Zcchc6 (NM_153538) Mouse Untagged Clone

CAT#: MC224527

Zcchc6 (untagged) - Mouse zinc finger, CCHC domain containing 6 (Zcchc6), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Zcchc6
• Synonyms: 6030448M23Rik; AA420405
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224527 representing NM_153538
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGATACAGCAAAACCTTACTTTGTGAAGCGCACTAAAGACCGTGGAATTATTGATGATGATGATT
  TCAGAAGGGGCCATCCCCAACAAGATTATTTAATAATGGATGATTATGCTAAAGGCCATAGCAGTAAAAT
  GGAAAAGGGCCTTCCAAAAAAGAAGATATCACCCGGGAACTATGGGAACACCCCCAGGAAAGGACTGTAT
  GGGGTTTCCAGCAATCCATATGCATTTAAAAACCCAATTTACAGTCAGCCTGCTTGGATGAATGATAACC
  ACAAAGATCAAAATAAAAAATGGCTATCTGATGAACTCGCTGGTAATGCAGACAGCTGGAGAGAGTTCAA
  GCCCGGACCTAGGATTCCTGTCATAAGCCGCTCAAGGAAGGAATCCTTTCAGGAAAGTGACGATGCCTAC
  AGGTGGCAGGAGGGGAGAGGCTGCAGAGCTGTGAGAAGGCTGTTTCAGAAAGACCTCAGCAGCCTAGAAG
  CCATGTCAGAAATGGAAGCAGGAAGTCCTGAAAACAAGAAACAGCGGTCCAGACCTCGTAAGCCACGGAG
  GACTAGAACGGAGGACAGTGAGCAGGATGGAGATCTGGACGGTCCTGTCATCGACGAGTCTGTCCTTTCA
  ACCAAGGAGCTGTTGGGCCTCCAGCAGGCTGAGGAACGGCTCAAGAGAGACTGCATCGACAGGCTGAAAA
  GGCGACCACGAAACTGCCCGACAGCAAAGTATACCTGCAAACTGTGCGATGCTTTGATTGACTCCATTCC
  GTTTGCTCATAAGCACATCAAGGAGAAGAGGCACAAGAAGAACCTTAAGGAAAAGCAGGAGGAAGAATTG
  CTCACCACACTGCCCCCGCCAGCGCCCTCCCAGATACATGCAGTTGGCAGCGCCATTGACAGAGTGGTGC
  AGGAGTTTGGTTTACACAGTGAAAACTTGGATCAGAGACTAGAAATTAAGCGTGTCATGGAAAGTGTGTT
  CCGGCACAAGCTACCAGACTGTTCTCTTAGGCTTTATGGATCCTCCTGTAGCCGACTGGGCTTCAGAGAC
  TCTGACGTGAACATTGATGTTCAGTTTCCAGCTGTCATGTCTCAGCCAGATGTCCTCTTACTTGTTCAAG
  AATGTTTAAAGAACAGTGACTCCTTTATTGATGTTGATGCTGATTTCCATGCCCGGGTCCCTGTGGTGGT
  GTGCAGAGATAAGCAAAGCGGCCTTCTTTGTAAAGTAAGTGCAGGAAACGAAAACGCTTGGCTGACCACA
  AAGCACCTAACAGCTCTTGGAAAACTAGAGCCAAGGCTGGTTCCTCTGGTGATTGCATTCAGATACTGGG
  CAAAGCTTTGTAGTATTGATCGCCCCGAAGAAGGAGGCCTGCCACCATATGTGTTTGCTCTGATGGCTGT
  TTTCTTCCTTCAGCAAAGAAAAGAACCTCTCTTGCCAGTTTATCTAGGATCATGGATTGAAGAATTCTCA
  TTAAATAAATTAGGGAATTTCAGCCTTAAAGATGTTGAGAAAGACTCTGTGGTCTGGGAATACACTGACA
  ACAGCACAGGGGACACCAGCTCAGCAAAAGAGGAGGCACCCAAAGAGACTGCAGCAAAGAAGGGACAGGT
  GCCATTGACATTTAATATAAAGCATCAGCCTTCAGTGCCAGTCGGGCAACTCTGGGTGGAATTGCTGCGG
  TTCTATGCTTTAGAGTTTAATCTGGCTGATTTAGTGATCAGTATTCGTGTCAAAGAATTAATATCACGGG
  AATCAAAGGACTGGCCCAAAAAGCGTATCGCAATTGAAGACCCCTATTCTGTTAAAAGAAATGTGGCAAG
  GACCCTGAATAACCAGCCTGTGTTTGAGTATATACTCCATTGTTTGAGGACAACATATAAATATTTCGCT
  CTTCCACACAAAGTTACAAAACCCAACCTCACAAAGCCTCCTAGCCCAGTCACCTGTGTTTCAGACCCTT
  ACAGAGAAGCAAAAAATGGTGGCCCAGAACCCCAAGCAACAAATATTGATAAACTGGGAAATGCAGCTGT
  GGCTCAAGATCCTGGTGTTCAAACCTCAGGTGACTGCAGAGCACAGCTAGTTACTCTTAAGAATACCACT
