Phtf1 (NM_001163468) Mouse Untagged Clone

CAT#: MC220743

Phtf1 (untagged) - Mouse putative homeodomain transcription factor 1 (Phtf1), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Phtf1
• Synonyms: AU041898; AW049785; Phft; Phtf
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220743 representing NM_001163468
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCAAATGAGAGAGATGCTATATCATGGTATCAGAAGAAGATTGGAGCATATGATCAGCAGATAT
  GGGAAAAGTCAATCGAACAGACTCAGATTAAGGGTTTCAAAAACAAACCAAAAAAAATGGGCCATATAAA
  ACCAGACTTGATTGATGTTGACTTAATCAGAGTCACAGCGGTTGTGTTGTATCTCCTGATGCCTATTGTG
  AGCGCCAGTGAAGTGCTTGGACCCTTGTGCCTTATGCTACTCATGGGAACTGTCCACTGTCAGATTGTGT
  CGACTCAGATAACAAGGCCGTCAGGAAACAATGGAAATCGGAGAAGAAGAAAATTACGAAAAACTGTAAA
  TGGTGATGGGAGCCGAGACAATGGAAATAACTCCCCTGATAAAGTCAGAGCAGTAGAAACTCTGGAGTCT
  GCATCCAGTGTTGGTGGTTTTTGGGGGACTCTCTTTGGCAACAGGATTAAAAGGGTGAAATTAGTATCTA
  ACAAAGGTACTGAAACTGACAATGACTCAGGCTGTTTCCATCCTATCCTTAAGAAGAGACAGGGTCGACC
  CGAGATCAGAATGTGGCAAGCAAGAGAGAAAGCGAAGGTTTCGGATGGAGAAAAGTGCCGTAGGGAGGCT
  TATAGGCGTTTGGGTAATGGAATTTCAGACGATCTGTCAAGTGAGGAGGATGGTGAAGCACGGACACAGA
  TGATATTATTGCGTAGGAGTGTGGAAGGGGCGTCAAGTGACAATGGTTATGAAGTTAAGAATAGAAGGTC
  AATACTTTCAAGGCACCTAAATTCTCAGGTAAAGAAAACCACAAGGTGGTGTCATATTGTTCGGGATTCA
  GATAGTCTGGCTGAATCAGAGTTTGAATCAGCAGTCTTCAGCCAGGGTTCTAGATCTGGTATGAGTGGTG
  GCTCTCGGAGCCTTAACTTGTCAAGAAGAGACTCAGAAAGCACCCGACATGACTCCGAGACTGAGGATAT
  GTTATGGGATGACCTTCTCCATGGTCCAGAGTGCCGGTCATCTGTCACCAGTGACAGTGAGGGGGCTCAT
  GTAAATACCATTCATTCAGGGACCAAACGTGACCCCAAAGAAGATGTTTTTCAGCAGAACCATTTATTCT
  GGCTTCAGAATTCAAGTCCTGCCTCTGAGCGAGTTAGTGCGATAATCTGGGAAGGAAACGAGTGCAAAAA
  GATGGACATGTCTGTGTTGGAGATAAGCGGCATCATCATGAGCAGGGTCAATGCATACGAGCAAGGTGTT
  GGCTATCAGATGCTGGGGAATGCAGTCACCGTTGGCTTAGCACTTTTCCCATTTTTATATCGACTTTTCC
  GAGAGAAGAGCTTTGACCAACTAAAGTCCATCTCAGCAGAGGAGGTTCTGACCCTCTTCTGTGGTGCGCC
  ACCCGTCACACCTGTTGTTATTTTGTCTATAATTAATTTTATTGAAAGATTGTGTCTTACGTGGATGTTT
  TTTTTCATGATGTGTGTGGCAGAGAGAACATATAAGCAGAGATTTTTATTTGCAAAACTCTTCAGCCATA
  TTACTTCTGCCAGGAAGGCCAGGAAATATGAAATACCTCATTTCAGACTTAAAAAGGTGGAGAACATTAA
  AATATGGTTATCCCTGCGCTCCTATCTAAAGAGGCGGGGTCCACAGCGCTCAGTGGACGTGGTTGTGTCC
  TCCGTCTTCTTACTGACACTTTCAATTGCTTTCATTTGTTGTGCACAGGTTCTTCAAGGTCACAAGACAT
  TCCTGAATGATGCTTACAACTGGGAGTTCTTGATCTGGGAAACAGCATTGCTGCTGTTTTTGCTTCGCCT
  GGCCTCACTGGGGTCAGAAACCAACAAGAAGTACAGCAATGTTTCCATCCTGCTCACTGAACAGATTAAT
  TTATATCTTAAAATGGAAAAAAAGCCAAATAAGAAAGAACAGCTCACGCTAGTGAATAATGTATTGAAGC
  TGTCCACAAAGCTGTTAAAGGAGCTGGACACACCATTTAGACTCTATGGACTGACCATGAACCCCTTAAT
  CTACAACATCACAAGAGTAGTCATCCTTTCTGCTGTCTCTGGAGTTATAAGCGACCTTCTAGGATTCAAC
  ATAAGACTGTGGAAAATTAAGTCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001163468
• Insert Size: 2127 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163468.1, NP_001156940.1
• RefSeq Size: 3183 bp
• RefSeq ORF: 2127 bp
• Locus ID: 18685
• UniProt ID: Q9QZ09
• Cytogenetics: 3 F2.2
• Gene Summary: May play a role in transcription regulation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks an alternate in-frame coding exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.