Mkl2 (NM_153588) Mouse Untagged Clone

CAT#: MC223494

Mkl2 (untagged) - Mouse MKL/myocardin-like 2 (Mkl2), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mkl2
• Synonyms: ENSMUSG00000075401; Gt4-1; mKIAA1243; MRTF-B; Mrtfb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223494 representing NM_153588
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCGATAGCTCCAAGAAGCAGCCACAAGGCTTCCCAGAAATTTTAACTGCTGAGGATTTTGAACCTT
  TCAAAGAAAAGGAATGCCTTGAGGGAAGCAACCAGAAAAGTCTCAAGGAAGTGCTCCAGCTGAGGCTGCA
  ACAAAGGAGGACAAGAGAACAACTAGTGGACCAGGGCATCATGCCACCTTTGAAGAGTCCAGCAGCATTC
  CATGAGCAGATAAAAAGCCTGGAGCGAGCCAGAACTGAGAATTTTCTGAAGCACAAGATCCGGAGCCGCC
  CAGACCGCTCTGAGCTTGTCAGGATGCATATTTTAGAAGAAACGTTTGCAGAGCCATCTCTTCAGGCCAC
  ACAGATGAAGCTGAAGAGAGCTCGGCTGGCAGATGACCTGAACGAGAAGATTGCTCAAAGGCCTGGTCCC
  ATGGAGCTGGTGGAGAAGAACATCCTTCCTGTGGATTCGAGTGTCAAGGAAGCGATTATAGGTGTGGTGA
  AGGAGGACTATCCTCACACTCATGGCGAGTTCTCATTTGATGAAGATAGCAGTGATGCTTTGTCTCCAGA
  CCAGCCAGCGAGCCAGGAGTCTCAGGGCTCAGCTGCATCCCCCAGTGAGCCAAAAGTCAGTGCCTCGCCA
  CCTCCTGTGACTGCAAGCACTCCAGCCCAGTTTACCTCAGTGTCCCCAGCAGTTCCTGAATTCTTGAAAA
  CCCCTCTAACTGCAGACCAGCCTCCCACAAGGTCTACAGCTCCCGTGCTCCCTACAAACACTGTGTCCTC
  AGCGAAGTCTGGCCCAATGCTGGTGAAGCAAAGCCATCCCAAGAATCCAAACGACAAACACCGTAGCAAA
  AAATGCAAAGATCCGAAACCGCGAGTGAAGAAGCTTAAGTACCACCAGTACATTCCACCCAACCAGAAGG
  GGGAGAAGAGCGAGCCACAGATGGACTCCAACTATGCCCGCCTGCTCCAGCAGCAGCAGCTCTTCCTACA
  GCTGCAGATCCTGAGCCAGCAGCAGCAGCAACAGCAGCAGCAGCACTACAACTACCAGACCATCCTACCT
  GCACCCATCAAAACTGACAAGAACAGCAGCAGCGGCAGTAACAGCGGCAGTAGCAGCAGCATGCCGGCGA
  GGAGGCCAGGACCGCTGCCCTCCAGCTTGGATGACTTAAAGGTGTCAGAGCTGAAGACAGAACTCAAACT
  GAGGGGTCTGCCAGTGTCAGGAACTAAACCAGACCTCATTGAACGCCTGAAACCCTACCAGGAAGTGACC
  AGTAGTAACCTTGCCACTGGCAGTATTGTGGCAGTGTCATCAGCAACCATTGTCACCAGTAACCCAGAGG
  TCACAGTGGCACTGCCAGTCACAACACTGCATAATGCTGTAACAAGCTCTGTGTCCACTTTCAAGGCAGA
  CTTGGCACTTCCTGCAACCAGCAGTGTGCCCCATGTTGAAAATGCCCACTCTCCTCTGCCCATCTCCCCA
  TCACCCTCTGAGCAGTCCAGTCTCAGTACCGATGACACAAACATGACAGACACCTTCACAGAGATCATGA
  CTATGATGTCACCTTCCCAGTTGTTGTGTTCATCTCCCCTGAGGGTGGTGAGCCATGATGACAGCCTGAG
  CCCTTCCAGTAGCACCCTGTCTACTCTAGAACTGGATGCTGCTGAGAAGGACCGCAAACTTCAGGAGAAG
