Usp43 (BC021474) Mouse Untagged Clone

CAT#: MC220699

Usp43 (untagged) - Mouse ubiquitin specific peptidase 43 (cDNA clone MGC:38313 IMAGE:5343429), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Usp43
• Synonyms: 9130017K11Rik, B930051C24, RP23-464J2.1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC021474
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTGCAGAGGAAGGAGGTGTCCCTGCAGAGGAGGTGATCTTGGTTGAACTGTATCCCAATGGATTCC
  AGCGGTCATTCTTTGATGAAGAGGACCTGAATACCATTGCAGAGGGTGACAATGTGTATGCTTTCCAAGT
  CCCTCCCTCACCTGGTCTGGGGACGCTCTCAGCCCATCCGTCTGGTCTGTCAGTGTCACCGCGCTTGCCA
  GTCCGTGACAGCCAGCGATTCTCTGGGCCTCTCCACAGCGAGAACAGGGTGGTCTTTCTCTTCTGTAACC
  TGGTGGGCTCAGGGCAGCAGGCGAGCAGGTTTGGACCACCGTTCTTGATTCGGGAAGATAGAACAATCTC
  CTGGGCCCAGCTTCAACAGTGTATTCTCAGCAAGGTTCGATGCCTCATGAGGAGTGAGGTCTCTGCACAG
  GACCTGGGGACTCTGTTCTCCATCCGGGTTGTGGGGCTCTCCTTGGCCTGCAGCTACTTATCTCCAAAGG
  ACAACCGGCCCCTTTGCCACTGGGCAGTTGACAGGGCTTTGCACCTCAGGAGGCCAGGAGGCCCTCCTCA
  TGTCAAGCTGGCTGTGGAATGGGACAGCTCTGTCACAGAGCGCCTGTTTGGGAGCCTCCAGGAGGAACGG
  GTCCAGGATGCAGACAGTGTGTGGCGGCAGCAGCAGGCACACCAGCAGCCCAGCTGTACCCTGGACGAAT
  GTTTTCAGTCCTATACCAAGGAGGAACAGCTGGCCCAGGATGATGCATGGAAGTGCCCTCACTGCCAAGT
  CCTCCAGCAGGGTGTGGTCAAGCTGAGTCTGTGGACTCTGCCCGACATCCTGATCATCCATCTGAAACGG
  TTCTGTCAGGTGGGAGAGAGAAGGAACAAGCTCTCCACGCTGGTGAAGTTCCCACTGTCTGGACTCAACA
  TGGCTCCCCATGTAGCACGGAGGAGCACCAACTCCAAGGCGGGGCCCGGCCCTTGGTCCTCCTGGAAGCA
  GCCGATCTGCCTCCCCACCACCTACCCGCTGGACTTCCTCTACGACTTGTATGCTGTCTGCAACCACCAT
  GGCAGTCTGCAAGGTGGGCATTACACAGCCTATTGCCGGAACTCCCTGGACGGCCAGTGGTACAGCTATG
  ACGACAGCACAGTGGAGGCCCTGCGAGAGGATGAGGTCAACACCAGGGGGGCTTACATCCTATTCTACCA
  GAAGCGGAACAGCATCCCTCCCTGGTCAGCCAGCAGCTCCATGAGAGGTTCCACCAGCTCCTCCTTGTCT
  GATCATTGGCTCATGAGACTCGGGAGCCTCAACAACAGCACAAGGGGAAGCTTGCTGTCCTGGAGCTCGG
  CCCCCTGCCCCTCCGTGGCCCGGGTACCCGACTCTCCTGTCTTCACCAATGGTGTCTGCCACCAGGACAA
  GGGAGGAGTTGAGACCAGACCTTTGGTTCGGGGTGTTGGTGGCCGAAGTATCAGCATGAAGGCATCTCCT
  GCTTCCCGATCCAGGCATGGGCCCTTCAAGACCATGCCCCTGAGGTGGTCATTTGGACACAGGGAGAAAC
  GTCCCGGGGCATCCGTTGAACTGGTGGAATACCTAGAGTCCAGAAGAAGACCCCGTTCTACCAGCCAGTC
  CATTGTGCCGCTCTTGACTCGTGCTGCTGGTGGGGAAGAGACTTCAGCGTCACCGAGGTCAGATGGCACC
  CTTCCTGCTAAAAGTGAAGACAGCGGTCGAGCCATTGGGCAAGGAACAACTGGGGTGCCCCTTTCCTCAT
  GCCACCTCAACCACCATCCAGCCCTGGGGTCTTTAGATGACAGTCTGCACACTGCCAGGACACGAACGGG
  GAATGTAAGTCAGGATATCAGGCTCCCAAAAAAGTTTGACCTGCCCCTCACTGTGATGCCCTCAGTGGGG
  GATGAAAAGCCAGCTCGCCCTGAGGGCCAGAAGATGACACCTTGGAAGGGAAGTAGCCAGGTGGGAAGCC
  AGAGTAGCCCACCTTCCCCCTCCACTGGCTTGCTTAGAAACTTTAAGGACAATGGCCCAGGTACCTTACC
  CAAGATGAAGTCTAAGGCTGCCATGGAGGAAAGGGCCCCTGGCAAAGACAGGGGACAGGGGACATTCACT
  CTTTTGAAGTCTGTGTTTTGGAAGAAAGAACACAAGAGGACTGTCAGGACTGAGAGCTCACCACCAGCAC
  CCCCAATCTCCCTAGGGAGTGACCGGCTGAGCCCTGCCATGAATGAGCAGGCCCGAGGTTCATCCCCTGC
  CCTCAGGATTCGAGAGAGCCCAGCCAAGGGCCTGGGCAACCACATGGAGAGGGACATCAGATCTGCGCCC
  AGCTCTCTCCACCTCCCCCGAAAAGCCAGCAGGCCCCCTCGAGCCAGTACAGCTGGCACCTCACAAAGGA
  CCATTCCTGGGGAACAGATTTCTTATGGCACCCTGCAGAGAGTGAAATACCACACCCTTTCCTTAGGTCG
  AAAGAAATCCTTACCTGAGTCCAGCTTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC021474
• Insert Size: 2481 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC021474, AAH21474
• RefSeq Size: 2948 bp
• RefSeq ORF: 2480 bp
• Locus ID: 216835
• Cytogenetics: 11 B3
• Gene Summary: May recognize and hydrolyze the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins (By similarity).[UniProtKB/Swiss-Prot Function]