Eps15 (NM_007943) Mouse Untagged Clone

SKU MC222461

Eps15 (untagged) - Mouse epidermal growth factor receptor pathway substrate 15 (Eps15), transcript variant 1, (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Eps15
• Synonyms: 2410112D09Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222461 representing NM_007943; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCTGCGGCAGCCCAGCTCTCCCTGACACAGTTGTCAAGTGGGAATCCTGTATATGAAAAATACTACA; GACAGGTTGAGGCAGGCAATACTGGAAGGGTGTTGGCGTTAGATGCTGCTGCATTCCTGAAAAAGTCAGG; GCTTCCAGACTTGATTCTTGGAAAGATTTGGGATTTAGCTGACACAGATGGCAAAGGTGTCCTGAGCAAA; CAAGAATTCTTTGTTGCTTTACGGCTTGTGGCATGTGCTCAGAATGGACTGGAAGTTTCACTGAGTAGCC; TAAGTCTGGCTGTTCCTCCACCAAGATTTCATGACTCCAGCAGTCCGTTGCTAACCAGTGGGCCCTCAGT; TGCTGAGCTCCCGTGGGCTGTAAAGTCTGAAGATAAAGCCAAATATGATGCAATTTTTGACAGTTTAAGC; CCAGTGGATGGATTTTTGTCTGGTGATAAAGTGAAACCAGTGTTGCTCAACTCTAAGTTACCTGTGGAAA; TCCTTGGAAGAGTTTGGGAGTTGAGTGATATTGACCACGATGGAAAGCTGGACAGAGATGAGTTTGCAGT; TGCCATGTTTTTGGTATACTGTGCACTGGAGAAAGAACCTGTGCCAATGTCCTTGCCTCCAGCCTTGGTG; CCACCTTCTAAGAGAAAAACGTGGGTTGTATCCCCTGCAGAAAAAGCTAAATATGATGAAATTTTTCTGA; AAACTGATAAGGATATGGATGGATATGTGTCTGGACTGGAGGTCCGTGAAACCTTCCTGAAAACAGGTTT; ACCTTCTGCCTTGCTAGCCCACATTTGGTCACTATGTGACACAAAGGGCTGTGGGAAGCTTTCAAAAGAC; CAGTTTGCCTTGGCTTTTCACTTAATCAATCAGAAGTTAATAAAAGGCATTGACCCTCCTCATAGTCTCA; CTCCTGAGATGATTCCACCATCAGACAGATCCAGTTTACAAAAGAACATCACAGGATCAAGTCCTGTTGC; AGATTTTTCTGCTATTAAGGAACTAGATACCCTTAACAATGAAATAGTTGACCTGCAGAGGGAAAAGAAC; AATGTGGAGCAGGACCTTAAAGAGAAGGAAGACACAGTTAAGCAGAGGACCAGTGAGGTTCAGGATCTTC; AAGATGAAGTTCAAAGGGAGAGTATTAATCTACAAAAACTGCAGGCCCAGAAGCAGCAGGTGCAGGAGCT; CCTGGGTGAACTGGATGAGCAGAAAGCCCAGCTGGAGGAGCAGCTCCAGGAAGTCAGGAAAAAGTGTGCT; GAGGAGGCCCAGCTGATTTCTTCCCTGAAAGCAGAAATAACTAGTCAAGAATCTCAGATCTCCAGTTATG; AGGAAGAACTGTTGAAAGCTAGAGAAGAACTAAGTCGCCTACAACAAGAAACAGCACAATTGGAAGAAAG; TGTGGAGTCAGGGAAGGCTCAGCTGGAACCTCTTCAGCAGCACCTACAAGAGTCACAACAGGAAATCAGC; TCAATGCAAATGAGATTGGAAATGAAAGATCTGGAAACTGATAATAACCAATCAAATTGGAGCAGTAGCC; CACAAAGCGTTCTTGTTAATGGTGCTACAGATTACTGTAGCCTCAGCACCAGCAGCAGTGAAACAGCCAA; CTTCAACGAACATGCTGAAGGCCAAAACAACCTAGAGTCTGAACCCACACACCAGGAGTCCTCAGTAAGA; AGTAGTCCTGAAATCGCACCTTCTGATGTGACTGATGAAAGTGAGGCTGTGACTGTGGCTGGTAATGAGA; AAGTTACTCCGAGATTTGACGATGACAAGCACTCAAAAGAGGAAGATCCATTTAATGTAGAATCAAGTTC; ACTGACAGATGCAGTTGCAGATACAAACTTGGATTTTTTCCAGTCTGATCCTTTTGTTGGCAGTGATCCT; TTCAAGGATGATCCTTTTGGAAAAATTGATCCATTTGGTGGTGACCCTTTCAAAGGCTCAGATCCTTTTG; CGTCTGATTGCTTCTTTAAGCAGACTTCTACTGATCCTTTTACCACTTCAAGTACGGACCCTTTCAGTGC; ATCCAGCAACAGCAGTAATACATCGGTAGAAACTTGGAAGCACAATGACCCATTTGCTCCTGGTGGAACA; GTTGTTGCTGCAGCGAGTGATTCAGCCACAGACCCTTTTGCTTCTGTTTTCGGAAATGAATCATTTGGAG; ATGGATTTGCTGACTTCAGCACATTATCAAAGGTCAACAATGAAGATGCTTTTAATCCTACCATATCAAG; TTCTACCAGCAGTGTGACCATTGCAAAACCTATGTTAGAGGAAACAGCCAGCAAGAGTGAAGATGTGCCT; CCAGCACTGCCGCCCAAAGTTGGCACTCCAACAAGACCTTGCCCGCCACCCCCTGGGAAAAGACCCATCA; ACAAATTGGATTCTTCTGATCCCCTTAAACTGAATGATCCATTTCAGCCTTTCCCAGGCAATGATAGTCC; CAAAGAAAAAGATCCTGATATGTTTTGTGATCCATTCACTTCTTCTACCACTACCAATAAAGAGGCTGAC; CCAAGCAATTTTGCTAACTTCAGTGCTTATCCCTCTGAAGAAGATATGATTGAATGGGCAAAAAGGGAAA; GTGAGCGGGAAGAAGAACAGAGGCTTGCCAGACTAAATCAGCAGGAGCAAGAAGACTTGGAACTGGCCAT; TGCA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_007943
• Insert Size: 2664 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_007943.3, NP_031969.1
• RefSeq Size: 2801 bp
• RefSeq ORF: 2694 bp
• Locus ID: 13858
• UniProt ID: P42567
• Cytogenetics: 4 C7
• Summary: Involved in cell growth regulation. May be involved in the regulation of mitogenic signals and control of cell proliferation. Involved in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Plays a role in the assembly of clathrin-coated pits (CCPs). Acts as a clathrin adapter required for post-Golgi trafficking. Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (A). Isoform A mainly localizes to clathrin-coated pits at the plasma membrane.