Mical2 (NM_001193305) Mouse Untagged Clone

CAT#: MC223581

Mical2 (untagged) - Mouse microtubule associated monoxygenase, calponin and LIM domain containing 2 (Mical2), transcript variant A, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mical2
• Synonyms: 5330438E18Rik; 9530064J02; MICAL-2; mKIAA0750
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223581 representing NM_001193305
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGAGAATGAAGATGAGAAGCAGGCGCAGGCCAGCCAGGTCTTCGAGAACTTTGTGCAAGCTACCA
  CATGCAAAGGGACCCTCCAGGCCTTCAACATCCTCACCTGCCTCCTGGACCTAGATCCGCTGGACCATAG
  GAACTTCTACTCCCAGCTCAAGTCCAAGGTGAACACCTGGAAGGCCAAAGCCCTGTGGCACAAACTGGAT
  AAGCGCGGCTCCCACAAGGAGTACAAGCGAGGAAAAGCCTGCTCGAACACTAAGTGTCTCATCGTCGGAG
  GAGGACCATGTGGCTTGCGCACTGCCATTGAACTTGCCTACCTGGGAGCCAAAGTGGTTGTGGTGGAGAA
  GAGGGACACCTTCTCCCGGAACAATGTCCTGCACCTCTGGCCCTTCACTATCCATGACCTGCGGGGCCTG
  GGGGCCAAGAAGTTCTATGGGAAATTCTGTGCTGGCTCCATCGACCACATCAGTATCCGACAACTGCAGC
  TTATCCTCTTCAAGGTGGCCCTGATGCTGGGAGTGGAGGTCCACGTGAATGTGGAGTTTGTGAGGGTGCT
  GGAGCCTCCTGAAGACCAAGAGAATCAAAAAGTTGGATGGCGGGCAGAATTCCTTCCTGCAGACCACGCC
  CTGTCTGACTTTGAGTTTGATGTCATCATCGGTGCTGACGGTCACAGGAACACGCTAGAAGGCTTCAGGA
  GGAAAGAGTTCCGAGGGAAGCTGGCCATCGCCATCACCGCCAACTTCATAAACAGGAACAGCACAGCTGA
  GGCCAAGGTGGAGGAGATCAGTGGTGTTGCCTTCATCTTCAACCAGAAGTTCTTCCAGGACCTGAAGGAA
  GAAACAGGGATTGATCTCGAGAACATTGTTTACTATAAGGACAGTACCCACTACTTTGTCATGACAGCCA
  AGAAGCAGAGCCTGCTGGACAAGGGCGTCATCCTTAATGACTACATTGACACAGAGATGCTGCTGTGTTC
  GGAGAATGTGAACCAGGACAACCTGCTCTCCTACGCCAGAGAAGCCGCTGACTTTGCCACCAACTACCAG
  CTGCCATCCTTAGACTTTGCCATCAATCACAACGGGCAGCCTGACGTGGCCATGTTCGACTTCACCTCCA
  TGTATGCCTCAGAGAACGCAGCTCTGATGCGTGAGCGCCAGGCACACCAGCTGCTCGTGGCTCTTGTGGG
  CGACAGCCTGCTTGAGCCATTTTGGCCCATGGGCACAGGCTGTGCCCGAGGCTTCCTGGCAGCCTTTGAC
  ACGGCATGGATGGTGAAGAGCTGGGACCAGGGCACCCCTCCCCTGGAGGTATTAGCTGAAAGAGAGAGTC
  TTTACAGGCTGTTACCTCAGACAACCCCAGAGAACATCAACAAAAATTTTGAGCAGTACACATTGGACCC
  AGCCACGCGGTACCCAAACCTCAACCTGCACTGCGTCAGGCCTCACCAGGTGAAGCATTTGTACATCACT
  AAGGAGATGGACCGCTTCCCTCTCGAGAGATGGGGCTCAGTGAGGAGATCTGTCAGCCTCTCCAGGCGGG
  AGTCAGACATCCGGCCTAACAAGCTTTTAACCTGGTGCCAGCAGCAGACCAAGGGTTACCAGCACGTCAG
  AGTCACTGACCTGACCACATCCTGGCGCAGCGGCTTGGCCCTGTGTGCCATCATCCACAGCTTCCGGCCA
  GAGCTGATCAACTTTGACTCGCTGAATGAAGATGACGCTGTGGAGAACAACCAACTGGCATTTGATGTGG
  CCAAGCGTGAGTTTGGGATCCTGCCTGTGACCACAGGCAAAGAGATGGCATCTACCCAGGAGCCAGACAA
  GCTCAGCATGGTCATGTACCTCTCCAAGTTCTATGAGCTCTTCCGGGGCACTCCACTGAGACCCATGGAT
  TCCTGGCGTAAAAACTATGGAGAAAATGCTGACTTTGGCTTGGGCAAAACATTCATTCAGAATAACTATC
