Tmc1 (NM_028953) Mouse Untagged Clone

CAT#: MC221362

Tmc1 (untagged) - Mouse transmembrane channel-like gene family 1 (Tmc1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tmc1
• Synonyms: 4933416G09Rik; Beethoven; Bth; CWEA1; dn
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221362 representing NM_028953
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGCAAATCCAAGTGGAGGAGAAAGAAGAGGATACAGAGGAAAGCTCAAGTGAAGAAGAAGAAGATA
  AGCTACCCAGAAGAGAGAGCTTGAGACCAAAGAGGAAACGGACCAGAGATGTCATCAATGAGGATGACCC
  AGAACCGGAGCCGGAGGATGAAGAAACAAGAAAGGCAAGAGAAAAAGAAAGGCGGAGGAGGCTGCGGAGA
  GGAGCGGAAGAAGAAGAAGAAATTGATGAAGAGGAATTAGAACGGTTAAAAGCACTGCTCGATGAGAATA
  GACAAATGATCGCTACTGTCAAATGTAAACCTTGGAAAATGGAGAAGAAAATTGAAGTTCTCAAGGAAGC
  AAAGAAATTTGTGAGTGAGAATGAAGGCGCTCTTGGGAAAGGAAAGGGAAAGAAGTGGTTTGCATTTAAG
  ATGATGATGGCCAAGAAATGGGCAAAATTCCTCCGAGATTTTGAGAACTTCAAAGCGGCTTGCGTCCCAT
  GGGAAAACAAAATCAAGGCAATTGAAAGTCAGTTTGGTTCCTCAGTGGCCTCGTACTTCCTGTTCCTCAG
  GTGGATGTACGGCGTCAACATGGTTCTCTTTGTGTTGACCTTCAGCCTCATCATGTTACCGGAGTACCTC
  TGGGGTTTACCGTACGGCAGCTTACCTAGGAAAACAGTCCCAAGAGCTGAAGAAGCATCTGCAGCCAACT
  TTGGTGTGTTGTATGACTTCAATGGCCTGGCGCAGTACTCTGTCCTCTTTTATGGCTATTACGACAATAA
  ACGCACGATCGGATGGCTGAATTTCCGGCTACCTCTTTCCTACTTCCTGGTGGGGATTATGTGCATTGGA
  TACAGCTTCCTGGTTGTCCTCAAAGCGATGACCAAAAATATTGGTGACGATGGTGGTGGCGATGACAACA
  CTTTCAACTTCAGCTGGAAGGTGTTCTGTAGCTGGGACTATCTGATTGGTAACCCTGAAACAGCCGACAA
  CAAGTTTAACTCTATCACGATGAACTTTAAGGAAGCCATCATAGAAGAGAGAGCCGCACAGGTGGAGGAG
  AACATCCACCTCATCAGATTTCTGAGGTTTCTCGCTAACTTCTTCGTGTTCCTCACACTTGGTGCAAGTG
  GATACCTCATCTTTTGGGCTGTGAAGCGATCCCAGGAGTTCGCCCAGCAAGATCCTGACACCCTTGGGTG
  GTGGGAAAAAAATGAAATGAACATGGTAATGTCCCTCCTGGGGATGTTCTGTCCCACCCTGTTTGACTTA
  TTTGCTGAACTGGAAGATTACCATCCTCTCATTGCTCTGAAGTGGCTCCTGGGGCGCATTTTTGCTCTTC
  TTCTAGGCAACTTGTATGTATTCATTCTCGCCTTGATGGATGAGATTAACAACAAGATTGAAGAGGAGAA
  GCTTGTGAAGGCCAATATTACCCTGTGGGAAGCCAACATGATTAAGGCTTACAATGAATCTCTCTCTGGG
  CTCTCTGGGAACACCACAGGAGCACCCTTTTTCGTTCATCCTGCAGATGTCCCTCGCGGTCCCTGCTGGG
  AAACAATGGTGGGGCAGGAATTCGTGCGTCTCACCGTTTCTGACGTCCTGACCACTTACGTCACGATCCT
  CATTGGCGACTTCCTCAGAGCATGTTTCGTGAGGTTCTGCAATTACTGCTGGTGCTGGGACTTAGAATAT
  GGATATCCTTCATACACAGAATTCGACATCAGTGGCAACGTCCTCGCTCTGATCTTCAACCAAGGCATGA
  TCTGGATGGGCTCCTTCTTCGCTCCTAGCCTCCCGGGCATCAACATCCTCCGTCTCCACACATCCATGTA
  TTTCCAGTGCTGGGCTGTGATGTGCTGCAATGTTCCCGAGGCCAGGGTGTTCAAAGCTTCCAGATCCAAC
  AACTTCTACCTCGGCATGCTGCTACTCATCCTCTTCCTGTCCACCATGCCGGTCCTGTACATGATCGTCT
  CCCTCCCGCCATCTTTTGATTGTGGGCCCTTCAGTGGTAAAAACAGGATGTTTGAAGTCATCGGTGAGAC
  CCTGGAACATGACTTCCCAAGCTGGATGGCGAAGATCCTGAGGCAGCTTTCTAACCCCGGCCTTGTCATT
  GCTGTCATTCTGGTGATGGTCCTGACCATCTATTATCTCAATGCTACTGCCAAGGGCCAGAAAGCAGCGA
  ATCTGGACCTCAAAAAGAAGATGAAACAGCAAGCTTTGGAGAACAAAATGCGAAACAAGAAAATGGCAGC
  GGCTCGAGCAGCTGCAGCTGCTGGTGGCCAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_028953
• Insert Size: 2274 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028953.2, NP_083229.1
• RefSeq Size: 4073 bp
• RefSeq ORF: 2274 bp
• Locus ID: 13409
• UniProt ID: Q8R4P5
• Cytogenetics: 19 13.98 cM
• Gene Summary: Probable ion channel required for the normal function of cochlear hair cells.[UniProtKB/Swiss-Prot Function]