Trp53bp1 (BC035206) Mouse Untagged Clone

CAT#: MC223081

Trp53bp1 (untagged) - Mouse transformation related protein 53 binding protein 1 (cDNA clone MGC:30704 IMAGE:3979746), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trp53bp1
• Synonyms: m53BP1, 53BP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC035206
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGAGATCAATGATCCTCTACTTGGGAATGAAAAAGGGGATTCTGAGTCTGCCCCAGAAATGGATG
  GAAAACTGTCTCTGAAAATGAAACTGGTTAGTCCTGAGACAGAGGCCAGTGAAGAATCTTTGCAATTTAG
  CCTGGAAAAGCCTACAACTGCTGAGAGAAAAAATGGATCTACTGCCATTGCAGAGCCTGTTGCCAGTCTC
  CAGAAGCCGGTGCCTGTGTTTGGCTGCATCTATGAAGCCCAGCAAGAGAAGGAGGCTCAAAGTGAGGCTC
  CTCCCTCTGCACCCGACAGGGCGAATTTGCTCCATTTTCCAAGTGCTCAAGAAGAAGACAAAGAAAGACC
  AGATGTTACCCCAAACCTCAGGCAGAGTGAACAGCCTGTGAAGCCTGTTGGGCCTGTCATGGACGATGCT
  GCTCCCGAGGACTCAGCTTCTCCTGTTTCGCAGCAGAGGGCCTCACAGGAGCCCTTCAGTCCTGCAGAGG
  ACGTGATGGAAACAGACCTGCTGGAAGGACTGGCTGCTAACCAGGACAGACCTAGTAAGATGTTGATGGA
  CAGGCCCACCCAGAGTAACATCGGGATCCAGACCGTGGACCATTCCCTGTGTGCCCCAGAAACTGTCTCG
  GCAGCAACGCAGACTGTGAAGAGTGTGTGTGAGCAGGGGACCAGTACAGCGGAGCAGAACTCTGGGAAAC
  AAGATGCCACTGTGCAGACTGAGAGGGGGAGTGGAGAGAAACCTGCCAGTGCTCCCGTGGACGACACAGA
  GTCCCTCCACAGCCAGGGGGAAGAGGAATTTGAAATGCCCCAGCCTCCGCATGGCCATGTCTTGCATCGC
  CACATGAGAACCATTCGAGAAGTCCGTACACTCGTCACCCGCGTCATCACAGATGTTTATTATGTGGATG
  GGACAGAAGTGGAAAGGAAAGTAACGGAGGAGACTGAAGAACCAATCGTAGAGTGTCAGGAATGTGAAAC
  AGAAGTTTCCCCTTCACAGACTGGAGGTTCCTCTGGGGACCTGGGGGACATCAGCTCCTTCTCCTCCAAA
  GCTTCCAGCTCACACCACACATCCAGTGGAACAAGTCTCTCAGCCATACACAGCAGTGGCAGCTCAGGGC
  GAGGAGCTGGGCCACTCAAAGGAAAAGCCAGTGGGACAGAAGCTGCAGATTTTGCCTTACCCAGTTCCCG
  AGGAGGCCCAGGAAAACTGAGTCCTAGAAAAGGGATCAGTCAGACAGGGGCACCGGTGTGTGAGGAAGAT
  GGTGATGCAGGCCTTGGCATCAGACAGGGAGGGAAGGCTCCAGTTACACCTCGTGGGCGTGGGCGAAGGG
  GCCGCCCACCTTCTCGGACCACTGGAACCAGAGAAACAGTTGTCTCTGGTCCGTTGGGCGTAGAAGATAT
  TTCACCTAGCATGTCACCAGATGACAAGTCCTTCACCCGCATTATGCCTCGTGTGCCAGATTCTACCAAA
  CGGACGGATGCCAGTTCTAGTACTTTGCGGCGGAGTGATTCTCCAGAGATTCCTTTTCAGGCTGCTACTG
  GTTCCTCTGATGGCTTGGATTCCTCATCTTCAGGAAACAGCTTTGTGGGTCTCCGTGTTGTAGCTAAGTG
  GTCATCCAATGGCTACTTTTACTCTGGGAAGATCACCCGAGATGTGGGGGCTGGGAAGTACAAGCTGCTC
  TTTGATGATGGGTACGAATGTGACGTGCTGGGCAAAGACATTCTCCTGTGTGACCCCATCCCCCTGGACA
  CTGAAGTGACAGCCCTCTCAGAAGATGAATATTTCAGTGCAGGAGTGGTGAAAGGACATAGGAAGGAGTC
