Uba1y (NM_011667) Mouse Untagged Clone

CAT#: MC223394

Uba1y (untagged) - Mouse ubiquitin-activating enzyme E1, Chr Y 1 (Ube1y1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Uba1y
• Synonyms: A1s9Y-1; Sby; Ube-2; Ube1ay; Ube1y; Ube1y-1; Ube1y1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223394 representing NM_011667
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCAGCTCAGTCCTGTCCAAGAAACGCAAAGTGTCTGGACCTGACTCTAGTCTGGATTCTAGTTGGT
  CTCCTACCTATTCTGTGATGTTTGGCGTGCCCCCAGGGCCAACCAACGAAATGTCAAAAAATAAGGAAAT
  GGACATAGATGAAAGCCTTTACTCCCGCCAGCTGTATGTACTCGGCCATGAGGCAATGAAGCATCTCCAG
  GCTTCCAGTGTACTGATATCAGGCCTGCAGGGTCTGGGTGTGGAAATTGCCAAGAATATCATCCTTGGTG
  GGGTCAAGGCTGTCACTCTCCATGATCAGGGCATTGCCCAGTGGGCTGATCTGTCTTCCCAGTTCTGCCT
  ACGTGAGGAAGATATTGGTAAAAACCGAGCTGAGATATCCCAACCACGCCTTGCTGAACTCAACAGTTAC
  GTTCCTGTGTTTGCCTACACTGGACCTCTAATTGAGGAGTTCCTTAGTGGTTTTCAGGTAGTGGTTCTTA
  CCAACACTCCTTTGGAATATCAGCTGCAGGTGGGTGAATTCTGCCATAGCCACGGAATCAAGCTGGTAGT
  AGCAGACACTCGGGGCCTAGTTGGACAACTCTTCTGTGACTTTGGAGAGGAAATGATTCTCACTGATTCA
  AATGGAGAACAGCCACTCAGTGCCATGGTTTCAATGATCACTAAGGAGAATCCAGGGATTGTCACCTGCC
  TGGAGGACTCCCGGCATGGATTTGAAAGTGGTGACTTTATCTCTTTCACAGAAGTTCAGGGCATGAGTGA
  ACTCAATGGCATTGGTCCCATAGAGATCAAAGTTCTGGGTCCCTATACCTTTAGTATCTGTGATACCTCC
  AGCTTCTCTGAGTACATCCGTGGAGGCATTGTCAGTCAAGTGAAAGTACCTCGGAAGATCAATTTTAAAC
  CCCTACTTGCCTCGCTGGCAGAGCCAGAGTTTGTGGTCACAGATTTTGCTAAGTGCTGTCATCCTGCTCA
  GCTCCACATTGGCTTCCAGGCCCTGCATCAGTTCTGTACTCAGCACAGCAGGCCTCCTCGGCCCCATAAT
  GAGGAGGATGCTGAGGAATTGGTGACCTTAGCCCAATCTGTGAATGCTCAAGCTTTGCCAGCAGTTCAGC
  AGGATTGCCTGGACATCGACCTTATCCGGAAGTTGGCCTATGTAGCAGCTGGGGACCTGGCACCCATGAA
  TGCCTTTTTTGGTGGTTTGGCTGCCCAGGAGGTCATGAAGGCTTGTTCTGGAAAGTTTATGCCCATTAGG
  CAGTGGCTGTACTTTGATGCCCTTGAATGTCTCCCAGAGCACAGAGTGGCCTTCATGGAAGATAAGTGCC
  TGCCACACCAGAACCGTTATGATGGGCAAGTGGCTGTATTTGGATCAGACCTACAAGAGAAGCTTGGCAA
  GCAGAAATACTTCCTGGTAGGTGCAGGTGCCATTGGCTGTGAGCTGCTCAAGAACTTTGCCATGATTGGC
  CTCGGCTGTGGGGAGGATGGAGAAATCACAGTTACAGACATGGACACCATTGAGAAGTCAAACCTGAACA
  GGCAGTTTCTCTTCCGTCCCTGGGACATCACAAAATTAAAGTCTGAGACTGCTGCTGCAGCAGTGCGTGA