  GAGGAGGTGGGAAGCCCAGCAAAGGAGAAGACAGGAGGTGTGCACATCCCAGCCCACCAGGAGAGCTCTG
  GCTGTGTCCAGGCAGAGGTCAGTTGTGAGGGTTTGGAGGATGCCACAGCAGAGCTTCCGGAAACAGGAAG
  TGACAACGAGGAAGTCAGAAGGAAGACCAAGCATCCTTTGAGCACTGATGACCAAGGTTTAAGCAGCAGC
  AAGCACCCAGAACTGCAGAACTGTGGCAGCCTGTGTGGTCTGCAGGCAGACAACACTCTGGAGTTAGTAG
  CTGAAGAGTGTAATAGCTGTGCCTCTTTGGACAACAAGGCTGAGGTCAATGAAGAGAGGATAGAAGGCGC
  TGAGGAACTAGAAGAAGCAGCAGCTCTGAGTTGCTTTTCCCCTTCAGTACAGAGTCGCACATCTGCAGCC
  ATGCACTTTGATGATGAGGAGGAGGAGGAGGAAGAAGAGGAGGAAGAGGAGCCCAGGCTCAGCATTAACC
  TTACAGAAGATGAGGAGGGGGTTGCTAATGAGCATCAGGTGGACAGCAGGTATGCTGGGTCAGGTGAGGA
  GGATGCCCTCTCTGAAGAAGATGACTTGGCAGAGCCTGCTAAGGGTGAAGATACAGGAGAATGTGGAGAA
  AACGTGGGTGGGACTCTGCTCATAGACCTAAATAGAATAACACTTAAAGAAGAAAGCTTTCCTGAGGAAG
  ATTTACCTGGGGACCAGTCTGAGTTCTTCTACGAATTTAGAAAACTGACCTTCACCAAAGGCAAGTCCCC
  CACAGTAGTGTGCAGCTTGTGTAAGCGAGAAGGTCACCTAAAGAAGGACTGTCCTGAGGACTTCAAGAGG
  GTCCAGCTGGAGCCGTTGCCACCCCTGACACCCAAGTTCTCAAACATCTTAGATCAAGTCTGCGTCCAGT
  GTTACAAGGATTTTTCTCCAACTATTGTAGAAGATCAGGCTCGTGAACATATCCGGCAAAATCTAGAAAG
  TTTCATAAAACAGGATTTTCCAGGAACTAAATTGAGCTTGTTTGGCTCCTCCAAAAATGGATTTGGGTTC
  AAACAGAGTGACCTTGACGTCTGTATGACGATTAACGGACATGAGACTGCTGAGGGGCTGGACTGTGTAA
  GAACTATTGAGGAGCTGGCTCGAGTCCTCAGAAAGCACTCAGGGTTGAGGAATATCCTTCCTATCACAAC
  AGCCAAGGTGCCCATCGTGAAGTTCTTCCATCTAAGAAGTGGTCTGGAAGTGGACATCAGCTTGTATAAC
  ACACTGGCGCTTCATAACACAAGGCTCTTGTCTGCATACTCAGCCATTGACCCCAGAGTGAAGTATCTGT
  GCTACACCATGAAAGTGTTCACAAAGATGTGTGACATCGGTGACGCGTCCAGAGGCAGCTTGTCATCCTA
  CGCTTACACTCTTATGGTGCTATACTTCCTCCAGCAAAGGTCTCCACCTGTCATCCCCGTGCTTCAAGAG
  ATATACAAAGGTGAAAAGAAACCAGAAATACTTGTTGATGGCTGGAATATTTACTTTTTCGATCAAATAA
  ATGAACTGCCCACTTGTTGGCCAGAATATGGGAAAAATACAGAACCTGTCGGGCAGCTGTGGCTGGGACT
  TCTGCGCTTCTACACTGAGGAGTTTGACTTTAAGGAGCACGTGATCAGCATCAGAAGAAAGAGTCTGCTT
  ACAACTTTTAAGAAACAGTGGACCTCAAAATACATTGTTATTGAAGATCCCTTCGATTTGAATCACAATC
  TTGGTGCTGGATTATCGAGGAAAATGACAAATTTTATAATGAAAGCTTTTATCAATGGTAGAAGAGTATT
  TGGAATTCCAGTCAAAGGATTTCCAAAGGACAACCCCTCAAAACTGGCATACTTTTTTGATCCGGATGTC
  CTAACTGAGGGAGAACTGGCCCCAAATGACAGGTGCTGCCGAATTTGTGGGAAAATCGGGCACTTCATGA
  AGGACTGTCCCATGAGGAGGAAAGTGAGGCGACGGAGAGACCAGGAAGATACCCCAAACCAAAGGTACTC
  TGAGAGCAAAGAAAAAAGAAGCAAAGAGGACAAAGAGATTCAGAACAAGTACACAGAAAAGGAAGTGTCA
  ACAAAAGAAGATAAGCTCACACCGTGTGCAGCTGCGAAAGCCAAGCCAGTGAGGGCAGCTGTCGACCTGG
  GCCGGGAGAAGCTCCTCAGGACGCCCACAGAAAAGTGGAAGAGACAGGACGACAGAGACTCGAGAGAAAA
  GCGTTGTTTCATCTGTGGAAGAGAAGGACACATTAAAAAGGAATGCCCACAGTTCAAAGGCTCTCCAGGC
  AGTGCTTTTACATGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_153538
• Insert Size: 4425 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153538.3, NP_705766.3
• RefSeq Size: 5569 bp
