Sh3pxd2b (NM_177364) Mouse Untagged Clone

CAT#: MC222562

Sh3pxd2b (untagged) - Mouse SH3 and PX domains 2B (Sh3pxd2b), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sh3pxd2b
• Synonyms: fad49; G431001E03Rik; TSK4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222562 representing NM_177364
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGCCGCGGCGCAGTATCGTGGAGGTGAAGGTGCTGGACGTGCAGAAGCGGCGCGTGCCCAACAAGC
  ATTATGTCTACATCATCCGAGTCACGTGGTCCAGTGGCGCCACGGAGGCCATCTACCGGCGCTACAGCAA
  GTTCTTTGACCTCCAGATGCAGATGTTGGACAAGTTCCCCATGGAAGGAGGACAGAAAGACCCCAAGCAG
  AGGATCATTCCATTTCTGCCAGGCAAAATCCTCTTCCGCCGCAGCCACATCCGGGACGTAGCCGTCAAAC
  GCCTGATACCAATTGATGAATATTGTAAGGCCCTGATCCAGCTGCCCCCCTACATCTCTCAGTGTGATGA
  GGTTCTGCAGTTTTTCGAGACAAGACCTGAGGACCTGAATCCTCCCAAAGAGGAGCATATTGGGAAGAAG
  AAATCAGGGAATGACCCGACCTCAGTGGACCCTATGGTCCTGGAGCAGTATGTGGTAGTGGCAGACTATC
  AGAAGCAGGAGAGCTCAGAGATCAGCCTCAGTGTGGGCCAAGTAGTGGACATCATTGAGAAGAATGAGTC
  AGGTTGGTGGTTCGTCAGCACTGCTGAAGAGCAGGGCTGGGTCCCTGCAACCTGCCTGGAAGGCCAGGAT
  GGCGTGCAAGATGAGTTCTCCCTCCAGCCTGAGGAAGAGGAGAAATATACGGTCATCTACCCGTACACAG
  CTCGTGACCAGGATGAAATGAACCTAGAGCGAGGGGCTGTGGTGGAGGTCGTCCAGAAGAACCTGGAGGG
  CTGGTGGAAGATCAGGTACCAGGGCAAAGAGGGCTGGGCCCCTGCTTCCTACCTAAAGAAGAACAGTGGG
  GAGCCCTTGCCCCCAAAGCTTGGCCCCAGCTCACCTGCTCACTCGGGTGCCCTCGACCTGGATGGTGTTT
  CCCGGCATCAGAATGCTATGGGCAGGGAGAAGGAGCTGCTGAACAACCAGAGGGATGGCCGGTTTGAAGG
  TCGCCTGGTGCCCGATGGTGACGTTAAGCAGAGATCACCAAAGATGAGGCAAAGACCCCCTCCTCGCCGA
  GACATGACCATTCCTCGAGGCCTCAACCTGCCGAAGCCCCCCATCCCACCCCAGGTGGAGGAAGAGTACT
  ACACCATCGCTGAGTTCCAGACCACCATCCCAGATGGCATCAGCTTCCAGGCGGGCCTGAAGGTGGAGGT
  GATTGAGAAGAGCCTAAGTGGTTGGTGGTACATTCAGATGGAAGATAAGGAGGGGTGGGCCCCAGCGACC
  TTCATTGACAAGTATAAGAAGACCAGCAGTGCCTCGAGGCCCAACTTTCTGGCCCCCTTGCCTCATGAGA
  TGACCCAGCTCCGGCTTGGGGATGCAGCTGCAACAGAGAACAATACGGGTCCAGAAGCCGTGGGGCCCTC
  CAGACCCCTGCCTGAGGCACCACACGGTGCTGTGGACTCTGGGATGCTCTGGTCCAAAGACTGGAAAGGA
  GGAAAAGAGGCCCCAAGAAAGGCATCTTCAGACCTGTCTGCGTCAACAGGATATGAGGAGATCTCGGACC
  CCACACAGGAGGAGAAGCCCAGCCTCCCTCCACGCAAAGAGTCCATCATCAAGTCTGAAGAGGAGTTGCT
  GGAGAGGGAAAGACAGAAGATGGAGCCGCTCCGGGGCTCCTCCCCCAAGCCCCCTGGCATGATATTGCCA
  ATGATTCCAGCAAAGCATGCCCCGCTGGCCCGGGACAGCAGGAAACCAGAGCCCAAACTTGACAAGAGCA
  AGTTCCCGCTGAGAAATGACATGGGGTTAGAGTGTGGCCACAAGGTGCTGGCCAAGGAAGTGAAAAAGCC
  CAACCTCCGGCCCATCTCCAGGTCTAAAGCTGAGCTGTCCGAGGAGAAGGTGGACCCCACCTCCCAGAAT
  CTGTTCATGAAGTCTAGACCTCAGGTTAGGCCCAAACCAACTCCTTCCCCCAAGACAGAGCCAGCTCAGA
  GTGAAGACCACGTGGATATCTACAACCTCAGGAGCAAGCTCAGACCTGCCAAGTCTCAAGAGAAAGCCTT
  ATTGGATGGAGAAAGCCACCATGCTGCTGGGAGCCATGACACAGCCCTCAGCAGAAGCTTCCTTCCCGGG
  GAGGGACCTGGCCATGGCCAGGACAGGTCTGGCAGACAGGATGGGCTGAGTCCAAAGGAGACACCCTGCA
  GAGCCCCTCCCAGGCCAGCCAAGACCACAGACCCTGGGCCCAAGAATGTGCCTGTGCCTGTCCAAGAAGC
  AACCCTGCAGCAAAGACCTGTGGTCCCACCTCGAAGACCCCCACCCCCCAAGAAAACCTCCTCGTCACCA
  CTGTCGTGCAGGCCCCTTCCAGAGGTCAGAGGGGCACAGCGTGAAGAAAGCAGAGTCGCTCCTGCTGCAG
  GCCGGGCCCTCCTTGTCCCTCCAAAAGCCAAACCCTTCCTCTCTAACTCCTCAGTGGGCCAAGATGACAT
  GCGAGGCAAAGGTGGGCTGGGGCCACGCGTCACTGGCAAGGTGGGAGAAACCAGAGAGAAGGCGGCTTCC
  TTCCTCAATGCCGATGGCCCAAAGGACTCACTTTACGTGGCCGTGGCCAACTTTGAAGGAGATGAAGACA
  CCAGCAGCTTCCAGGAAGGGACAGTGTTTGAAGTCCGGGAGAAGAACAGCAGTGGCTGGTGGTTCTGCCA
  GGTCCTCAGTGGGGCTCCTTCCTGGGAAGGCTGGATTCCTTCCAACTACCTCCGGAAGAAACCCTAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_177364
• Insert Size: 2727 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177364.3, NP_796338.2
• RefSeq Size: 7432 bp
• RefSeq ORF: 2727 bp
• Locus ID: 268396
• UniProt ID: A2AAY5
• Cytogenetics: 11 A4
• Gene Summary: Adapter protein involved in invadopodia and podosome formation and extracellular matrix degradation. Binds matrix metalloproteinases (ADAMs), NADPH oxidases (NOXs) and phosphoinositides. Acts as an organizer protein that allows NOX1- or NOX3-dependent reactive oxygen species (ROS) generation and ROS localization. Plays a role in mitotic clonal expansion during the immediate early stage of adipocyte differentiation.[UniProtKB/Swiss-Prot Function]