Nrxn3 (NM_172544) Mouse Untagged Clone

CAT#: MC223549

Nrxn3 (untagged) - Mouse neurexin III (Nrxn3), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nrxn3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223549 representing NM_172544
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGGCTCAGATGACTTCTTCTATGTTGGTGGAAGCCCAAGTACCGCAGACTTGCCAGGTTCACCTG
  TGAGCAACAACTTCATGGGCTGCCTTAAAGAGGTTGTTTATAAGAATAATGACATCCGTCTAGAGCTGTC
  TCGCTTGGCCCGGATTGGAGACACCAAGATGAAAATCTATGGTGAAGTTGTGTTCAAGTGTGAGAACGTG
  GCCACACTGGATCCCATCAACTTTGAGACCCCAGAGGCTTACATCAGCTTGCCCAAGTGGAACACCAAAC
  GTATGGGCTCCATTTCCTTTGACTTTCGCACCACTGAGCCCAACGGCCTGATTCTCTTCACTCACGGGAA
  GCCTCAAGAAAGGAAAGATGTCCGGAGCCAAAAGAACACAAAAGTTGACTTCTTTGCTGTGGAACTTCTT
  GATGGCAACCTGTATTTGCTGCTTGACATGGGCTCTGGCACCATCAAAGTGAAGGCCACTCAGAAGAAGG
  CCAATGATGGGGAATGGTACCACGTGGATATTCAGCGTGATGGCAGATCAGGTACCATATCTGTGAACAG
  CAGGCGCACGCCATTCACCGCCAGTGGGGAGAGTGAAATTCTCGACCTGGAGGGGGACATGTATCTGGGC
  GGGCTGCCAGAGAACCGAGCTGGCCTCATCCTCCCCACGGAGCTCTGGACCGCCATGCTCAACTACGGTT
  ACGTGGGCTGCATCCGCGACCTGTTCATCGATGGGCGCAGCAAGAACATCCGGCAGCTGGCGGAGATGCA
  GAACGCAGCAGGCGTCAAGTCCTCCTGTTCACGCATGAGCGCCAAGCAGTGTGACAGCTACCCCTGCAAG
  AACAACGCGGTGTGCAAGGACGGCTGGAACCGCTTCATCTGCGACTGCACTGGCACCGGCTACTGGGGGA
  GAACCTGCGAAAGAGAGGCATCTATCCTGAGCTATGACGGCAGCATGTACATGAAGGTCATCATGCCCAT
  GGTGATGCACACGGAGGCAGAGGATGTGTCCTTCCGCTTCATGTCACAGCGAGCCTATGGGCTACTGGTA
  GCCACAACCTCCAGGGATTCTGCAGATACACTGCGTCTAGAGTTGGATGGTGGGCGTGTCAAGCTCATGG
  TTAACTTAGACTGTATCAGGATAAACTGTAACTCCAGCAAAGGACCTGAGACCCTGTATGCAGGGCAGAA
  GCTCAATGACAATGAGTGGCACACCGTCCGGGTAGTGCGGAGAGGAAAAAGCCTTAAGCTAACAGTGGAT
  GATGATGTGGCTGAGGGGACAATGGTGGGCGACCACACCCGCCTGGAATTCCACAATATTGAAACTGGTA
  TCATGACTGAGAAGCGTTACATCTCTGTGGTCCCCTCCAGTTTCATTGGCCATCTACAGAGCCTCATGTT
  CAATGGCTTACTGTACATTGATTTGTGCAAAAATGGCGACATCGACTACTGTGAACTGAAGGCTCGCTTT
  GGACTGAGAAACATCATCGCCGACCCTGTCACTTTTAAGACCAAGAGCAGCTACCTGACCCTCGCCACCC
  TACAGGCTTACACCTCCATGCACCTCTTCTTCCAGTTCAAGACCACTTCAGCTGATGGCTTCATTCTCTT
  CAATAGCGGAGATGGCAATGATTTTATTGCAGTTGAGCTGGTCAAGGGGTACATACACTATGTGTTTGAT
  CTCGGCAATGGTCCCAATGTGATCAAAGGCAACAGTGACCGCCCCCTGAATGACAACCAGTGGCACAACG
  TGGTCATCACCAGGGACAGCAGTAACACCCACAGTCTGAAGGTGGACACCAAGGTAGTCACTCAGGTCAT
  CAATGGTGCCAAAAATCTGGATTTGAAAGGTGACCTCTATATGGCTGGCTTAGCCCAGGGCATGTACAGC
  AACCTTCCCAAGCTTGTGGCCTCCAGGGATGGATTTCAGGGCTGCCTGGCCTCTGTGGACTTGAATGGAC
  GCCTGCCTGATCTCATCAACGATGCTCTCCACAGGAGTGGACAGATCGAGCGAGGCTGTGAAGTTGCGTT
  GACCAAAGCTGACCTGCAAGGACCCAGTACAACCTGTCAGGAGGATTCATGCGCCAACCAGGGAGTTTGC
