Dsg1a (NM_010079) Mouse Untagged Clone

CAT#: MC223389

Dsg1a (untagged) - Mouse desmoglein 1 alpha (Dsg1a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dsg1a
• Synonyms: DG1; DGI; Dsg; Dsg1; dsg1-alpha
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223389 representing NM_010079
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTGGCACTCCTTCAGGATAGCTGCCCTGCTGCTCACTTCCCTGGTGGTGCTTGAAGTTAACAGTG
  AATTTCAAATCCAGGTAAGAGATCATAACGCCAAAAATGGTACCATCAAGTGGCATTCCATCAGAAGGCA
  GAAACGAGAATGGATCAAGTTTGCTGCAGCCTGTCGCGAAGGTGAAGACAACTCAAAGAGAAACCCAATT
  GCCAAAATTCATTCAGATTGTGCTGCAAATCAACCAGTGACATACCGCATCTCGGGAGTGGGAATTGACC
  AGCCACCTTATGGAATCTTCATTATTAATCAAAAAACTGGTGAAATCAATATAACATCCATTGTTGATCG
  TGAAGTCACCCCCTTTTTCATTATCTACTGCCGAGCACTGAATGCACAAGGTCAAGACTTGGAGAATCCA
  CTGGAGCTTAGAGTTAGAGTGATGGATATAAATGACAACCCTCCTGTGTTTTCAATGACTACATTTCTAG
  GACAAATAGAAGAAAATTCTAATGCAAACACACTGGTAATGAAACTCAATGCTACCGATGCCGATGAGCC
  AAATAACTTGAACTCAATGATAGCCTTCAAGATCATAAGACAGGAGCCTTCTGACTCACCAATGTTCATC
  ATCAACAGGAAAACTGGAGAAATCAGAACAATGAATAACTTTCTAGACAGAGAGCAATATAGCCAGTATT
  CCCTTGTGGTGAGAGGCTCAGACCGGGACGGTGGAGCAGACGGCATGTCTGCAGAGAGCGAGTGCAGCAT
  CACCATCCTGGATGTCAATGACAACATCCCATACTTGGAGCAGTCATCTTATGACATCACAATTGAAGAA
  AATGCACTTCACTCACAGCTAGTGCAGATCAGAGTCATTGATTTGGATGAAGAATTCTCAGACAACTGGA
  AGGCAATCATTTTCTTTATCTCTGGAAATGAGGGAAATTGGTTTGAAATAGAAATGAATGAAAGAACAAA
  TGTAGGAACTCTGAAGGTTGTCAAGCCCCTAGATTATGAAGCCATGAAGAATCTACAGCTTAGTATTGGT
  GTTCGGAATGTAGCTGAATTCCACCAGTCAATTATTTCTCAATACAGACTCACAGCAACTATGGTCACTG
  TGACTGTGTTAAATGTCATCGAGGGCTCTGTGTTCCGTCCAGGTTCAAAGACATTTGTAGTAGACAGTAG
  AATGGAAGCAAATCACAGAGTGGGAGAGTTTGTAGCTACAGACCTGGACACAGGTCGAGCTTCCACAAAT
  GTTAGATATGAAATGGGAAATAACCCAGAGAACCTGCTGGTTGTTGACTCAAGAACAGGCATAATAACGT
  TAAGAAACAGAGTTACTATGGAACAATACCAAAGGCTTAATGGGGAATATAAAGGAACAGTGCTATCAAT
  AGATGATTCCCTTCAGAGGACCTGCACGGGGACAATCGTCATTGAACTTAGTGGCACTGGCTGGGTAACT
  GGTTCAGAAAGTGGTGGCAGTTCCAGTGGCAGTGGCGATGATCGTGACCGAGTGACTAATGGATATCAAG
  GCACTTCTTCCACTGAGAACCCACAACGCGTCACAGGATCATGGGGAGGCAGCGGCATCGATGGCACTCG
  CCCTAACACTAACCCTTTCCAAGGGGATCCTGATGAAACCCTTGAGACCCCTCTCTATGGAGACAACGTT
  CACTTCGGTCCTGCAGGCATCGGGCTGCTCATCATGGGGTTCCTAGTCTTAGGATTGGTTCCATTCCTGC