  TCAACCTCACATTGCCCAGAAAGAGGACCCCACGGGTAGACACCCAGACTGAAGAGAATGACATGAACAA
  GAGGCGGCGACAAGGCTTCAACCACCTGGAAGAGCTGCCATCCTTCTCCAGCCGCAGCCTGGGCTCCAGT
  CAAGAGTATGCTAAAGAAAGTGGCAGTCAGAACAAGGTCAAGCACATGGCCAATCAGCTGCTAGCCAAGT
  TTGAGGAGAACACTCGGAACCCTTCAGTCGTGAAACAGGACTGCCGCCGGGTCTCAGGCATAGGTAAGCC
  TGTCCTGTGCTCTGCCTCTCGCCCTCCTGGCACCTCTTGCTGCCCCAAGCTGGAGGAGTCCACTCCCAGG
  CTTCCACCTCCTCTGAAAAGGCAGTTCTCCTCCACGGTGGCAACAGGACAGGTGCTCAGAGAACTCAACC
  AAGTACCGGCTAGTGGCGAGTGCCCCAGCAGACCCTGGAGAGCCAGGGCCAAGTCAGACCTGCAACTGGG
  TGGGGTTGAAAATCTCGCTACCCTGCCTCGCACCTGCCAAGGGGCACTGGCCCTGTCCGGGGTGCTGCGG
  CGCCTACAGCAAGTAGAAGAAAAGGTGCTTCAGAAGAGGGCCCAGAACTTGGCTAACAGGGAATTTCACA
  CAAAGAACATTAAAGAGAAGGCAGCTCACCTGGCCTCCATGTTTGGACACGGGGATTTACCACAGGATAA
  GCTCCTATCTAAACGCGTGCCTCACGCTCATCCTCCATCTCCTCCCTCTTGCCTTCCGTCTCCTCATCCA
  GCTGCTGCTTCCTCTCCACCCGCTGCCGACTCTGTTTCTCCTGCCAGAAAGCTGACAGTAGGGAAAGTAT
  CCAGCGGAATAGGGGCTGCAGCTGAAGTTCTGGTCAATCTGTACTTGAATGATCACAGACCTAAGACACA
  GGCCACCTCTCCAGACCTGGAATCCCCGAGAAAGGCATTCCCCCTGAGCCTGGGCGGCAGAGACACCTGC
  TACTTTTGTAAGAAGCGTGTATACATGATAGAGCGGCTGAGTGCTGAGGGCCACTTTTTCCACCAAGAGT
  GCTTCCGTTGCAGCGTCTGCAGTGCCACCCTGCGCCTGGCTGCCTATGCCTTTGACTGCGATGAAGGCAA
  ATTTTACTGCAAGCCCCATTTTGTTCACTGCAAAACCAGTAGCAAACAGCGAAAGAGACGGGCAGAGCTG
  AATCAGCAAAGAGAGGAGGAAGGAACATGGCAGGAGCAGGAAGCACCTCGGAGGGATGTACCCACAGAAA
  GCTCTTGTGCAGTGGCGGCCATCAGCACGCCAGAAGGCAGCCCCCCAGTACGTTTCAGCCTTCCAGTCCT
  ACACCCACTTCTTGGCTGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001193305
• Insert Size: 3309 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001193305.1, NP_001180234.1
• RefSeq Size: 3680 bp
• RefSeq ORF: 3309 bp
• Locus ID: 320878
• UniProt ID: Q8BML1
• Cytogenetics: 7 F1
• Gene Summary: Nuclear monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin to form methionine-sulfoxide, resulting in actin filament disassembly and preventing repolymerization (PubMed:23911929, PubMed:23927065). In the absence of actin, it also functions as a NADPH oxidase producing H(2)O(2) (By similarity). Acts as a key regulator of the SRF signaling pathway elicited by nerve growth factor and serum: mediates oxidation and subsequent depolymerization of nuclear actin, leading to increase MKL1/MRTF-A presence in the nucleus and promote SRF:MKL1/MRTF-A-dependent gene transcription. Does not activate SRF:MKL1/MRTF-A through RhoA (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (A) encodes the longer isoform (A). Sequence Note: This RefSeq record was created from genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.