Mug2 (NM_008646) Mouse Untagged Clone

CAT#: MC224480

Mug2 (untagged) - Mouse murinoglobulin 2 (Mug2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mug2
• Synonyms: Cpamd8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224480 representing NM_008646
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGAAGAGCAGACGGGCTCAGCTGTGTCTTTTTTCAGTTCTTCTTGCCTTCCTGCCTTCTGCTTCTT
  CACTCAATGGAGACTCGAAGTATATGGTGCTGGTCCCTTCTCAGCTCTACACTGAGACCCCTGAGAAAAT
  CTGCCTCCATTTATACCATCTGAATGAGACAGTGACCGTTACAGCTTCCTTGGTATCTCAAACGGGAAGG
  AGAAACCTGTTCGATGAGCTTGTGGTTGACAAGGACTTGTTCCAATGTGTTTCCTTCATTATCCCTACAC
  TCAACTCTCCTGATGAGGAGGAGTTTCTCTACGTGGACATCAAAGGGCCAACCCACGAATTCAGCAAAAG
  GAATGCAGTGCTTGTGAAGAACAAAGAAAGTGTTGTCTTTGTCCAGACAGATAAGCCCGTGTACAAGCCA
  GGACAATCAGTTAAATTTCGGGTTGTCTCTATGGATAAAACTCTACGCCCCCTGAATGAGTTGCTTCCTC
  TGGCTTACATTGAGGATCCGAAAAAGAACCGAATTATGCAGTGGAGGGATATTAAGACTGAGAATGGGCT
  TAAGCAAATGTCCTTCAGCCTGGCAGCAGAGCCCATTCAGGGCCCCTACAAGATAGTGGTGCACAAACAG
  TCAGGGGTGAAGGAAGAACACTCCTTTACTGTGATGGAATTTGTGCTTCCCAGATTTAATGTCGACCTGA
  AGGTCCCAAATGCCATCTCTGTGAATGATGAAGTGCTCCAAGTGACTGTATGTGGGAAATATACCTATGG
  GAAGCCTGTTCCAGGACAGGTGAAGATAAGTATCTGCCATGAGACTGAAGCTGGATGCAAAGAAGTCAAT
  TCCAAGCTAGACAACAATGGCTGCAGTACACAAGAAGTGAACATCACTGAGCTCCAATCAAAGAAAAGGA
  ATTATGAGGTTCAGCTTTTCCATGTGAATGCAACTGTTACAGAAGAAGGGACAGGACTGGAGTTCAATGG
  ATATGGAACAACTAAAATTGAAAGAATCACAAACAAGCTCATATTTCTGAAGGCAGATTCACACTTCAGA
  CATGGGATTCCATTCTTTGTGAAAGTCCGCCTAGTGGATATCAAGGGAGACCCTATCCCAAATGAGAGAG
  TCTTCATCAAAGCACAAGTACTTGGCTATACCAGTGCTACTACCACTGATCAGCATGGCCTGGCAAAGTT
  CTCCATAGACACTGCTGGCTTCTCGGGCTCTTCCCTCCATATCAAAGTCAACCACAAAGGGAAAGATTCA
  TGTTACTTTTTCTATTGCATGGAGGAAAGATATGCAAGTGCAGAGCATGTGGCCTATGCTGTTTATTCCC
  TCAGCAAGAGCTACATCTACCTTGTCAAAGAGACCAGCAGCATCTTGCCCTGCAACCAGATTCACACAGT
  TCAGGCACATTTTATTCTGAAGGGGGACTTAGGAGTGCTGAAAGAGCTCGTTTTCTACTACCTGGTCATG
  GCCCAGGGAAGCATCATCCAGACTGGAAACCATACTCACCAGGTGGAGCCAGGAGAAGCTCCAGTAAAAG
  GCAACTTTGACTTGGAGATTCCTGTGGAGTTCAGCATGGCCCCCATGGCTAAAATGCTCATCTACACCAT
  CTTGCCTGATGGAGAAGTGATTGCAGATTCTGTAAACTTTGAAATTGAAAAGTGTCTTCGCAACAAAGTG
  GACCTGAGCTTCAGCTCATCTCAAAGTCTTCCCGCCTCACAAACCCGTCTGCAGGTCACAGCTTCTCCTC
  AATCCCTCTGTGGCCTGAGAGCTGTGGACCAGAGCGTGCTGCTCCTGAAGCCCGAGGATGAGCTCTCCCC
  TTCCTGGATATACAATCTGCCAGGTATGCAGCACAACAAATTCATTCCAAGTTCCAGTCTGTCTGAAGAC
  CGAGAAGACTGTATACTGTACAGTTCATGGGTGGCTGAGAAGCACACAGACTGGGTACCACATGGAAGAG
  AGAAGGATGTCTACAGATATGTGGAGGACATGGATTTAAAGGCATTCACCAACTTGAAGATCAAACTTCC
  TAAAATTTGTTTTGACTCTGCTCCAATGTCAGGGCCTCGTGGGAAATTTGATCTTGCATTTAGTAGTGAA
  GTTTCTGGGACATTGCAAAAGGGATCAAGTAAAAGACCTCAACCTGAAGAGCCACCACGTGAAGACCCAC
  CACCTAAAGATCCACTTGCCGAGACCATTCGAAAATATTTTCCTGAAACCTGGGTCTGGGATATAGTCAC
  AGTAAACTCCACAGGAGTGGCTGAAGTGGAAATGACAGTCCCTGACACCATCACTGAATGGAAGGCCGGA
  GCCCTCTGCCTATCCAATGACACTGGCCTTGGCCTCTCTTCTGTGGTACCTCTCCAAGCCTTCCAGCCCT
  TCTTTGTGGAAGTCTCATTGCCCTACTCCGTGGTCCGTGGAGAAGCCTTCATGCTCAAAGCCACTGTGAT
