Dip2b (NM_172819) Mouse Untagged Clone

CAT#: MC224292

Dip2b (untagged) - Mouse DIP2 disco-interacting protein 2 homolog B (Drosophila) (Dip2b), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dip2b
• Synonyms: 4932422C22; AI317237; AI854602; mKIAA1463
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224292 representing NM_172819
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTGGCTTTCACAGAAGGTGCTGTGAACTGACTGTGGAAGAAAGGAGCTCTACAAGAGAAGAGGGAG
  CCTGGGTAGCCTTTCCATGTGTTCCTGTCAATAGCAGGGTGTCCACAAAAATTCAGCAGCTTCTCAACAC
  CCTGAAGCGACCCAAAAGACCCCCCTTAAAGGAATTTTTTGTGGATGACTCTGAAGAAATTGTGGAAGTA
  CCTCAGCCAGATCCCAACCAGCCAAAGCCTGAAGGCCGCCAGATGACCCCAGTGAAAGGAGAGCCCCTGG
  GCGTCATCTGTAACTGGCCACCTGCACTGGAATCTGCCCTGCAGCGCTGGGGCTCTACCCAAGCCAAGTG
  CCCCTGCCTGACTGGGCTGGATGTGACGGGGAAACCAGTCTACACTCTCACATACGGAAAGTTGTGGAGC
  AGAAGTTTAAAGTTGGCTTACACACTGCTTAATAAACTGGGAACAAAAAATGAACCTGTGTTAAAACCTG
  GAGACAGGGTTGCCCTGGTTTACCCCAACAATGACCCTGTCATGTTTATGGTGGCTTTTTATGGATGTCT
  CCTGGCAGAAGTGATTCCAGTACCCATAGAAGTACCCCTGACCAGAAAGGATGCCGGAGGTCAGCAGATT
  GGCTTCTTGCTAGGGAGCTGTGGGATTGCCTTAGCTCTCACCAGTGAAATTTGTCTGAAAGGCCTGCCAA
  AAACCCAGAATGGAGAAATTGTACAGTTTAAAGGTTGGCCTCGCCTCAAGTGGGTTGTGACAGATTCTAA
  GTACCTCTCGAAGCCGCCCAAAGACTGGCAGCCACACATATCACCTGCGGGCACGGAACCGGCATACATT
  GAGTATAAAACAAGCAAAGAAGGGAGTGTGATGGGAGTCACGGTGTCGCGGCTCGCAATGCTGTCTCAGT
  GCCAAGCTCTGTCACAGGCCTGCAATTATTCTGAAGGAGAAACTGTAGTAAATGTTTTAGACTTTAAGAA
  GGACGCTGGGCTGTGGCATGGCATGTTTGCGAATGTAATGAATAAGATGCATACAATTAGTGTACCCTAC
  TCTGTTATGAAGACCTGTCCTCTCTCTTGGGTCCAGAGAGTCCATGCTCACAAAGCCAAAGTAGCATTAG
  TGAAGTGTCGAGACTTACACTGGGCCATGATGGCACATCGGGACCAAAGGGACGTAAGCCTGAGCTCTCT
  GCGGATGCTGATTGTCACCGACGGCGCCAATCCCTGGTCTGTGTCGTCCTGTGATGCTTTCCTGAGTCTC
  TTCCAAAGCCACGGACTGAAACCTGAGGCCATCTGTCCATGTGCCACATCTGCAGAGGCCATGACTGTAG
  CCATCCGAAGGCCTGGGGTTCCAGGGGCTCCTTTGCCAGGAAGAGCCATTCTCTCAATGAATGGACTGAG
  CTATGGGGTCATACGGGTCAATACTGAGGATAAAAATTCAGCACTGACAGTTCAGGATGTGGGACATGTG
  ATGCCTGGAGGCATGATGTGCATTGTGAAACCAGACGGACTTCCTCAGCTCTGCAGGACAGATGAGATTG
  GAGAGATCTGTGTCAGCTCCAGGACTGGAGGGATGATGTACTTTGGACTTGCTGGTGTGACCAAAAATAC
  ATTTGAGGTGATTCCAGTGACTTCTTCAGGCTCTCCTGTAGGGGACGTACCCTTCATCCGGTCAGGGCTG
  CTGGGATTTGTAGGGCCTGGTAGCTTGGTGTTCGTGGTTGGAAAAATGGATGGATTACTCATGGTCAGCG
  GCCGAAGACATAACGCCGATGACATTGTTGCTACTGGATTGGCTGTGGAGTCCATAAAGACCGTCTATCG
  AGGAAGAATTGCTGTGTTTTCTGTGTCTGTGTTTTATGACGAGCGCATTGTGGTGGTGGCAGAACAAAGG
  CCCGATGCGTCTGAGGAAGACAGTTTCCAGTGGATGAGCCGGGTGCTGCAGGCAATCGACAGCATCCATC
  AAGTCGGCGTGTACTGTCTTGCCCTGGTGCCAGCTAACACGTTACCAAAAACCCCACTAGGAGGAATCCA
  TATATCCCAGACAAAACAGCTCTTTCTGGAGGGTTCTCTACACCCGTGTAATATCCTCATGTGCCCACAT
  ACGTGTGTGACGAACTTGCCAAAGCCCCGACAAAAGCAACCAGGTGTAGGCCCTGCTTCTGTGATGGTTG