  CATAAATCCACACATCAGGATATTCAGCCACCAGAATCGAGTAGGCCCAGAGACAGAACACGTCTATGAT
  GATGACTTCTTCCAAAAGCTGGATGGTGTGGCCAATGCCCTAGACAATGTGGATGCTCGATTATACGTGG
  ACCGCCGTTGTGTTTACTATCGTAAGCCTCTGCTGGAATCAGGCACGCTGGGCACCAAGGGCAACGTGCA
  GGTGGTTGTTCCCTTCTTGACAGAATCTTACAGCTCAAGCCAGGACCCACCTGAGAAATCCATCCCCATC
  TGCACACTGAAGAACTTCCCTAATGCCATTGAGCACACCGTGCAGTGGGCTCGGGATGAGTTTGAAGGGC
  TCTTCAAGCAGTCAGCTGAAAATGTTAACCAATACCTCACGGACCCCAAGTTCATGGAGCGGACACTGCA
  GCTAGCCGGCACCCAGCCTTTGGAAGTACTGGAGGCCATACACTGCAGCCTGGTCCTGCAGAGGCCACAG
  ACTTGGGCCGACTGTGTGACTTGGGCCTACCAGCACTGGCACACCCAGTATTCTCACAACATCCAGCAGT
  TGCTGCACAACTTCCCTCCAGCACAGCTTACGAGCTCTGGAGCACTTTTTTGGTCAGGACCAAAACGCTG
  TCCACATCCTCTCACCTTTGACATAAACAATCCCCTGCATCTGGATTATGTGATGGCTGCTGCCAACCTG
  TTTGCTCAGACATACGGACTAGGAGGGTCCCAGGACTGTGCTGTGGTGGCCAAACTCCTGCAGTCTCTGC
  CAGTCCCCAAGTTTGCTCCCAAGTCTGGCATCAGGATCCATGTTTCTGAGCAGGAGCTGCAGAGTACCAG
  TGCCACCACCATTGATGACAGCCACCTGGAGGAACTCAAGACTGCACTTCCTACTCCAGACAAGCTGCTT
  GGATTCAAGATGTACCCCATTGACTTTGAGAAGGATGACGACAGCAACTTTCACATGGATTTCATTGTGG
  CAGCATCCAACCTCCGAGCAGAAAACTATGGCATTTCCCCAGCAGACCGGCATAAGAGCAAACTGATTGC
  AGGCAAGATCATCCCAGCCATTGCAACCACCACATCTGCTATAGTTGGCCTTGTGTGTCTGGAGCTGTAC
  AAGGTGGTTCAGGGCCACCAACAACTGGAGTCTTATAAAAACAGTTTTATCAACTTGGCTCTGCCTTTGT
  TTAGCTTTTCTGCACCTCTGGCTCCAGAGTGTCATCAGTACTATGATCAAGAGTGGACACTGTGGGATCG
  CTTTGATGTGCAAGGACTGCAACCTAGCGGTGAGGAGATGACCTTGAAGCAGTTTCTAGACTACTTTAAG
  ACAGAGCACAAGCTGGAAGTCATCATGCTGTCCCAGGGTGTGTCCATGCTCTATTCTGTCTTTATGCCAG
  CCAGCAAGCTCAAAGAACGGTTGGATCAGCCGATGACAGAGATTGTGAGCTGTGTGTCAAAGCAAAAACT
  GGGCCATCATGTGAAGTCACTTGTGTTTGAGCTGTGCTGCAACAGTGACAGTGGAGACGACATAGAGGTT
  CCTTATGTACGATACATCATCCGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011667
• Insert Size: 3177 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011667.2, NP_035797.1
• RefSeq Size: 3981 bp
• RefSeq ORF: 3177 bp
• Locus ID: 22202
• UniProt ID: P31254
• Cytogenetics: Ypter
• Gene Summary: Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP (By similarity). The Y chromosome form could be involved in the survival and proliferation of differentiating spermatogonia.[UniProtKB/Swiss-Prot Function]