Abcb1a (NM_011076) Mouse Untagged Clone

CAT#: MC224126

Abcb1a (untagged) - Mouse ATP-binding cassette, sub-family B (MDR/TAP), member 1A (Abcb1a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Abcb1a
• Synonyms: Abcb4; Evi32; mdr-3; Mdr1a; Mdr3; P-gp; Pgp; Pgy-3; Pgy3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224126 representing NM_011076
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACTTGAAGAGGACCTTAAGGGAAGAGCAGACAAGAACTTCTCAAAGATGGGCAAAAAGAGTAAAA
  AGGAGAAGAAAGAAAAGAAACCAGCAGTCAGTGTGCTTACAATGTTTCGTTATGCAGGTTGGCTGGACAG
  GTTGTACATGCTGGTGGGAACTCTGGCTGCTATTATCCATGGAGTGGCGCTCCCACTTATGATGCTGATC
  TTTGGTGACATGACAGATAGCTTTGCAAGTGTAGGAAACGTCTCTAAAAACAGTACTAATATGAGTGAGG
  CCGATAAAAGAGCCATGTTTGCCAAACTGGAGGAAGAAATGACCACGTACGCCTACTATTACACCGGGAT
  TGGTGCTGGTGTGCTCATAGTTGCCTACATCCAGGTTTCATTTTGGTGCCTGGCAGCTGGAAGACAGATA
  CACAAGATCAGGCAGAAGTTTTTTCATGCTATAATGAATCAGGAGATAGGCTGGTTTGATGTGCATGACG
  TTGGGGAGCTCAACACCCGGCTCACAGATGATGTTTCCAAAATTAATGAAGGAATTGGTGACAAAATCGG
  AATGTTCTTCCAGGCAATGGCAACATTTTTTGGTGGTTTTATAATAGGATTTACCCGTGGCTGGAAGCTA
  ACCCTTGTGATTTTGGCCATCAGCCCTGTTCTTGGACTGTCAGCTGGTATTTGGGCAAAGATATTGTCTT
  CATTTACTGATAAGGAACTCCATGCTTATGCAAAAGCTGGAGCAGTTGCTGAAGAAGTCTTAGCAGCCAT
  CAGAACTGTGATTGCGTTTGGAGGACAAAAGAAGGAACTTGAAAGGTACAATAACAACTTGGAAGAAGCT
  AAAAGGCTGGGGATAAAGAAAGCTATCACGGCCAACATCTCCATGGGTGCAGCTTTTCTCCTTATCTATG
  CATCATATGCTCTGGCATTCTGGTATGGGACTTCCTTGGTCATCTCCAAAGAATACTCTATTGGACAAGT
  GCTCACTGTCTTCTTTTCCGTGTTAATTGGAGCATTCAGTGTTGGACAGGCATCTCCAAATATTGAAGCC
  TTCGCCAATGCACGAGGAGCAGCTTATGAAGTCTTCAAAATAATTGATAATAAGCCCAGTATAGACAGCT
  TCTCAAAGAGTGGGCACAAACCAGACAACATACAAGGAAATCTGGAATTTAAGAATATTCACTTCAGTTA
  CCCATCTCGAAAAGAAGTTCAGATCTTGAAGGGCCTCAATCTGAAGGTGAAGAGCGGACAGACGGTGGCC
  CTGGTTGGCAACAGTGGCTGTGGAAAAAGCACAACTGTCCAGCTGATGCAAAGGCTCTACGACCCCCTAG
  ATGGCATGGTCAGTATCGACGGACAGGACATCAGAACCATCAATGTGAGGTATCTGAGGGAGATCATTGG
  TGTGGTGAGTCAGGAACCTGTGCTGTTTGCCACCACGATCGCCGAGAACATTCGCTATGGCCGAGAAGAT
  GTCACCATGGATGAGATTGAGAAAGCTGTCAAGGAAGCCAATGCCTATGACTTCATCATGAAACTGCCCC
  ACCAATTTGACACCCTGGTTGGTGAGAGAGGGGCGCAGCTGAGTGGGGGACAGAAACAGAGAATCGCCAT
  TGCCCGGGCCCTGGTCCGCAATCCCAAGATCCTTTTGTTGGACGAGGCCACCTCAGCCCTGGATACAGAA
  AGTGAAGCTGTGGTTCAGGCCGCACTGGATAAGGCTAGAGAAGGCCGGACCACCATTGTGATAGCTCATC
  GCTTGTCTACCGTTCGTAATGCTGACGTCATTGCTGGTTTTGATGGTGGTGTCATTGTGGAGCAAGGAAA
  TCATGATGAGCTCATGAGAGAAAAGGGCATTTACTTCAAACTTGTCATGACACAGACAGCAGGAAATGAA
  ATTGAATTAGGAAATGAAGCTTGTAAATCTAAGGATGAAATTGATAATTTAGACATGTCTTCAAAAGATT
