Vps53 (NM_026664) Mouse Untagged Clone

CAT#: MC221945

Vps53 (untagged) - Mouse vacuolar protein sorting 53 (yeast) (Vps53), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Vps53
• Synonyms: 2010002A08Rik; 2310040I21Rik; 3100002B05Rik; Hccs1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221945 representing NM_026664
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGAGGAGGAGGAACTGGAGTTTGTGGAGGAGCTGGAGGCTGTGCTGCAGCTCACGCCAGAGGTGC
  AACTCGCCATCGAGCAGGTATTCCCAAGCCAGGATCCTCTTGATCAAGCTGACTTCAATGCCGTTGAGTA
  TATCAATACTCTGTTCCCAACAGAGCAATCTCTGGCAAACATTGACGATGTTGTGAACAAAATTAGACTG
  AAAATAAGGAGACTGGATGACAATATCCGGACTGTCGTAAGAGGTCAGACAAATGTGGGGCAGGATGGAA
  GGCAAGCACTTGAAGAGGCCCAGAAAGCCATTCAGCAACTCTTTGGCAAAATCAAGGATATCAAAGACAA
  AGCAGAAAAGTCAGAACAAATGGTGAAAGAGATCACCCGAGATATTAAGCAGTTAGATCACGCCAAGCGC
  CACCTGACCACCTCAATCACGACCCTGAACCACCTGCACATGCTGGCAGGCGGGGTGGACTCCCTGGAAG
  CCATGACCAGGCGCAGACAGTATGGAGAAGTTGCTAATCTCCTTCAGGGTGTAATGAATGTTCTGGAGCA
  CTTTCACAAATACATGGGAATCCCACAGATCCGACAGCTTTCTGAAAGGGTGAAGGCTGCACAGACAGAG
  TTAGGACAGCAAATCCTGGCTGACTTTGAAGAAGCATTTCCTTCGCAGGGTACCAAGAGGCCGGGAGGAC
  CCAGCAATGTCCTGAGAGATGCGTGCCTGGTGGCTAATATTTTGGACCCCAGAATCAAACAAGAAATCAT
  CAAAAAGTTTATTAAACAGCATCTATCAGAGTATCTAGTACTTTTTCAAGAAAACCAAGATGTCGCCTGG
  CTGGACAAAATTGACAGACGTTATGCTTGGGTGAAACGCCAGCTTGTGGACTATGAAGAGAAGTATGGCC
  GGATGTTTCCTCGGGAGTGGTGCATGACGGAGAGGATCTCAGTAGAGTTTTGCCACGTGACGAGGGCAGA
  ACTTTCCAAAATCATGCGTGCCAGAGCTAAGGAAATTGAAGTGAAATTGCTTCTTTTTGCGATCCAGAGA
  ACAACCAACTTTGAGGGGTTTCTTGCAAAACGCTTCTCCGGTTGCACCCTGACAGATGGAACCCTGAAAA
  AGCTGGAGTCCCCACCACCATCTACCAATCCTTTCCTGGAAGATGAGACAACACCAGAGATGGAGGAGCT
  GGCTCTGGAGAAAGGAGAGTTAGAGCAACCGAAGAAGCCCAAGGCCCCGGACAACCCGTTCCACGGCATC
  GTTTCCAAGTGTTTTGAGCCTCATCTCTACGTGTACATTGAGTCCCAGGACAAAAACCTCAGCGAGCTGA
  TAGACCGGTTTGTGGCAGATTTCAAAGCCCAGGGCCCACCCAAGCCCAACACTGACGAAGGGGGTGCTGT
  GCTCCCCAGCTGCGCTGACCTCTTTGTCTACTACAAGAAGTGCATGGTGCAGTGCTCACAGCTCAGCACA
  GGCGAGCCCATGATCGCCCTGACTACCATCTTCCAGAAGTACCTCCGAGAATACGCCTGGAAAATCCTCT
  CTGGCAACCTGCCTAAAACCAGCAGCAGCAGTGGGGGGCTGACCATCAGCAGCCTCCTCAAAGAGAAGGA
  GGGCTCAGAAGTGGCCAGGTTCACTCTGGAGGAGCTGTGTCTCATCTGCAGCATCCTGAGCACAGCCGAG
  TATTGTCTGGCTACCACCCAGCAGCTAGAAGAAAAACTCAAAGAAAAAGTTGATGTGAGCCTGACTGAAC
  GAATTAATCTCACAGGAGAAATGGACACATTCAGCACTGTCATCTCCAGCAGCATCCAGCTGTTGGTTCA
  AGATCTGGATGCAGCCTGTGACCCTGCCCTGATTGCCATGAGCAAGATGCCTTGGCAGAACGTGGAGCAT
  GTTGGTGACCAGAGCCCTTATGTCACTTCTGTTATTCTTCACATTAAGCAAAACGTCCCCATCATCCGTG
  ACAACCTGGCTTCCACACGGAAATACTTCACACAGTTCTGCATTAAATTTGCAAATTCGTTCATTCCCAA
  ATTCATCACCCACCTCTTCAAGTGTAAGCCGATCAGCATGGTGGGAGCAGAGCAGCTTCTGCTGGACACC
  CACTCCCTGAAGATGGTCCTGCTCGACCTTCCGTCCATTGGCTCCCAGGTGGTGAGGAAGGCCCCTGCCA
  GTTACACTAAGATTGTCGTGAAAGGCATGACCCGGGCTGAAATGATCCTTAAGGTGGTGATGGCCCCTCA
  TGAACCGTTGGTGGTATTTGTTGACAACTACATCAAACTCCTTACGGACTGCAACTCAGAAACCTTCCAG
  AAAATACTGGATATGAAGGGGCTGAAGAGGAGTGAGCAGAGCAGCATGCTGGAACTTTTGCGGCAGAGGC
  TCCCTGCCCCGCCCTCGGGGACAGAAGGTTCCAGCACCCTGTCGCTGATTGCCCCAACACCGGAGCAGGA
  GTCATCCCGAATCCGCAAACTGGAGAAACTGATTAAAAAGAGACTGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_026664
• Insert Size: 2499 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026664.3, NP_080940.2
• RefSeq Size: 2816 bp
• RefSeq ORF: 2499 bp
• Locus ID: 68299
• UniProt ID: Q8CCB4
• Cytogenetics: 11 B5
• Gene Summary: Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD. Acts as component of the EARP complex that is involved in endocytic recycling. The EARP complex associates with Rab4-positive endosomes and promotes recycling of internalized transferrin receptor (TFRC) to the plasma membrane.[UniProtKB/Swiss-Prot Function]