  GGAATCTGGTTGCTGGTAAACGCATAGCACAGGCTGCCGGAAGGGACCTGGGCCAAATAGAAGAGAATGA
  CCTGGTGAGGAAGCACCAGTTCCTGGCAGAGATTTTGCAGTGGCGAGCCCAGGCAACTCCTGACCATGTC
  CTCTTCATGCTGTTAAATGCCAAGGGAACTACTGTGTGCACAGCCAGCTGCCTTCAGCTTCATAAGCGAG
  CAGAGAGGATCGCTTCAGTACTTGGTGACAAGGGACATCTGAATGCAGGGGACAATGTAGTATTGCTCTA
  CCCGCCTGGCATCGAGTTGATTGCTGCATTCTACGGCTGCCTGTATGCAGGATGTATACCTGTGACCGTG
  CGGCCCCCTCATGCGCAGAACCTCACTGCTACCTTGCCCACAGTACGCATGGTCGTTGATGTCAGCAAGG
  CAGCGTGTGTCCTTACCACTCAAACTCTGATGAGGTTGCTCAAGTCTCGAGAGGCAGCGGCAGCTGTGGA
  TGTGAAAACCTGGCCAGCCATCATTGATACAGATGACTTACCCAGGAAACGGCTACCTCAGCTGTATAAA
  CCGCCCACTCCTGAGATGTTGGCGTATCTTGATTTCAGCGTTTCCACAACTGGCATGCTCACAGGGGTGA
  AGATGTCCCACTCTGCAGTCAATGCTCTCTGCAGAGCCATCAAGCTGCAGTGCGAATTGTACTCTTCCCG
  GCAGATCGCCATCTGCCTTGACCCTTACTGTGGACTTGGCTTTGCACTCTGGTGTCTCTGCAGTGTGTAT
  TCGGGTCACCAGTCCGTCTTGATCCCTCCTATGGAGCTGGAAAACAACCTTTTCCTCTGGCTCGCCACTG
  TCAACCAGTACAAGATCAGGGACACCTTCTGCTCCTACTCAGTCATGGAGCTGTGCACTAAAGGGCTGGG
  GAACCAGGTGGAGGTGCTGAAGACCAGAGGGATCAATCTTTCTTGCATCCGGACCTGTGTGGTGGTGGCA
  GAGGAGCGGCCCCGTGTCAGCCTTCAGCAGTCCTTCTCCAAGCTCTTCAAAGACATCGGTCTGTCCCCTC
  GAGCTGTCAGCACCACTTTTGGATCCAGAGTCAATGTAGCAATATGTTTACAGGGAACCTCAGGGCCGGA
  TCCAACAACTGTGTATGTGGATCTGAAGTCGCTGAGACATGACAGGGTCCGTCTTGTGGAACGGGGTGCC
  CCCCAGAGCCTCCTGCTTTCTGAATCTGGAAAGATCTTACCTGGAGTAAAAGTAGTTATTGTCAATCCTG
  AGACCAAAGGACCTGTTGGAGACTCTCACCTGGGAGAGATCTGGGTGAATAGCCCCCATACCGCCAGTGG
  CTACTATACCATCTACGACAGCGAGACTCTTCAAGCTGATCATTTCAATACCCGTCTGAGCTTTGGAGAT
  GCTGCCCAGACACTCTGGGCCCGGACAGGATACCTCGGGTTTGTCCGCAGGACTGAGCTCACTGCAGCCA
  CTGGAGAACGCCATGACGCCTTGTATGTGGTGGGTGCGCTGGATGAGACACTGGAGCTGAGGGGATTGCG
  ATACCATCCTATCGATATAGAGACCTCCGTGTCCCGCGTCCACAGGAGCATTGCTGAATGTGCTGTGTTC
  ACGTGGACCAACTTGCTGGTGGTCGTTGTGGAACTATGCGGCTCTGAACAGGAAGCCCTGGACCTGGTTC
  CTCTCGTGACCAATGTGGTCCTGGAAGAGCATTACCTCATCGTGGGTGTTGTGGTTGTTGTGGACCCGGG
  CGTTGTCCCCATCAACTCCAGAGGAGAGAAGCAGAGGATGCACCTCCGAGACAGCTTCCTCGCCGACCAG
  TTAGACCCTATCTATGTGGCTTACAACATGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172819
• Insert Size: 4023 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172819.3, NP_766407.2
• RefSeq Size: 8127 bp
• RefSeq ORF: 4023 bp
• Locus ID: 239667
• Cytogenetics: 15 F1
• Gene Summary: Negatively regulates axonal outgrowth and is essential for normal synaptic transmission (PubMed:32153366). Not required for regulation of axon polarity (PubMed:32153366). Promotes acetylation of alpha-tubulin (PubMed:32153366).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences causes translation initiation at a downstream AUG and results in an isoform (2) with a shorter N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.