  CAGGATCCAGTCTAATAAGAAGAAGATCAACTCGCAAAAGCATCTGTGGACCACATGACCAAGACAGGAA
  GCTTAGTACCAAAGAGGCCCTGGATGAAGATGTACCTCCAGCTTCCTTTTGGCGGATCCTGAAGTTGAAT
  TCAACTGAATGGCCTTATTTTGTGGTTGGTATATTCTGTGCCATAATAAATGGAGGCTTACAGCCAGCAT
  TCTCCGTAATATTTTCAAAAGTTGTAGGGGTTTTTACAAATGGTGGCCCCCCTGAAACCCAGCGGCAGAA
  CAGCAACTTGTTTTCCTTGTTGTTTCTGATCCTTGGGATCATTTCTTTCATTACATTTTTTCTTCAGGGC
  TTCACATTTGGCAAAGCTGGAGAGATCCTCACCAAGCGACTCCGATACATGGTTTTCAAATCCATGCTGA
  GACAGGATGTGAGCTGGTTTGATGACCCTAAAAACACCACCGGAGCACTGACCACCAGGCTCGCCAACGA
  TGCTGCTCAAGTGAAAGGGGCTACAGGGTCTAGGCTTGCTGTGATTTTCCAGAACATAGCAAATCTTGGG
  ACAGGAATCATCATATCCCTAATCTATGGCTGGCAACTAACACTTTTACTCTTAGCAATTGTACCCATCA
  TTGCGATAGCAGGAGTGGTTGAAATGAAAATGTTGTCTGGACAAGCACTGAAAGATAAGAAGGAACTAGA
  AGGTTCTGGAAAGATTGCTACGGAAGCAATTGAAAACTTCCGCACTGTTGTCTCTTTGACTCGGGAGCAG
  AAGTTTGAAACCATGTATGCCCAGAGCTTGCAGATACCATACAGAAATGCGATGAAGAAAGCACACGTGT
  TTGGGATCACGTTCTCCTTCACCCAGGCCATGATGTATTTTTCTTATGCTGCTTGTTTCCGGTTCGGTGC
  CTACTTGGTGACACAACAACTCATGACTTTTGAAAATGTTCTGTTAGTATTCTCAGCTATTGTCTTTGGT
  GCCATGGCAGTGGGGCAGGTCAGTTCATTCGCTCCTGACTATGCGAAAGCCACAGTGTCAGCATCCCACA
  TCATCAGGATCATTGAGAAAACCCCCGAGATTGACAGCTACAGCACGCAAGGCCTAAAGCCGAATATGTT
  GGAAGGAAATGTGCAATTTAGTGGAGTCGTGTTCAACTATCCCACCCGACCCAGCATCCCAGTGCTTCAG
  GGGCTGAGCCTTGAGGTGAAGAAGGGCCAGACGCTGGCCCTGGTGGGCAGCAGTGGCTGCGGGAAGAGCA
  CAGTGGTCCAGCTGCTCGAGCGCTTCTACGACCCCATGGCTGGATCAGTGTTTCTAGATGGCAAAGAAAT
  AAAGCAACTGAATGTCCAGTGGCTCCGAGCACAGCTGGGCATTGTGTCCCAAGAGCCCATTCTCTTTGAC
  TGCAGCATCGCAGAGAACATTGCCTACGGAGACAACAGCCGGGTCGTGTCTTATGAGGAGATTGTGAGGG
  CAGCCAAGGAGGCCAACATCCACCAGTTCATCGACTCGCTACCTGATAAATACAACACCAGAGTAGGAGA
  CAAAGGCACTCAGCTGTCGGGTGGGCAGAAGCAGCGCATCGCCATCGCACGCGCCCTCGTCAGACAGCCT
  CACATTTTACTTCTGGACGAAGCAACATCAGCTCTGGATACAGAAAGTGAAAAGGTTGTCCAGGAAGCGC
  TGGACAAAGCCAGGGAAGGCCGCACCTGCATTGTGATCGCTCACCGCCTGTCCACCATCCAGAACGCGGA
  CTTGATCGTGGTGATTCAGAACGGCAAGGTCAAGGAGCACGGCACCCACCAGCAGCTGCTGGCGCAGAAG
  GGCATCTACTTCTCAATGGTCAGTGTGCAGGCTGGAGCAAAGCGCTCATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011076
• Insert Size: 3831 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011076.2, NP_035206.2
• RefSeq Size: 4977 bp
• RefSeq ORF: 3831 bp
• Locus ID: 18671
• UniProt ID: P21447
• Cytogenetics: 5 3.43 cM
• Gene Summary: The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. This gene encodes a p-glycoprotein which actively transports a variety of hydrophobic amphipathic drugs and plays a major role in the blood-brain barrier permeability of certain drugs. [provided by RefSeq, Jul 2008]