  ATGCAGCAGTGGGAAGGTTTCACCTGTGACTGCTCCATGACATCATATTCTGGAAACCAGTGCAATGACC
  CTGGTGCAACATACATCTTTGGGAAAAGCGGTGGCCTCATCCTCTATACCTGGCCAGCAAATGACAGACC
  CAGCACACGCTCTGACCGTCTCGCCGTGGGCTTCAGCACTACTGTGAAGGATGGTATCTTAGTACGCATT
  GACAGCGCCCCTGGACTTGGCGACTTCCTCCAGCTTCACATAGAACAAGGGAAAATTGGAGTTGTCTTCA
  ATATTGGCACAGTTGACATCTCCATCAAAGAAGAGAGAACTCCTGTCAATGATGGCAAATACCACGTTGT
  GCGCTTCACCAGGAATGGGGGAAATGCTACACTTCAGGTGGACAACTGGCCAGTGAATGAGCACTATCCT
  ACAGGCAACACTGATAATGAACGCCTCCAAATGGTAAAACAGAAAATCCCCTTCAAATATAACCGGCCCG
  TAGAGGAGTGGCTGCAGGAAAAAGGCCGGCAGCTAACCATCTTCAACACCCAGGCGCAAATAGCCATCGG
  AGGAAAGGACAAAGGACGTCTCTTCCAAGGTCAACTCTCTGGGCTCTATTATGATGGTTTGAAAGTACTG
  AACATGGCAGCTGAGAACAACCCTAATATTAAAATCAATGGAAGTGTCCGGCTAGTGGGAGAAGTCCCAT
  CAGTCTCAGGAACAACACAGACAACGTCCATGCCACCTGAAATGTCTACCACCGTCATGGAAACCACCAC
  CACCATGGCTACGACCACCACCCGAAAGAACCGCTCTACAGCCAGCATTCAGCCCACGTCAGATGATCTT
  GTTTCATCTGCTGAATGTTCAAGTGATGATGAAGACTTTGTCGAATGTGAACCAAGTACAGCAAACCCCA
  CGGAGCCAGGAATCAGACGGGTTCCGGGGGCCTCAGAGGTGATCCGGGAGTCCAGCAGTACAACAGGGAT
  GGTCGTCGGCATTGTGGCTGCTGCCGCCCTCTGCATCTTGATCCTCCTGTACGCCATGTACAAGTACAGG
  AACAGGGACGAGGGGTCCTATCAAGTGGACGAGACGAGGAACTACATCAGCAACTCGGCCCAGAGCAACG
  GCACGCTCATGAAGGAGAAGCAAGCCAGCTCCAAGAGCGGCCATAAGAAACAGAAAAACAAGGACAAGGA
  GTATTATGTGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172544
• Insert Size: 3303 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172544.3, NP_766132.2
• RefSeq Size: 6529 bp
• RefSeq ORF: 3303 bp
• Locus ID: 18191
• UniProt ID: Q6P9K9
• Cytogenetics: 12 42.94 cM
• Gene Summary: This gene encodes a member of a family of proteins that function in the nervous system as receptors and cell adhesion molecules. Extensive alternative splicing and the use of alternative promoters results in multiple transcript variants for this gene, but the full-length nature of many of these variants has not been determined. Transcripts that initiate from an upstream promoter encode alpha isoforms, which contain epidermal growth factor-like (EGF-like) sequences and laminin G domains. Transcripts initiating from the downstream promoter encode beta isoforms, which lack EGF-like sequences. [provided by RefSeq, Dec 2012]
  Transcript Variant: This variant (2) differs in the 5' UTR and contains multiple differences in the coding region, including the lack of multiple 5' exons, compared to variant 1. It initiates translation at a downstream in-frame start codon. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1.