Poli (NM_001136090) Mouse Untagged Clone

CAT#: MC220331

Poli (untagged) - Mouse polymerase (DNA directed), iota (Poli), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Poli
• Synonyms: Rad30b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220331 representing NM_001136090
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCTCCAACCCAGAATTAAAGGACAGACCTTTAGGTGTGCAACAGAAATACTTGGTGGTTACCTGCA
  ACTATGAAGCCAGGAAACTGGGTGTGAGGAAGCTTATGAATGTGAGAGATGCGAAGGAAAAGTGTCCTCA
  GCTGGTCCTCGTGAACGGAGAAGACCTGAGCCGCTACAGAGAGATGTCCTATAAGGTCACAGAATTGTTG
  GAGGAATTTAGTCCAGCTGTTGAGAGACTTGGATTTGATGAAAATTTTGTGGATCTAACAGAAATGGTGG
  AAAAAAGACTCCAGCAGCTTCCAAGTGAGGAGGTTCCGTCAGTGACTGTGTTCGGCCATGTTTACAATAA
  CCAGTCTGTGAACCTACACAACATCATGCATCGGAGACTTGTGGTTGGATCGCAGATTGCAGCAGAGATG
  CGGGAAGCCATGTATAATCAGCTCGGGCTCACAGGCTGCGCCGGAGTGGCTCCTAATAAACTCTTGGCAA
  AGTTAGTGTCTGGGGTTTTTAAACCAAATCAGCAGACGGTCTTACTACCTGAAAGTTGTCAACATCTCAT
  TCACAGTTTGAACCACATAAAGGAAATTCCTGGTATCGGCTATAAAACTGCCAAGCGTCTCGAAGTTCTG
  GGAATCAATAGTGTTCATGATCTCCAAACCTTTCCAATCAAAACATTAGAAAAAGAATTAGGAATTGCAA
  TTGCTCAGCGTATCCAGCAGCTCAGTTTTGGAGAGGACAAGTCTCCCGTCACACCGTCAGGGCCACCACA
  GTCCTTTAGTGAAGAAGATACGTTTAAAAAGTGTTCCTCAGAAGTGGAAGCTAAAGCTAAGATTGAAGAA
  TTACTTTCCAGCCTTTTGACCAGAGTATGCCAGGATGGAAGGAAGCCCCATACAGTAAGATTAGTCATCC
  GTCGGTACTCTGACAAACACTGTAATCGAGAGAGTCGTCAGTGCCCTATCCCATCCCACGTCATTCAGAA
  GTTAGGGACAGGAAATCATGACTCCATGCCTCCCCTGATTGATATCCTTATGAAACTTTTCCGAAATATG
  GTGAACGTGAAGATGCCCTTTCACCTGACTCTTATGAGCGTGTGCTTCTGCAACCTGAAAGCCCTGAGCA
  GTGCTAAGAAAGGGCCTATGGACTGCTACCTAACGTCCCTCTCAACCCCTGCCTACACCGACAAGCGCGC
  CTTTAAAGTGAAGGACACCCACACGGAAGACTCTCACAAGGAGAAAGAAGCAAACTGGGATTGTCTACCA
  AGTAGAAGAATCGAAAGCACAGGAACGGGGGAGTCTCCGTTGGATGCCACCTGTTTTCCTAAAGAAAAAG
  ACACAAGTGACTTGCCCCTCCAGGCACTGCCAGAGGGTGTTGATCAAGAAGTCTTTAAGCAACTTCCAGC
  AGATATTCAAGAAGAAATCCTTTCTGGAAAATCTAGAGAAAATCTAAAAGGGAAAGGAAGTTTAAGTTGT
  CCGCTGCATGCCTCTAGAGGAGTATTGTCTTTCTTTTCTACAAAGCAAATGCAAGCCAGTCGCTTAAGCC
  CCAGAGATACTGCGCTCCCTAGCAAGCGGGTATCAGCTGCATCTCCCTGTGAGCCGGGAACGTCAGGACT
  GAGCCCCGGGAGCACCTCCCATCCATCGTGCGGAAAGGACTGTTCCTATTACATAGACAGCCAGTTAAAG
  GATGAGCAAACGAGTCAAGGCCCTACTGAGTCTCAAGGATGCCAGTTTTCCAGCACGAACCCTGCTGTTT
  CTGGTTTCCATTCCTTTCCTAATCTGCAGACTGAACAGCTCTTCTCCACACATCGCACTGTAGACAGCCA
  CAAGCAGACAGCCACCGCCTCTCACCAGGGACTAGAAAGTCACCAGGGACTAGAAAGCAGAGAGCTGGAT
  TCTGCGGAAGAAAAGCTTCCTTTCCCACCAGACATTGATCCGCAAGTTTTCTATGAGCTTCCAGAAGAGG
  TCCAAAAGGAACTGATGGCGGAATGGGAGAGAGCTGGAGCTGCGCGCCCCTCGGCGCACAGATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001136090
• Insert Size: 2025 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001136090.2, NP_001129562.1
• RefSeq Size: 2937 bp
• RefSeq ORF: 2025 bp
• Locus ID: 26447
• UniProt ID: Q6R3M4
• Cytogenetics: 18 E2
• Gene Summary: Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 2. This results in an isoform (1) that has a shorter N-terminus, compared to isoform 2.