  TGATCTGCTGTGATTGTGGAGGGGCCCCTGGTGGTGGAGCTGGATTTGAACCTGTTCCAGAATGTTCTGA
  TGGAGCAATTCACACGTGGGCTGTAGAAGGGCCACAGCCTGAACCCCATGAAGGGATAACCACCATCTGT
  GTGCCACAAATGCCACCTGGTAATGCCAATGTTATAGAATATATTGACAACTCAGGAGTTTATACAAATG
  AATATTGTGGCAGAGAAATGCAAGATCTGGGAGGAGGAGAAAGAACCACAGGATTTGAACTAATGGATGG
  AGTTAAAACATCAGCTGCACCTGAGATATGCCAAGAATATTCAGGAACATTAAGAAGAAATTCAATGAGA
  GAATGTAGAGATGGGGGTCTCAACATGAATTTCATGGAAAGTTACTTCTGCCAGAAAGCCTATGCTTATG
  CGGATGAAGATGAAGGACGTCCATCCAATGACTGTTTGCTCATCTATGACATCGAAGGTGTGGGTTCCCC
  TGCAGGCTCCGTGGGCTGTTGCAGTTTCATTGGAGAAGATCTAGATGAGAGCTTCCTGGATACCTTGGGG
  CCCAAGTTTAAGAAGTTGGCAGATATCAGCCTGGGAAAAGAAATAGACTCATACCCAGATTCTGATCCTT
  CTTGGCCTCCTCAGAGCACTGAACCGATGTGTCCCCAGCATACAGAGCCCCTGGGTAGTGGACACCCACC
  CATCTCCCCACATTTTGGCACGACCACAGTAATTTCTGAAAATGCCTACCACTCTGGACCTGGAGTACAG
  CATCCTGTACCAATTCCTGATCCTCTGGGCTATGGCAATGTCACTGTGAGGGAGTCCTATACCACCTCTG
  GCACTCTGAAACCCTCTGTCCACTTTCATGATAATCAGCAGGCTTCAAATGTGGTGGTGACAGAGAGGGT
  GGTGGGCCCCATCTCTGGTGCTGATTTGCATGGCATGTTAGAGATACCTGACTTAAGAGGTGGAGCAAAT
  GTTATAGTGACAGAAAGGGTAATAGCACCAGGCTCAAGTCTACCCACTTCTCTAACCATTCCTAACCCTC
  AAGAGACCTCAAATGTAGTTGTGACAGAAAGAGTGATCCAACCAACCTCTGGCATGATAGGCAATCTGAG
  CATGACCCCTGAATTATCAAGTGCCCACAACGTGATTGTGACAGAGAGAGTGGTTTCTGGTGCTGGTATG
  AGTGAAATTGCAGGCACTGCCGGATTAGGGGGGGTTGGAGGCATAGGCAGCAGTGGCCTTGTTAGCACCA
  CCATGGGTGCTTCTGGCACTGGGCTGAACATGGGAGGAACAGCCACTATTGGCCACATGAGGAGCTCCTC
  TGACCATCACTTTAGTCAGACAGTTGGATCTGCCTCCCCCAGCATGGCTCGAAGTCGAATCACAAAGTAC
  AATACGGTACAGTATAGCAAGTAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_010079
• Insert Size: 3174 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010079.2, NP_034209.2
• RefSeq Size: 5627 bp
• RefSeq ORF: 3174 bp
• Locus ID: 13510
• UniProt ID: Q61495
• Cytogenetics: 18 11.29 cM
• Gene Summary: This gene encodes a member of the cadherin family of proteins that forms an integral transmembrane component of desmosomes, the multiprotein complexes involved in cell adhesion, organization of the cytoskeleton, cell sorting and cell signaling. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional protein. This gene is located in a cluster of desmosomal cadherin genes on chromosome 18. [provided by RefSeq, Jan 2016]