• RefSeq ORF: 4425 bp
• Locus ID: 214290
• UniProt ID: Q5BLK4
• Cytogenetics: 13 B2
• Gene Summary: Uridylyltransferase that mediates the terminal uridylation of mRNAs with short (less than 25 nucleotides) poly(A) tails, hence facilitating global mRNA decay (PubMed:28792939). Essential for both oocyte maturation and fertility. Through 3' terminal uridylation of mRNA, sculpts, with TUT7, the maternal transcriptome by eliminating transcripts during oocyte growth (PubMed:28792939). Involved in microRNA (miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets. Also acts as a suppressor of miRNA biogenesis by mediating the terminal uridylation of miRNA precursors, including that of let-7 (pre-let-7) (PubMed:22898984). Uridylated pre-let-7 RNA is not processed by Dicer and undergo degradation. Pre-let-7 uridylation is strongly enhanced in the presence of LIN28A. Due to functional redundancy between ZCCHC6 and ZCCHC11, the identification of the specific role of each of these proteins is difficult (By similarity) (PubMed:22898984). Involved in microRNA (miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets (By similarity). Also functions as an integral regulator of microRNA biogenesiS using 3 different uridylation mechanisms (By similarity). Acts as a suppressor of miRNA biogenesis by mediating the terminal uridylation of some miRNA precursors, including that of let-7 (pre-let-7). Uridylated pre-let-7 RNA is not processed by Dicer and undergo degradation. Pre-let-7 oligouridylation is strongly enhanced in the presence of LIN28A (PubMed:22898984). In the absence of LIN28A, TUT7 and TUT4 monouridylate group II pre-miRNAs, which includes most of pre-let7 members, that shapes an optimal 3' end overhang for efficient processing (By similarity). Add oligo-U tails to truncated pre-miRNAS with a 5' overhang which may promote rapid degradation of non-functional pre-miRNA species (By similarity). Does not play a role in replication-dependent histone mRNA degradation (By similarity). Due to functional redundancy between TUT4 and TUT7, the identification of the specific role of each of these proteins is difficult (PubMed:28792939, PubMed:22898984). TUT4 and TUT7 restrict retrotransposition of long interspersed element-1 (LINE-1) in cooperation with MOV10 counteracting the RNA chaperonne activity of L1RE1. TUT7 uridylates LINE-1 mRNAs in the cytoplasm which inhibits initiation of reverse transcription once in the nucleus, whereas uridylation by TUT4 destabilizes mRNAs in cytoplasmic ribonucleoprotein granules (By similarity).[UniProtKB/Swiss-Prot Function]