Arhgap44 (NM_175003) Mouse Untagged Clone

CAT#: MC221458

Arhgap44 (untagged) - Mouse Rho GTPase activating protein 44 (Arhgap44), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Arhgap44
• Synonyms: 6330543G20; AI840762; AU040829; AW493732; Rich2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221458 representing NM_175003
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAAGCAGTTTAACCGCATGCGCCAGTTGGCCAACCAGACGGTGGGCAGGGCAGAAAAGACTGAAG
  TTCTGAGCGAAGACCTTCTCCAGGTGGAGAAGCGCCTAGAGCTGGTGAAGCAGGTGTCCCACAGCACACA
  CAAGAAGCTCACCGCATGTCTGCAGGGTCAGCAGGGAGCAGAGGCCGACAAGCGATCAAAAAAGTTGCCT
  CTGACGACTCTGGCGCAGTGCTTGGTGGAGGGGTCCGCGATCCTGGGCGATGACACGCTTCTTGGGAAGA
  TGCTGAAGCTCTGCGGAGAGACGGAGGACAAGCTAGCTCAGGAGCTGATCCACTTTGAGCTGCAAGTGGA
  GAGGGATGTGATCGAGCCCCTGTTCCTGCTCGCTGAGGTGGAAATTCCAAATATTCAAAAGCAGAGGAAA
  CATTTAGCCAAGCTGGTGCTTGACATGGACTCTTCCCGGACCAGGTGGCAGCAGACTTCCAAGTCTTCAG
  GGCTTTCCAGCAGCCTTCAGCCTGCGGGTGCCAAAGCCGATGCCCTCAGGGAGGAGATGGAGGAGGCTGC
  CAACAGAGTGGAGATTTGCCGGGACCAGCTTTCTGCTGACATGTACAGTTTTGTGGCCAAAGAAATTGAC
  TATGCAAACTACTTTCAAACGCTAATAGAAGTGCAAGCTGAGTACCACAGGAAGTCGCTGACACTGCTGC
  AGGCTGTGTTGCCTCAGATCAAAGCCCAGCAGGAGGCCTGGGTGGAGAAGCCTTCCTTTGGGAAGCCCCT
  GGAGGAGCACCTGATGATCAGTGGCCGAGAGATCGCCTTCCCTATCGAGGCGTGTGTGACCATGCTGCTG
  GAGTGTGGGATGCAGGAGGAGGGCCTCTTCCGGGTAGCCCCCTCAGCCTCCAAGCTGAAGAAGCTGAAAG
  CTGCTCTGGACTGCTGTGTGGTGGATGTCCAGGAGTACTCGGCTGACCCCCACGCCATAGCAGGGGCTTT
  GAAGTCTTATCTCCGAGAGTTGCCAGAGCCTCTTATGACCTTTGAACTCTATGACGAATGGATCCAGGCT
  TCTAATATCCAGGAGCAAGACAAGAGACTTCAGGCTCTCTGGAATGCTTGTGAGAAGTTGCCCAAGGCCA
  ACCATAACAACATCAAATACTTGATCAAATTTTTATCGAAACTGTCTGAATATCAGGATGTAAACAAGAT
  GACTCCCAGTAACATGGCAATTGTCCTGGGACCCAATCTCCTTTGGCCACAGTCAGAGGGGAACATCACC
  GAGATGATGACCACAGTTTCCCTGCAGATCGTTGGCATCATCGAGCCCATTATCCAGCACGCAGACTGGT
  TCTTCCCTGGAGAGATAGAGTTCAACCTCACAGGCAGTTACGGGAGTCCAGTCCACGTGAACCACAATGC
  CAACTACAGCTCAATGCCCTCCCCAGACATGGACCCTGCTGACCGGCGCCAGCCAGAGCAGGCCCGCCGG
  CCCCTCAGTGTTGCCACAGACAACATGATGCTGGAGTTTTACAAGAAGGATGGCATGGGTGTGAGGGTCA
  TGGACACATCTTGGGTGGCACGAAGAGGCTCCTCTGCTGGTCGGAAAGCATCCTGCGCCCCGCCTTCCAT
  GCAGCCTCCTGCCCCACCCTCCGAGCTGGCTGCACCCTTGCCTTCACCCCTGCCGGAGCAGGTCCCAGAC
  AGCCCTGCCACTCCAGCTCCTGCGCTCTCTCCATCAGGTGCCAGCCTGCAGCCCACCCCCGAGCGCCCCA
  GTGTGTCTAAAAGCAAGGAACTGTCGCCAGGGTCTGGGCAGAAAGGAAGTCCAGGTTCCATCCAGGGGAC
  ACCCTGCCCAGGGACGCAACTGGGGCCACAGCCGGCTGCCAGCCCCAGCCAGCTTCCTGCAGACCAGAGT
  CCTCACACCCTCCGGAAAGTTTCAAAGAAGGTGGCACCAATTCCTCCCAAGGTCCCCTTCGTGCAGCCAG
  GGACTGTTTCAGATCAGCCCGTTGGCCAGCCATCCCCAGTCAGCCTGTCTCCTACTCCACCAAGCACCCC
  ATCCCCCTATGGACTGAGCTACCCTCCAGGCTACTCCATGGCCTCTGGCCAGCTTTCTCCTGCCTCAGCT
  CCTCCCCTGGCCTCTCCCTCTGTCTTTACAAGCACTTTAGCCAAATCTCGGCCCACCCCTAAGCCTCGAC
  AGAGGCCCACCCTGCCGCCTCCTCAGCCTCCCAGTGTCAGCCTGTCGGCTTCAAGTCCACAGTCCACAGA
  GCACCCCATGTTAGACGGCATGTCGCCGGGGGAAAGCATGTCTACAGCTGTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_175003
• Insert Size: 2295 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175003.3, NP_778168.2
• RefSeq Size: 4099 bp
• RefSeq ORF: 2295 bp
• Locus ID: 216831
• UniProt ID: Q5SSM3
• Cytogenetics: 11 B3
• Gene Summary: GTPase-activating protein (GAP) that stimulates the GTPase activity of Rho-type GTPases. Thereby, controls Rho-type GTPases cycling between their active GTP-bound and inactive GDP-bound states. Acts as a GAP at least for CDC42 and RAC1 (PubMed:24352656, PubMed:26969129). In neurons, is involved in dendritic spine formation and synaptic plasticity in a specific RAC1-GAP activity (PubMed:23739967, PubMed:24352656, PubMed:26969129). Limits the initiation of exploratory dendritic filopodia. Recruited to actin-patches that seed filopodia, binds specifically to plasma membrane sections that are deformed inward by acto-myosin mediated contractile forces. Acts through GAP activity on RAC1 to reduce actin polymerization necessary for filopodia formation (By similarity). In association with SHANK3, promotes GRIA1 exocytosis from recycling endosomes and spine morphological changes associated to long-term potentiation (PubMed:23739967).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) contains an alternate exon and differs in the 3' UTR, compared to variant 1. The resulting protein (isoform 2) is shorter and has a distinct C-terminus, compared to isoform 1.