCGAGCCTAT
  CAACAGCAGCAAATGGAAGTCTACCTTCTCTCCTATCTCTGACCTCGGCTTGGCCAAGGCTGTGGACAGT
  CCGCTACAGGCTGGCTCTGCCCTGAGCCACAGCCCCCTGTTCTCTTTCCGGCCGTCCCTGGAGGAGCCTG
  CTGCTGAGGCCAAGCTCCCCACTCACCCAAGGAAAAGCTTTGCGGGCTCTCTGGGTGCAGCCGAGGGGCC
  AAGCCCTGGCACCAACCCTCCCAACGGCCTGGCCTTCAGTGGGGGCCTTGCTGCAGACCTCGGTTTACAC
  AGCTTCAATGATGGTGCTTCCCTCTCCCACAAGGGCCCGGATGTGACTGGCCTGAGTGCCTCCCTGAGCT
  TCCCATCCCAGAGGGGCAAGGACAGTACCACAGAGGCCAACCCTTTCCTCAGCAGGCGGCAGCCCGAGGG
  CCTGGGTGGCTTGAAGGGTGAGGGCAACGCAAACAAGGAGTCAGGAGAGAGCCTGCCCCTGTGCGGGCCC
  TCGGACAAGGCCTCACTGCCACATGGCAGCAGGGCCAGCAAAGGTCGTGACCGTGAGCTGGACTTCAAGG
  GCGGCCACAACCTCTTCATCTCTGCTGCAGCCGTGCCTCCAGGTGGCCTCCTTGGTGGCCCTGGTCTTGT
  AACTGTGGCTTCTTCTGCGGGCAGTGCAACACCCACTGCCCAGGCTCCCCGGCCTTTCCTGAGCACCTTT
  GCCCCCGGGCCCCAGTTTACTCTGGGCCCCATGTCCCTGCAGGCCAACCTGGGCTCTGTGGCCGGCTCCT
  CTGTGCTGCAGTCCTTGTTCAGCACTGTGCCAGCTGCTGCAGGCCTGGTGCATGTGTCGTCCACTGCGAC
  CCGATTGACCAACTCACACACCATGGGCAGCTTCTCTTCTGGGGTGGCCGGCGGAACCGTTGGAGGTAAT
  TAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_199322
• Insert Size: 4623 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_199322.1, NP_955354.1
• RefSeq Size: 5694 bp
• RefSeq ORF: 4623 bp
• Locus ID: 208266
• Cytogenetics: 10 C1