  GAGAAACAGATTGAAGAACTGAAGAGGAAACTGGAACAAGAACAGAAGCTTGTGGAGGTCCTCAAGATGC
  AGCTTGAGGTGGAAAAACGTGGGCAGCAGCGACCACCAGACCCTCAGCCCAGTGATCCCCCACATCCCTT
  TAATACATCAGATCCTAAACATGGCAGTGTTGGGTCCTCCATCAAGGATGAGGCCTCACTCCCTGACTGC
  TCCAGCCCCCAGCAGCCCATCACAGTGCCTGGCCACTCTGTAGGCCAGCCCATCTCCACGGGTAGCCAGA
  CCCTTGTTGCCAAAAAGACTGTGGTCGTCAAGCAAGAGGTCCCCATGGCCCAAGCAGAGCAGCAGAACGT
  TGTCTCGCAGTTCTACCTTAGTTCCCAGGGACAGCCACCTGCCCTTGTTGCTCAGCCTCAGGCCTTACTG
  ACCACACAGACAACTCAGCTGCTGCTGCCAGTGTCCATCCAGGGCTCAAATGTTACTTCAGTGCAACTCC
  CAGTAGGCAGCCTTCAACTCCAGACCCCAGCACAAGGAAGAGTCCAGGCTCAGCCTCATGTAGCTGCTGC
  CACACAGGTTCCAGCTGCAGCCCTGCCCTCAGCCTTGACCTCAGCACTGCCTCAGAAGCAGGAAGCCTTC
  CCACAGCACGTGTTGGGCCAGCCTCAGCCAGTCAGAAAGGTCTTCACAAACTCGGCACCAAACACAGTTC
  TTCAGTATCAGAGGCAGCCTGGCCCAACGAACCAGCAGCCCTTTGTCAGTAAGACCTCTAACCCTGCTCT
  TCAATCCAGAACTGCCCCACTTGCACCCCTGCAAAATGGACCCAGCCTAGCCAGCAAGCCTAGCTCACCA
  CCCCCACCCCAGCAGTTTGTTGTTCAGCACTCTTTGTTTGCAACCCCAATCACGAAGACAAAAGATCCAC
  CTCGATATGAGGAAGCCATCAAGCAGGCACGTAGCACACAGCCAGCCCTTCCAGAGGTTTCCAGTGTGCA
  CAGTCAGCAGATGGACGACCTCTTCGACATCCTCATAAAGAGTGGAGAGATTTCCTTCCCAATAAAAGAA
  GAGCCTTCTCCAATTTCCAAGATGAAACCAGTGACAGCCAGCATCACCACAATGCCAGTCAATACCGTGG
  TCTCTCGACCACCGCCCCAGGTCCAAATAGCACCACCTGTATCCTTAGAACCTGTGAACAGTTTATCTGC
  CAGCTTAGAGAACCAGCTAGAAGCCTTCTTGGATGGCACTTTGCCCTCGGCCACTGACACTGGCCCACTG
  CAGAACAGCAGTGAAGATAGAGAGTCTTTCTCTCTGATCGAGGATCTGCAGAACGACTTGCTGAGTCATT
  CCAGCATGCTGTACCAGTCTCACTCTCCCATGGAGACCTCTGAGGCCCAGTTGGTGTCAGGGACCCCCTG
  TCTATCTCTTGACCTGTCAGACTCCAACCTGGACAACATGGAGTGGCTAGACATTACCATGCCCACCACA
  TCCTCCGGGCTCACTCCACTGAGTACCACTGCGCCAAGCATGTTCTCTGCTGATTTTCTGGACCCACAGG
  ACCTGCCGCTGCCATGGGACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_153588
• Insert Size: 3243 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153588.3, NP_705816.2
• RefSeq Size: 8295 bp
• RefSeq ORF: 3243 bp
• Locus ID: 239719
• UniProt ID: P59759
• Cytogenetics: 16 A1
• Gene Summary: Acts as a transcriptional coactivator of serum response factor (SRF). Required for skeletal myogenic differentiation.[UniProtKB/Swiss-Prot Function]