Plch2 (NM_175556) Mouse Untagged Clone

CAT#: MC224044

Plch2 (untagged) - Mouse phospholipase C, eta 2 (Plch2), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Plch2
• Synonyms: A930027K05Rik; Plc-eta2; PLCeta2; Plcl4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224044 representing NM_175556
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGGACTGGCCTGGGGACCATCCAGGGCCGCAGGCAGCAGTTGGGTCAATGCCAGTGGGACCTGGG
  AACAGCCCTTGCGGGGCTTCAGTGGGCTCCAAGGAGGAAGACGAAGAGGTAGAGGAGAGAAGGGGATCCC
  TGAGGAGCCCCTGTGCCAGCTGACACCTCAGCTTGGCCTGTCGCTGAGGGTCCCTTTTGGCTTGGGGGAC
  TACGGCTTGGATATGCCTGGTCCCCAGCCGTCTGCTGCCAGCCAGACCACAGGAGCCGTGGCTTGCCTGG
  CAGAGGTACTCCTCTGGGTTGGAGGGAGCGTGGTAGTGTCACCAAGATGGCAGCTCAGCCTTGTAGTGGA
  GCGATGCATGAGTGCCATGCAAGAGGGGACCCAGATGGTGAAGCTCCGTGGCAGCTCCAAGGGATTGGTC
  CGCTTCTACTACCTGGATGAGCACCGCTCCTGCCTCCGATGGCGTCCCTCCCGCAAGAACGAGAAAGCCA
  AGATTTCCATCGACTCCATCCAGGAGGTGAGTGAGGGCCGGCAGTCTGAAATCTTCCAGCGCTACCCGGA
  CAGCAGCTTTGACCCCAACTGCTGCTTCAGCATCTACCATGGCAGCCACAGAGAGTCGCTGGACTTGGTC
  TCACCCAGCAGTGAAGAGGCACGTACCTGGGTCACCGGCCTTCGCTACCTCATGGCTGGCATCAGCGATG
  AAGACAGCCTAGCCCGTCGCCAGCGTACCAGGGACCAGTGGCTGAAGCAGACGTTTGATGAGGCTGACAA
  GAACGGGGACGGCAGCCTGAGCATCAGTGAGGTTCTGCAGCTGCTACATAAACTCAACGTGAACCTGCCC
  CGGCAGAGGGTGAAACAGATGTTCAGGGAGGCGGACACAGATGACCATCAGGGGACATTAGGCTTTGAGG
  AATTCTGCGCCTTCTACAAGATGATGTCTACCCGCCGAGACCTCTACCTGCTCATGCTGACCTACAGCAA
  CCATAAGGACCACTTGGATGCCTCCGACCTGCAGCGCTTTCTGGAGGTGGAGCAGAAGATGAACGGTGTG
  ACCCTGGAGAGCTGTCAGAACATCATTGAGCAGTTTGAGCCTTGCCTGGAAAATAAGAGCAAGGGGATGC
  TGGGGATTGATGGCTTTACCAACTACACCCGGAGCCCCGCCGGTGACATCTTCAACCCTGAGCACAACAG
  AGTGCACCAAGACATGACGCAGCCGCTGAGTCACTACTTCATCACCTCGTCCCACAACACCTACCTCGTG
  GGTGACCAGCTCATGTCCCAGTCTCGGGTGGACATGTATGCCTGGGTCCTGCAGGCCGGCTGCCGCTGTG
  TGGAGGTGGACTGCTGGGACGGGCCCGATGGAGAACCCATTGTCCATCATGGCTACACTCTGACCTCCAA
  GATCCTTTTCAAAGATGTCATCGAAACCATCAACAAATACGCCTTCATCAAGAATGAGTACCCAGTGATC
  CTGTCCATTGAGAACCACTGTAGTGTTGTTCAGCAGAAGAAGATGGCCCAGTATCTGACTGACATCCTCG
  GGGACAAGCTGGACCTCTCCTCAGTGAGCAGCGAAGATGCCACCATGCTTCCATCTCCACAGATGCTCAA
  GGGCAAGATCCTGGTGAAGGGGAAGAAGCTCCCTGCCAATATCAGTGAGGATGCAGAAGAAGGCGAAGTA
  TCTGATGAGGACAGTGCTGATGAGATGGAGGATGACTGCAAGCTCCTCAATGGGGATGCCTCCACCAATC
  GGAAGCGTGTGGAAAACATTGCAAAGAAGAAGCTGGATTCTCTGATCAAGGAGTCAAAGATCCGTGACTG
  TGAAGACCCCAATGACTTCTCTGTGTCCACACTATCCCCCTCTGGAAAGCTTGGGCGCAAAGCAGAGGCC
  AAGAAGGGTCAGAGCAAGGTTGAGGAAGACGTGGAGGCCGGGGAAGACAGCGGGGTGAGCAGGCAGAATA
  GCCGCCTCTTCATGAGCAGCTTCTCCAAGCGCAAGAAAAAAGGCAGCAAGATAAAGAAGGTGGCCAGCGT