  GAACTACCTCCCTACAAGCATGCGGATGAGTGTGCAGCTGGAAGCCTCTCCTGATTTCACAGCTGTCCCA
  GTGGGAGATGACCACGATTCTTACTGCCTCAGTGCCAATGGGAGGCACACCTCATCCTGGCTGGTAACTC
  CCAAGTCTTTAGGGAATGTGAATTTCTCTGTGTCTGTGGAAGCACAACAGTCCTCAGAGCCCTGTGGTTC
  TGAGGTGGCCACAGTTCCTGAAACTGGGAGAAAGGACACAGTAGTCAAAGTCCTGATAGTTGAGCCTGAA
  GGAATCAAGCAAGAGCATACCTTCAACTCACTGTTCTGTGCATCAGATGCTGAGATATCTGAAAAAATGT
  CCCTGGTCCTCCCACCAACAGTAGTGAAAGATTCAGCAAGAGCGCATTTCTCTGTGATGGGTGATATCTT
  GAGTTCAGCCATAAAAAACACACAAAACCTTCTCCATATGCCCTATGGCTGTGGGGAGCAGAACATGGTC
  CTTTTTGCTCCCAACATCTACGTATTGAAATATCTGGATAAAACCCAACAGCTGACTCAGAAGATCAAGA
  CTAAGGCCCTCGGCTTCCTTAGAGCTGGTTATCAGAGAGAGCTGAACTACAAACACAAGGATGGCTCCTA
  CAGTGCCTTCGGGGATCAAAATGGCGAGAGAGAGGGAAACACTTGGCTCACAGCCTTTGTGCTCAAGTCT
  TTTGCCCAAGCTCGAGCCTTCATCTTCATCGATGAATCACACATCACCCATGCCTTCACCTGGCTCTCCC
  AGCAGCAGAAGGACAATGGCTGCTTCCGGAGCTCCGGATCATTGTTCCACAATGACATAAAGCATCCTGT
  TGTCTCCAAAGCCCTGAGCTGCCTGGAGTCTTCCTGGAAGACCATAGAACAAGGGCGAAATGCCAACTTT
  GTGTATACCAAGGCACTGATGGCCTATGCTTTCGCTCTGGCGGGAAACCAGGATAAGAGAAATGAAATCC
  TGAAGTCACTTGATGAGGAAGCTATAAAAGAAGACAACTCCATCCACTGGGAAAGACCTCAGAAACCCAG
  GAAATCCGAACACAATTTGTACAAACCCCAGGCTTCCTCTGTTGAAGTAGAGATGAATGCCTATGTGGTC
  CTGGCTCGCCTCACTGCCCAGCCAGCCCCATCCCCTGAGGATCTGACTTTGTCAAGGAGCACCATCATGT
  GGCTCACAAAGCAACAGAATTCCAATGGTGGCTTCTCCTCCACACAGGACACTGTGGTGGCCCTTGATGC
  TCTGTCCAAATATGGAGCAGTGACTTTTTCCAGAAGGCAGAAAACTTCTTTGGTGACCATCCAATCTACA
  GGGTCATTTTCCCAAAAGTTCCAAGTGGAGAACAGTAATTGCCTGTTACTTCAGCAGGTCCCATTACCAG
  ACATTCCTGGGGACTATACCATCAGTGTATCAGGGGAAGGATGTGTGTATGCTCAGACTACACTGAGATA
  CAACATGCACTTGGAGAAGCAGCAGTCTGCATTTGCTCTACGGGTACAGACAGTACCTCTAACTTGTAAT
  AACCCCAAAGGCCACAACAGCTTCCAGATCTCACTAGAAATCAGTTACACAGGCAGCCGGCCAGCCTCCA
  ACATGGTGATTGCTGATGTGAAGATGCTATCTGGTTTCATCCCATTGAAACCAACAGTGAAAAAGCTTGA
  AAGATTAGAGCACATAAGCAGAACAGAAGTGAGCAACAACAATGTCTTATTATATTTGGATCAGGTGACC
  AATCAGACACTGGCCTTCTCCTTCATCATTCAACAAGACATCTCAGTACGGAACCTGCAGCCTGCCATTG
  TGAAAGTCTATGACTACTATGAGACAGATGAAGTGGCTTATGCTGAATACAGCAGTCCCTGCAGCTCAGA
  CAAACAAAATGTTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008646
• Insert Size: 4356 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008646.3, NP_032672.2
• RefSeq Size: 4544 bp
• RefSeq ORF: 4356 bp
• Locus ID: 17837
• UniProt ID: P28666
• Cytogenetics: 6 F1
• Gene Summary: A proteinase activates the inhibitor by specific proteolysis in the bait region, which, by an unknown mechanism leads to reaction at the cysteinyl-glutamyl internal thiol ester site and to a conformational change, whereby the proteinase is trapped and/or covalently bound to the inhibitor. While in the tetrameric proteinase inhibitors steric inhibition is sufficiently strong, monomeric forms need a covalent linkage between the activated glutamyl residue of the original thiol ester and a terminal amino group of a lysine or another nucleophilic group on the proteinase, for inhibition to be effective.[UniProtKB/Swiss-Prot Function]