Gramd1b (NM_172768) Mouse Untagged Clone

CAT#: MC222348

Gramd1b (untagged) - Mouse GRAM domain containing 1B (Gramd1b), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gramd1b
• Synonyms: 3222402H23; A930008A22Rik; AI593249; mKIAA1201
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222348 representing NM_172768
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGCGGCCAACATGATGGAGAACCTGCAGCTGCCCGCCCTGCAGGTGCCAGAACCTCAGGGCGCAC
  CCGAGGGCAGCGCGGTGTGGTCCAGCTCGTCCACCCCCACGCTTCGCCGCCGGCGCTTTAAGATGCGCCG
  GATGAAGAACGTGCAGGAGCAGAGCCTGGAGGCGGGGTTGGTAGCCCCGGACCTACCCGGCGTCCTGGCC
  CCCGGCAAGGAGTTCCTGCAACTGCCGTCCATTGAGATTACGCCCTCCAGCGACGAGGACACCCCGTGGT
  CCAACTGCTCCACACCCAGTGCGTCCCCGCGCCGAAAACGCTTCCTGCTCCGCAAGTGGCTGAGGGTGAG
  GGAGCGGAAAGAATGCAGTGAAAGCAGCAGCCAGCAAAGCAGCCAACAGAGCAGCCACGACGATGATTCG
  AGCAGGTTCCTGAGTCCTCGAGTGCGCGACGAAAGCACTGCCAGTAACTCCAACCGCAGCACGCCAGCCT
  GCTCTCCCATCCTCCGGAAGCGGTCCCGCTCACCAACACCACAGAACCAGGACGGAGACACCATGGTGGA
  GAAGGGCTCAGATCACTCCTCGGACAAGTCCCCGTCCACCCCGGAGCAGGGTGTGCAACGCAGTTGCTCC
  TCACAGTCTGGTCGGAGCGGTGGTAAAAATTCCAAGAAAAGCCAGAGTTGGTACAATGTGCTAAGCCCCA
  CTTACAAGCAGAGAAATGAAGACTTCAGAAAGCTCTTTAAGCAACTTCCAGACACGGAGCGCCTCATTGT
  TGATTACTCTTGTGCACTCCAAAGAGACATTCTTCTTCAGGGCCGACTCTACCTCTCAGAAAATTGGATC
  TGCTTCTACAGCAACATCTTCCGCTGGGAAACCCTGCTGACAGTCCGTTTGAAAGATATCTGCTCCATGA
  CTAAAGAGAAAACAGCTCGCCTCATTCCCAATGCCATCCAAGTCTGCACTGATTCAGAAAAGCACTTTTT
  TACTTCCTTTGGAGCCCGGGACAGGACGTACATGATGATGTTCCGGCTCTGGCAGAATGCTCTCCTTGAA
  AAGCCCCTATGTCCCAAGGAGCTCTGGCACTTTGTCCACCAGTGCTACGGGAACGAGCTGGGCCTAACCA
  GTGATGATGAGGACTACGTGCCTCCAGATGATGACTTCAACACAATGGGCTACTGTGAGGAGATCCCCAT
  AGAAGAGAATGAAGTGAATGACAGCTCATCCAAAAGTAGCATCGAGACCAAGCCGGATGCCAGCCCACAG
  CTGCCCAAGAAGTCCATTACCAACAGCACACTGACGTCTACGGGAAGCAGTGAAGCCCCTGTTTCGTTTG
  ATGGCTTGCCATTGGAGGAGGAGGTGATGGAGGGGGATGGTTCCCTAGAGAAGGAGCTTGCCATTGACAA
  CATCATAGGGGAGAAGATCGAGATCATGGCGCCTGTGACCTCCCCTTCGCTGGACTTCAATGACAATGAG
  GATATCCCCACCGAGCTCAGTGATTCTTCAGATACCCACGATGAAGGAGAGGTCCAGGCCTTCTATGAGG
  ACCTGAGTGGAAGACAATATGTGAATGAGGTCTTCAACTTCAGCGTGGACAAACTCTATGACCTGCTGTT
  CACCAACTCACCCTTCCTGCGGGATTTCATGGAGCAGCGGCGTTTCTCTGATATCATCTTCCATCCATGG
  AAAAAGGAGGAGAATGGAAACCAGAGTCGAGTGATTCTCTACACTATCACCCTCACTAATCCTCTGGCTC
  CCAAAACAGCCACTGTCAGGGAGACACAGACCATGTACAAAGCGAGTCAGGAGAGCGAATGCTACGTGAT
  AGATGCTGAAGTCCTCACCCACGATGTGCCGTACCATGACTACTTCTACACCATCAATCGCTACACACTC
  ACCAGAGTGGCCCGGAACAAGAGCAGACTCAGGGTCTCCACAGAGCTCCGCTATCGAAAACAGCCCTGGG
  GGTTTGTGAAAACGTTCATCGAGAAGAACTTCTGGAGTGGACTGGAGGACTACTTCCGTCATTTAGAGAC
  TGAACTAACCAAAACAGAGAGTACCTACTTGGCTGAGATACACAGGCAGTCACCCAAGGAGAAGGCTAGC
  AAGTCTTCAGCAGTCCGGAGGAGGAAGCGTCCCCATGCCCACCTGCGGGTGCCTCACCTGGAAGAAGTGA
  TGAGTCCTGTCACCACACCCACTGATGAAGATGTGGGCCACAGGATCAAGCACGTGGCAGGTTCCACGCA
  GACACGGCACATCCCGGAGGACACTCCTGATGGTTTTCACCTACAAAGTGTGTCCAAGCTGCTGCTGGTT
  ATCAGCTGTGTTCTGGTGCTGCTGGTTGTCCTTAACATGATGCTCTTCTACAAGCTGTGGATGCTGGAAT
  ACACCACCCAGACCCTCACTGCCTGGCAGGGTCTCAGGCTCCAGGAAAGGTTACCCCAGTCTCAGACAGA
  ATGGGCCCAGCTATTAGAATCCCAACAGAAGTACCACGACACCGAGCTCCAGAAGTGGAGGGAAATCATC
  AAATCCTCAGTGTTGCTCCTGGACCAGATGAAGGACTCGCTTATCAATCTTCAGAATGGTATCAGGTCCC
  GAGACTACACAGCTGAGAGCGACGAGAAGAGGAACCGCTATCATTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172768
• Insert Size: 2637 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172768.1, NP_766356.1
• RefSeq Size: 2814 bp
• RefSeq ORF: 2637 bp
• Locus ID: 235283
• UniProt ID: Q80TI0
• Cytogenetics: 9 A5.1
• Gene Summary: Cholesterol transporter that mediates non-vesicular transport of cholesterol from the plasma membrane (PM) to the endoplasmic reticulum (ER) (PubMed:30220461). Contains unique domains for binding cholesterol and the PM, thereby serving as a molecular bridge for the transfer of cholesterol from the PM to the ER (PubMed:30220461). Plays a crucial role in cholesterol homeostasis in the adrenal gland and has the unique ability to localize to the PM based on the level of membrane cholesterol (PubMed:30220461). In lipid-poor conditions localizes to the ER membrane and in response to excess cholesterol in the PM is recruited to the endoplasmic reticulum-plasma membrane contact sites (EPCS) which is mediated by the GRAM domain (PubMed:30220461). At the EPCS, the sterol-binding VASt/ASTER domain binds to the cholesterol in the PM and facilitates its transfer from the PM to ER (PubMed:30220461).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.