  GGAAGAGGGGGACGAGACCCTGGACTCCCCAGGAAGCCAGAGCCGAGGGACTGCCCGGCAAAAGAAGACC
  ATGAAGCTGTCACGAGCCCTCTCGGACCTAGTGAAATATACCAAGTCTGTGGGGACCCATGACGTGGAGA
  TAGAGGTGGTATCTAGCTGGCAGGTGTCGTCCTTCAGTGAGACCAAGGCCCATCAGATCCTGCAGCAGAA
  GCCCACCCAGTACCTGCGCTTCAACCAGCACCAGCTCTCGCGCATATACCCCTCCTCCTACCGTGTGGAC
  TCCAGCAACTACAATCCACAACCCTTCTGGAACGCTGGTTGCCAGATGGTTGCCCTGAACTACCAGTCAG
  AGGGGCGGATGCTACAGCTGAACAGGGCCAAGTTCAGCGCCAACGGTGACTGTGGCTACGTGCTCAAACC
  CCAGTGCATGTGCCAGGGTGTCTTCAACCCCAACTCGGAGGATCCCCTGCCGGGGCAGCTCAAGAAGCAG
  CTGGCCCTGAGGATCATCAGTGGCCAGCAGCTGCCCAAACCACGGGACTCGGTGCTGGGCGACCGTGGGG
  AGATCATCGACCCCTTCGTGGAGGTGGAGGTCATTGGGCTCCCCGTGGACTGCAGCAAGGAGCAGACCCG
  AGTGGTGGACGACAACGGATTCAACCCCATGTGGGAGGAGACACTGGTGTTCACCGTGCACATGCCAGAG
  ATTGCGCTTGTACGCTTCCTGGTCTGGGACCATGACCCCATTGGACGTGACTTCATCGGCCAGAGGACAC
  TAGCCTTCAGCAGCATAATGCCAGGCTACCGGCATGTGTACCTAGAAGGGATGGAAGAGGCCTCTATCTT
  TGTTCATGTGGCTGTCAGTGACATTAGTGGTAAGGTCAAGCAGACTCTGGGTCTAAAAGGTCTCTTCCTC
  CGAGGCACAAAGCCAGGCTCGCTGGACAGTCATGCTGCTGGACAGCCCCTCCCCCGGCCCTCCGTTAGCC
  AGAGGCTCCTGCGGCGCACGGCCAGTGCCCCGACCAAAAGCCAGAAGCCAAGTCGCAAGGGCTTCCCAGA
  GCTGGCCCTGGGCACACAGGACGCAGGCTCCGAGGGGGCAGCTGATGACGTGGCACCCTCTAGCCCCAAC
  CCAGCTCTGGAGGCCCCTACTCAAGAGAGGTCAGGCAGCAGCAGCCCCCGAGGTAAGGCACCAGGAGGAG
  AAGCAACAGAAGAGAGGACACTGGCACAGGTGCGGTCCCCAAATGCCCCCGAAGGCCCTGGACCTGCAGG
  GATGGCCGCCACATGCATGAAGTGTGTGGTGGGCTCCTGCGCCGGTATGGACGTTGAGGGCCTTCAAAGG
  GAGCAACAACCCAGCCCTGGGCCTGCAGGCAGCCACATGGCCATCAGCCATCAGCCCAGGGCCCGGGTAG
  ATTCACTGGGGGGCCCTTGCTGCAGCCCAAGTCCTCGAGCCACCCCAGGGAGAAGCAAAGAGGCCCCCAA
  GGGTCCTAGGGCTCGGCGCCAGGGTCCAGGCGGCGGCTCTGTGTCCTCGGACTCCAGCAGCCCAGACAGC
  CCAGGCAGCCCCAAGGTGGCCCCCTGCCAGCCGGAGGGTGCTCACAGGCAGCAGGGGGCGCTGCAGGGAG
  AGATGAACGCCTTGTTCGTTCAAAAGCTGGAGGAGATCAGGAGTCATTCCCCCATGTTCTCCACCGTTAG
  GGATTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_175556
• Insert Size: 3717 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175556.4, NP_780765.2
• RefSeq Size: 5598 bp
• RefSeq ORF: 3717 bp
• Locus ID: 269615
• UniProt ID: A2AP18
• Cytogenetics: 4 E2
• Gene Summary: The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. This phospholipase activity is very sensitive to calcium. May be important for formation and maintenance of the neuronal network in the postnatal brain.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) uses an alternate splice site and contains an alternate exon in the 3' coding region, resulting in an early stop codon, compared to variant 1. It encodes isoform a, which is shorter and has a distinct C-terminus, compared to isoform b.