  TGGGGAGCTGTATTACAGCATTGAAAAAGAAGGCCAAAGGAAGTGGTATAAACGAATGGCGGTCATTCTG
  TCCTTGGAGCAAGGAAACAGACTGAGAGAGCAATATGGGCTTGGCCCATATGAAGCTGTCACACCGCTCA
  CGAAGGCAGCAGATATCAGCTTAGACAATTTGGTGGAAGGAAAGCGGAAACGGCGCAGTAACATCAGCTC
  CCCAGTCACCCCCACTGCCGCCAGTAGCAGCAGCACAACGCCCACACGTAAAGCCACAGAGAGTCCCCGT
  GCTTCCACGGGAGTCCCGTCAGGCAAAAGGAAACTTCCGACTTCTGAAGAGGAACGCTCTCCTGCTAAGC
  GAGGTCGAAAGTCTGCCACCGTGAAACCTGGAACAGTGGGGGCAGCAGAGTTTGTGAGTCCCTGTGAGAC
  TGGAGACAACATAGGTGAGCCTTCTGTCCTAGAAGAGCCAAGAGGGCCTTTGCCCCTCAACAAGACCTTG
  TTTCTGGGCTATGCCTTTCTCCTCACCATGGCTACAACTAGTGACAAGCTGGCCAGTCGCTCTAAGCTGC
  TAGATGGTCCTACAGGAAGCAGTGAAGAAGAGGAGGAATTTTTAGAAATTCCTCCTTTCAACAAGCAGTA
  TACAGAATGCCAGCTTCGAGCAGGAGCTGGGTATATCCTTGAAGACTTCAATGAAGCCCAGTGTAACACA
  GCCTACCAGTGTCTCCTAATTGCGGACCAGCACTGTCGAACCCGGAAGTACTTCCTGTGCCTTGCCAGTG
  GCATTCCTTGTGTGTCTCATGTCTGGGTCCATGACAGTTGCCATGCCAACCAACTTCAAAACTATCGTAA
  TTATCTGCTGCCTGCTGGGTATAGCCTTGAAGAGCAACGAATTCTGGATTGGCAACCCCGTGAAAACCCT
  TTCCAGAATCTGAAGGTCCTCTTGGTGTCAGATCAACAACAGAACTTCTTGGAGCTCTGGTCTGAGATCC
  TCATGACTGGAGGGGCAGCCTCTGTGAAGCAGCACCATTCAAGTGCCCACAACAAAGACATTGCTTTAGG
  GGTATTTGATGTGGTGGTGACAGACCCCTCATGCCCAGCCTCGGTGCTCAAGTGTGCTGAAGCCTTGCAA
  CTGCCTGTGGTATCACAAGAATGGGTGATCCAGTGCCTCATTGTTGGGGAGAGAATTGGATTCAAGCAGC
  ATCCAAAATATAAACATGATTATGTTTCTCACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC035206
• Insert Size: 3045 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC035206, AAH35206
• RefSeq Size: 3441 bp
• RefSeq ORF: 3044 bp
• Locus ID: 27223
• Cytogenetics: 2 E5
• Gene Summary: Double-strand break (DSB) repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR) during antibody genesis (PubMed:15159415, PubMed:15077110, PubMed:20453858, PubMed:23333305, PubMed:26308889, PubMed:20362325). Plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1 (PubMed:23333305, PubMed:20362325). In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites. Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites. Required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs (PubMed:15159415, PubMed:15077110). Participates to the repair and the orientation of the broken DNA ends during CSR (PubMed:26308889). In contrast, it is not required for classic NHEJ and V(D)J recombination (PubMed:15159415). Promotes NHEJ of dysfunctional telomeres (By similarity).[UniProtKB/Swiss-Prot Function]